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[180329_at]

Description R12E2.5 /REP_DB=WormBase Gene ID /WP=CE18137 /TR=O61787 /GB=AAC17026.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 25      4grp_total > 42      6grp_cgc > 24      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 104
1 2 3 4 range
A 79.7
(2.8)
P 		76.5
(2.5)
A 		81.8
(3)
P 		23.3
(-3)
A 		59
B 69.8
(-2.8)
P 		93
(3)
A 		83.4
(0.6)
A 		68.9
(-3)
A 		24
C 58.4
(-3)
A 		120
(2.3)
A 		76.1
(-1.5)
A 		127.7
(3)
P 		69
avr 69.3
(-3) 		96.5
(3) 		80.4
(-0.6) 		73.3
(-2.1) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 137
1 2 3 4 5 6 range
A 138
(3)
P 		112.8
(1.5)
P 		137.2
(3)
P 		113
(1.5)
A 		45
(-2.5)
A 		37.3
(-3)
A 		101
B 130.7
(1.6)
P 		162.7
(3)
P 		73.4
(-0.9)
A 		75.1
(-0.8)
A 		25.3
(-3)
A 		91.9
(-0.1)
P 		137
C 103.8
(1.8)
P 		117.3
(3)
P 		59.4
(-1.9)
A 		52.2
(-2.6)
A 		47
(-3)
A 		74.1
(-0.7)
P 		70
avr 124.2
(2.6) 		130.9
(3) 		90
(0.3) 		80.1
(-0.3) 		39.1
(-3) 		67.8
(-1.1) 		92
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 31
1 2 3 4 range
A 29
(3)
P 		16.6
(0.1)
A 		21.4
(1.2)
P 		3.4
(-3)
A 		26
B 22.3
(2.5)
P 		23
(3)
A 		19.3
(0.3)
A 		14.7
(-3)
A 		8
C 19.3
(-3)
A 		34.5
(3)
A 		26.5
(-0.2)
A 		21.5
(-2.1)
P 		15
avr 23.5
(2.4) 		24.7
(3) 		22.4
(1.8) 		13.2
(-3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 45
1 2 3 4 5 6 range
A 25.4
(0.2)
P 		40.1
(3)
P 		29.9
(1)
P 		28.7
(0.8)
A 		13.6
(-2.1)
A 		9.1
(-3)
A 		31
B 45.5
(1.8)
P 		54.1
(3)
P 		17.8
(-2)
A 		19.9
(-1.7)
A 		10.3
(-3)
A 		34.3
(0.3)
P 		44
C 38.5
(3)
P 		35.8
(2.4)
P 		15.1
(-2)
A 		10.5
(-3)
A 		15.2
(-2)
A 		24.8
(0.1)
P 		28
avr 36.5
(1.7) 		43.3
(3) 		20.9
(-1.4) 		19.7
(-1.7) 		13
(-3) 		22.7
(-1.1) 		30
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180329_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1771
Sequence Type Exemplar sequence
Representative Public ID R12E2.5 NCBI
Target Description R12E2.5 /REP_DB=WormBase Gene ID /WP=CE18137 /TR=O61787 /GB=AAC17026.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058913(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058913 NCBI Caenorhabditis elegans INneXin family member (inx-16) (inx-16) mRNA, complete cds. 10/11 None
SNAP00000012679 ENSEMBL cdna:SNAP chromosome:CEL140:I:4162058:4164419:1 10/11 None
GENEFINDER00000012692 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4162058:4164750:1 11/11 None
R12E2.5 ENSEMBL cdna:known chromosome:CEL140:I:4162058:4164937:1 gene:R12E2.5 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4162057-4164750(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol inx-16
Ensembl R12E2.5
Entrez Gene 172005 Entrez gene
SwissProt O61787 EMBL-EBI
WormBase CE28765 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5921 gap junction inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_491314 INneXin family member (inx-16) [Caenorhabditis elegans] gb|AAC17026.2| Innexin protein 16 [Caenorhabditis elegans] 0.0
blast CAE66707 Hypothetical protein CBG12052 [Caenorhabditis briggsae] 0.0
blast AAB95049 Innexin protein 13 [Caenorhabditis elegans] ref|NP_491212.1| INneXin family member (inx-13) [Caenorhabditis elegans] 1.0E-66
Protein Domains
Database ID Description E-Value
Pfam IPR000990 EMBL-EBI Innexin 1.3E-37
Pfam IPR000990 EMBL-EBI Innexin 7.5E-55
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491314.1 4 28-45,105-127,176-198,264-286
R12E2.5 4 28-45,105-127,176-198,264-286
SNAP00000012679 4 28-45,105-127,176-198,264-286
GENEFINDER00000012692 4 28-45,105-127,176-198,264-286
Sequence
Target Sequence 
>C. ELEGANS:180329_AT
ttcctgctcaactcatttttaaacactgcttacacattttggggatggggaatcttttgg
gatatggtaaatggaaggcattggcaagagagtgggcactttccaagagtatcattctgc
gatattaatgtgagagaacttggaaacattcatcattggagtcttcaatgtgttctgatg
gttaatatgttcaatgaaaagatcttcattttcttgtggttctggttcgcatttttgctt
gtcgccactgcaggagattttgttatttgggtttggagacgatttgacagcaattcgaaa
cttggatttatcttggatcttttgaaccaagaaggaattgatcattctccacagaaagcc
agtgagctgtacaaaaacgtgcttcgtgatgacggagttctgttcctccggctcctcgat
tcgaatagcggacgactgaactcggaagagcttatgaagaaaatttataatatttcagtt
ggccatgcaactgatttgaatacacctattgaagaacatgcaactaaagcaactgacctg
aatcacagtgagatcatgggtttggaagagtttatggaaaattatccaactcacgctcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCCTGCTCAACTCATTTTTAAACA 58 701 598 Antisense
AACACTGCTTACACATTTTGGGGAT 575 135 619 Antisense
GTGGGCACTTTCCAAGAGTATCATT 250 489 689 Antisense
GGAGTCTTCAATGTGTTCTGATGGT 524 517 755 Antisense
GATCTTCATTTTCTTGTGGTTCTGG 534 419 798 Antisense
TCTGGTTCGCATTTTTGCTTGTCGC 705 607 818 Antisense
TGCTTGTCGCCACTGCAGGAGATTT 546 577 833 Antisense
GATCATTCTCCACAGAAAGCCAGTG 57 421 937 Antisense
GATGACGGAGTTCTGTTCCTCCGGC 474 395 985 Antisense
TCCGGCTCCTCGATTCGAATAGCGG 245 595 1004 Antisense
GGAAAATTATCCAACTCACGCTCCA 611 527 1173 Antisense
Sequence Source Affymetrix Proprietary Database