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[180334_at]

Description F23C8.4 /REP_DB=WormBase Gene ID /WP=CE20713 /TR=Q9TXH9 /GB=AAD03130.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 89      6grp_high1_10_10 > 98      4grp_highest_8_8 > 12      4grp_high_8_8 > 39      6grp_highest_nonorm > 95      6grp_total > 96      4grp_total > 40      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 359
1 2 3 4 range
A 574.1
(-3)
P 		784.3
(1)
P 		887.3
(3)
P 		841.2
(2.1)
P 		313
B 528.8
(-3)
P 		841.5
(2.5)
P 		867.5
(3)
P 		833.1
(2.4)
P 		339
C 558.9
(-3)
P 		841.6
(3)
P 		841.3
(3)
P 		686.7
(-0.3)
P 		283
avr 553.9
(-3) 		822.5
(2.2) 		865.4
(3) 		787
(1.5) 		312

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 769
1 2 3 4 5 6 range
A 479.4
(-3)
P 		525.3
(-2.6)
P 		584
(-2.1)
P 		690.4
(-1.1)
P 		928.3
(1.1)
P 		1143.2
(3)
P 		664
B 543.4
(-1.9)
P 		410.7
(-3)
P 		621.6
(-1.3)
P 		716.7
(-0.6)
P 		964
(1.4)
P 		1162.7
(3)
P 		752
C 582.5
(-1.6)
P 		397.7
(-3)
P 		695.4
(-0.7)
P 		762.2
(-0.2)
P 		1166.9
(3)
P 		957.6
(1.4)
P 		769
avr 535.1
(-2.2) 		444.6
(-3) 		633.7
(-1.2) 		723.1
(-0.4) 		1019.7
(2.4) 		1087.8
(3) 		643
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 177
1 2 3 4 range
A 209
(1.7)
P 		170.2
(-0.4)
P 		232
(3)
P 		123.1
(-3)
P 		109
B 168.6
(-3)
P 		207.8
(3)
P 		201
(2)
P 		177.5
(-1.6)
P 		39
C 185
(-0.7)
P 		242
(1.3)
P 		293.2
(3)
P 		115.8
(-3)
P 		177
avr 187.5
(-0.2) 		206.7
(0.9) 		242.1
(3) 		138.8
(-3) 		103

