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[180337_at]

Description Y54E5A.3 /REP_DB=WormBase Gene ID /WP=CE24455 /TR=Q9U246 /GB=CAA21661.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 96      4grp_total > 05      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 117
1 2 3 4 range
A 77.9
(0.2)
A 		30.1
(-3)
A 		49.4
(-1.7)
A 		120.1
(3)
A 		90
B 24.5
(-2.1)
A 		11.1
(-2.9)
A 		9.6
(-3)
A 		104.9
(3)
A 		95
C 52.7
(-0.9)
A 		17.8
(-2.7)
A 		11.4
(-3)
A 		126.9
(3)
A 		116
avr 51.7
(-1) 		19.7
(-3) 		23.5
(-2.8) 		117.3
(3) 		98

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 98
1 2 3 4 5 6 range
A 77.9
(-2.3)
A 		73.5
(-2.8)
A 		71.3
(-3)
A 		131.1
(3)
A 		80.4
(-2.1)
A 		115.6
(1.4)
A 		60
B 53.4
(-2.1)
A 		66.4
(-1.3)
A 		72.6
(-0.9)
A 		39.1
(-3)
A 		129
(2.5)
A 		137.1
(3)
A 		98
C 45.9
(-3)
A 		77.4
(-0.7)
A 		65.4
(-1.6)
A 		121.1
(2.4)
A 		122.9
(2.5)
A 		129.4
(3)
A 		84
avr 59.1
(-3) 		72.4
(-1.8) 		69.8
(-2.1) 		97.1
(0.3) 		110.8
(1.5) 		127.4
(3) 		68
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 26
1 2 3 4 range
A 28.4
(3)
A 		6.5
(-3)
A 		12.9
(-1.2)
A 		17.6
(0)
A 		22
B 7.8
(-1.3)
A 		2.7
(-2.9)
A 		2.2
(-3)
A 		22.4
(3)
A 		20
C 17.5
(1.7)
A 		5.1
(-2.6)
A 		4
(-3)
A 		21.4
(3)
A 		17
avr 17.9
(2) 		4.8
(-3) 		6.4
(-2.4) 		20.5
(3) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 42
1 2 3 4 5 6 range
A 14.4
(-3)
A 		26.2
(0.7)
A 		15.5
(-2.7)
A 		33.3
(3)
A 		24.3
(0.1)
A 		28.1
(1.3)
A 		19
B 18.6
(-1.8)
A 		22.1
(-1.3)
A 		17.6
(-2)
A 		10.4
(-3)
A 		52.4
(3)
A 		51.1
(2.8)
A 		42
C 17
(-2.9)
A 		23.6
(-1.4)
A 		16.6
(-3)
A 		24.4
(-1.2)
A 		39.8
(2.2)
A 		43.3
(3)
A 		27
avr 16.7
(-3) 		24
(-1.2) 		16.6
(-3) 		22.7
(-1.5) 		38.8
(2.5) 		40.8
(3) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180337_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1029
Sequence Type Exemplar sequence
Representative Public ID Y54E5A.3 NCBI
Target Description Y54E5A.3 /REP_DB=WormBase Gene ID /WP=CE24455 /TR=Q9U246 /GB=CAA21661.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061150(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061150 NCBI Caenorhabditis elegans Y54E5A.3 (Y54E5A.3) mRNA, complete cds. 11/11 None
GENEFINDER00000041551 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14702288:14708418:1 11/11 None
SNAP00000041547 ENSEMBL cdna:SNAP chromosome:CEL140:I:14705390:14722870:1 11/11 None
Y54E5A.3 ENSEMBL cdna:known chromosome:CEL140:I:14704643:14708418:1 gene:Y54E5A.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14704573-14708349(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y54E5A.3
Entrez Gene 3565579 Entrez gene
SwissProt Q9U246 EMBL-EBI
WormBase CE24455 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA21661 Hypothetical protein Y54E5A.3 [Caenorhabditis elegans] ref|NP_493551.1| Y54E5A.3 [Caenorhabditis elegans] 0.0
blast CAA21656 Hypothetical protein Y54E5A.7 [Caenorhabditis elegans] ref|NP_493555.1| Y54E5A.7 [Caenorhabditis elegans] 0.0
blast CAA21661 Hypothetical protein Y54E5A.3 [Caenorhabditis elegans] ref|NP_493551.1| Y54E5A.3 [Caenorhabditis elegans] 1.0E-180
blast CAA21661 Hypothetical protein Y54E5A.3 [Caenorhabditis elegans] ref|NP_493551.1| Y54E5A.3 [Caenorhabditis elegans] 1.0E-146
blast CAE72564 Hypothetical protein CBG19748 [Caenorhabditis briggsae] 1.0E-137
blast CAE64140 Hypothetical protein CBG08756 [Caenorhabditis briggsae] 1.0E-113
blast CAA21660 Hypothetical protein Y54E5A.2 [Caenorhabditis elegans] ref|NP_493550.1| Y54E5A.2 [Caenorhabditis elegans] 1.0E-111
blast CAE64140 Hypothetical protein CBG08756 [Caenorhabditis briggsae] 1.0E-102
Protein Domains
Database ID Description E-Value
Pfam IPR003877 EMBL-EBI SPla/RYanodine receptor SPRY 4.2E-38
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493551.1 1 364-386
Y54E5A.3 1 364-386
GENEFINDER00000041551 1 628-650
SNAP00000041547 1 278-300
Sequence
Target Sequence 
>C. ELEGANS:180337_AT
gtagctaacaaggctgctgggcacaaattcacgtctggaatgttcatcgaattagagtcc
tgcaagtcgaatattcaaaagacgaattgctacgagtgggccaagtgccacaatatttcg
atggaagacgtccacgtggaatccgaggagcaagtttccgattcgtgtcagcaagtgcaa
tttatcgatgtgctcgttggaatggataatatgaaagtgaccgtcggagcccaccaacat
ctccgtatccgaatggattcagaagttcacgatatgcgaaccacgtccgccaaatggaga
attgatcttcgaaaacccaaaaaatacgataagaaccgcccgtgtctgctgcagggaacc
gtgcaacgtggcggcgagctactcattcttgacttttccacgtggcacatgaaaattgat
ttgattttggcgaaggaacggaagaaggttcgaatcacttttcataatttaaaagttgac
gagaaggtttctgcaatacaaaatactgtattctattgggcgctggcaattgctgcattc
ttcctgcttctcgtcactgccgtcattttaatgcac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTAGCTAACAAGGCTGCTGGGCACA 192 455 598 Antisense
TGCTGGGCACAAATTCACGTCTGGA 408 583 612 Antisense
ATTGCTACGAGTGGGCCAAGTGCCA 412 5 683 Antisense
GACGTCCACGTGGAATCCGAGGAGC 14 379 724 Antisense
GTTTCCGATTCGTGTCAGCAAGTGC 527 449 751 Antisense
ACCAACATCTCCGTATCCGAATGGA 183 85 830 Antisense
TTCACGATATGCGAACCACGTCCGC 125 685 863 Antisense
ATACGATAAGAACCGCCCGTGTCTG 281 23 921 Antisense
CCCGTGTCTGCTGCAGGGAACCGTG 257 261 936 Antisense
TACTGTATTCTATTGGGCGCTGGCA 167 647 1101 Antisense
CGTCACTGCCGTCATTTTAATGCAC 156 247 1149 Antisense
Sequence Source Affymetrix Proprietary Database