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 345
1 2 3 4 5 6 range
A 88.4
(-3)
P 		186.8
(0.1)
P 		127.2
(-1.8)
P 		175.1
(-0.3)
P 		280.1
(3)
P 		278.3
(2.9)
P 		192
B 189.1
(-1.9)
P 		136.7
(-3)
P 		150.9
(-2.7)
P 		189.8
(-1.9)
P 		391.6
(2.2)
P 		433.6
(3)
P 		297
C 215.9
(-0.8)
P 		121.4
(-3)
P 		177
(-1.7)
P 		153.3
(-2.3)
P 		377.6
(3)
P 		320.6
(1.7)
P 		256
avr 164.5
(-2.5) 		148.3
(-3) 		151.7
(-2.9) 		172.7
(-2.3) 		349.8
(3) 		344.2
(2.8) 		202
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180334_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.315
Sequence Type Exemplar sequence
Representative Public ID F23C8.4 NCBI
Target Description F23C8.4 /REP_DB=WormBase Gene ID /WP=CE20713 /TR=Q9TXH9 /GB=AAD03130.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058577(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058577 NCBI Caenorhabditis elegans F23C8.4 (F23C8.4) mRNA, complete cds. 11/11 None
SNAP00000018138 ENSEMBL cdna:SNAP chromosome:CEL140:I:2434012:2435576:1 11/11 None
GENEFINDER00000018150 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2434012:2435576:1 11/11 None
F23C8.4 ENSEMBL cdna:known chromosome:CEL140:I:2434005:2435637:1 gene:F23C8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2434011-2435576(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F23C8.4
Entrez Gene 171804 Entrez gene
SwissProt Q9TXH9 EMBL-EBI
WormBase CE20713 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1603033_AT similar to DNA segment, Chr 19, ERATO Doi 721, expressed cfa
CANINE:1596338_AT similar to DNA segment, Chr 19, ERATO Doi 721, expressed cfa
CANINE:1591590_AT similar to DNA segment, Chr 19, ERATO Doi 721, expressed cfa
CANINE:1583522_S_AT similar to DNA segment, Chr 19, ERATO Doi 721, expressed cfa
CANINE_2:CFA.12119.1.A1_AT similar to DNA segment, Chr 19, ERATO Doi 721, expressed cfa
CANINE_2:CFA.13688.1.A1_S_AT similar to DNA segment, Chr 19, ERATO Doi 721, expressed cfa
CANINE_2:CFA.13688.1.A1_X_AT similar to DNA segment, Chr 19, ERATO Doi 721, expressed cfa
CANINE_2:CFAAFFX.24069.1.S1_S_AT similar to DNA segment, Chr 19, ERATO Doi 721, expressed cfa
DROSOPHILA_2:1625389_AT dm
DROSGENOME1:148369_AT dm
HG-U133_PLUS_2:210623_AT unknown protein LOC51035 hs
HG-U133A:210623_AT unknown protein LOC51035 hs
HG-U133A_2:210623_AT unknown protein LOC51035 hs
HG-U95AV2:37376_AT unknown protein LOC51035 hs
HUGENEFL:M68864_AT unknown protein LOC51035 hs
HG-U133A:201871_S_AT unknown protein LOC51035 hs
HG-U133_PLUS_2:201871_S_AT unknown protein LOC51035 hs
HG-FOCUS:201871_S_AT unknown protein LOC51035 hs
HG-U133A_2:201871_S_AT unknown protein LOC51035 hs
U133_X3P:G7705653_3P_A_AT unknown protein LOC51035 hs
MG-U74AV2:97239_AT DNA segment, Chr 19, ERATO Doi 721, expressed mm
MG-U74AV2:97241_AT DNA segment, Chr 19, ERATO Doi 721, expressed mm
MG-U74AV2:97240_G_AT DNA segment, Chr 19, ERATO Doi 721, expressed mm
MOE430A:1436342_A_AT DNA segment, Chr 19, ERATO Doi 721, expressed mm
MOUSE430A_2:1436342_A_AT DNA segment, Chr 19, ERATO Doi 721, expressed mm
MOUSE430_2:1436342_A_AT DNA segment, Chr 19, ERATO Doi 721, expressed mm
MU19KSUBB:TC26359_AT DNA segment, Chr 19, ERATO Doi 721, expressed (D19Ertd721e), mRNA mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
4190 aspartic-type endopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAD03130 Hypothetical protein F23C8.4 [Caenorhabditis elegans] ref|NP_490978.1| F23C8.4 [Caenorhabditis elegans] 1.0E-115
blast CAE60385 Hypothetical protein CBG03986 [Caenorhabditis briggsae] 1.0E-101
blast CAE57348 Hypothetical protein CBG00290 [Caenorhabditis briggsae] 1.0E-42
Protein Domains
Database ID Description E-Value
Pfam IPR000449 EMBL-EBI Ubiquitin-associated domain 4.1E-6
Pfam IPR001012 EMBL-EBI UBX domain 5.6E-19
Sequence
Target Sequence 
>C. ELEGANS:180334_AT
gctcggaagctatcaagcctctcactgcagaggaaaaggccgcaaaagtcctggaaatcc
gagaaaaaatcaaagtccatcaggccaaaaaggcaaagctcgaggccgaagagaatcgtg
agaaggagaaaaagcgtcgtgaggatggaaaagccatgatcagccataaagaagccgcca
gagatcgtgaaattcgagaagccgctcaagatcgtcgccgcgaaaagaacgaagacgaaa
tcgccagaaaacgagttctcgagcaaatccggcttgacaaggaagcccgtaaggcaaaag
cttcaggccaaccggttccagaggcaaagcctgctccgtctgcagctccagttgctccgc
caaaagactactcaactacaactcttcaattccgacttctcgatggacaaactgttagac
aacagtttgaagctaatgagccgctcgcaatggtccgcgcctgggtcgaaactaatcacg
caaacggtgttccgttcaccctgatgaccccattcccccgcaaagtgttcaccgaggatg
atatgggaacaccgctcaaagttctcaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTCGGAAGCTATCAAGCCTCTCAC 228 299 302 Antisense
GAGTTCTCGAGCAAATCCGGCTTGA 100 399 554 Antisense
GGCAAAAGCTTCAGGCCAACCGGTT 653 533 594 Antisense
CCAACCGGTTCCAGAGGCAAAGCCT 400 1 609 Antisense
TTGCTCCGCCAAAAGACTACTCAAC 67 703 653 Antisense
ACTACTCAACTACAACTCTTCAATT 560 101 668 Antisense
CTCTTCAATTCCGACTTCTCGATGG 498 229 683 Antisense
CTAATGAGCCGCTCGCAATGGTCCG 119 227 734 Antisense
CGCGCCTGGGTCGAAACTAATCACG 102 257 757 Antisense
CCCCCGCAAAGTGTTCACCGAGGAT 623 259 816 Antisense
GGGAACACCGCTCAAAGTTCTCAAT 550 493 846 Antisense
Sequence Source Affymetrix Proprietary Database