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[180325_at]

Description F14D2.2 /REP_DB=WormBase Gene ID /WP=CE16977 /TR=O44809 /GB=AAB94958.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 89      4grp_total > 07      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 52
1 2 3 4 range
A 51
(3)
A 		19.2
(-2.6)
A 		16.7
(-3)
A 		51.2
(3)
A 		35
B 68.5
(3)
A 		26.3
(-2.6)
A 		31.5
(-1.9)
A 		23
(-3)
A 		46
C 24.4
(-3)
A 		24.2
(-3)
A 		64
(3)
A 		40.1
(-0.6)
A 		40
avr 48
(3) 		23.2
(-3) 		37.4
(0.4) 		38.1
(0.6) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 80
1 2 3 4 5 6 range
A 30.2
(-3)
A 		37.6
(-2.1)
A 		33
(-2.7)
A 		30.5
(-3)
A 		39.4
(-1.9)
A 		78.9
(3)
A 		49
B 35.5
(-0.9)
A 		7.7
(-3)
A 		58.6
(0.8)
A 		69
(1.6)
A 		87.6
(3)
A 		38.6
(-0.7)
A 		80
C 54.8
(1.8)
A 		66.1
(3)
A 		12.8
(-2.7)
A 		9.9
(-3)
A 		16.4
(-2.3)
A 		30.9
(-0.8)
A 		56
avr 40.2
(-0.8) 		37.1
(-2.1) 		34.8
(-3) 		36.5
(-2.3) 		47.8
(2.3) 		49.5
(3) 		15
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 18.6
(3)
A 		4.2
(-3)
A 		4.4
(-2.9)
A 		7.5
(-1.6)
A 		14
B 21.8
(3)
A 		6.5
(-2.4)
A 		7.3
(-2.1)
A 		4.9
(-3)
A 		17
C 8.1
(-2.5)
A 		7
(-2.9)
A 		22.3
(3)
A 		6.8
(-3)
A 		16
avr 16.2
(3) 		5.9
(-3) 		11.3
(0.1) 		6.4
(-2.7) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 5.6
(-3)
A 		13.4
(0.4)
A 		7.2
(-2.3)
A 		7.7
(-2.1)
A 		11.9
(-0.2)
A 		19.2
(3)
A 		14
B 12.3
(-1.2)
A 		2.6
(-3)
A 		14.2
(-0.9)
A 		18.3
(-0.1)
A 		35.6
(3)
A 		14.4
(-0.9)
A 		33
C 20.3
(3)
A 		20.2
(3)
A 		3.3
(-2.6)
A 		2
(-3)
A 		5.3
(-1.9)
A 		10.3
(-0.3)
A 		18
avr 12.7
(-0.1) 		12.1
(-0.5) 		8.2
(-3) 		9.3
(-2.3) 		17.6
(3) 		14.6
(1.1) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180325_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4103
Sequence Type Exemplar sequence
Representative Public ID F14D2.2 NCBI
Target Description F14D2.2 /REP_DB=WormBase Gene ID /WP=CE16977 /TR=O44809 /GB=AAB94958.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062116(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062116 NCBI Caenorhabditis elegans F14D2.2 (F14D2.2) mRNA, complete cds. 11/11 None
SNAP00000037072 ENSEMBL cdna:SNAP chromosome:CEL140:II:3359141:3361349:1 11/11 None
GENEFINDER00000037092 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3359141:3359794:1 11/11 None
F14D2.2 ENSEMBL cdna:known chromosome:CEL140:II:3359141:3361172:1 gene:F14D2.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3359139-3359793(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F14D2.2
Entrez Gene 184453 Entrez gene
SwissProt O44809 EMBL-EBI
WormBase CE16977 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_494518 F14D2.1 [Caenorhabditis elegans] gb|AAB94956.1| Hypothetical protein F14D2.1 [Caenorhabditis elegans] 1.0E-149
blast NP_001022084 F14D2.15 [Caenorhabditis elegans] gb|AAT81201.1| Hypothetical protein F14D2.15 [Caenorhabditis elegans] 1.0E-145
blast T32791 hypothetical protein F14D2.8 - Caenorhabditis elegans 1.0E-145
blast NP_494517 F14D2.2 [Caenorhabditis elegans] gb|AAB94958.1| Hypothetical protein F14D2.2 [Caenorhabditis elegans] 6.0E-68
blast AAB70932 Hypothetical protein C41H7.4 [Caenorhabditis elegans] ref|NP_494422.1| C41H7.4 [Caenorhabditis elegans] 1.0E-49
blast AAB70937 Hypothetical protein C41H7.6 [Caenorhabditis elegans] ref|NP_494420.1| C41H7.6 [Caenorhabditis elegans] 1.0E-33
Protein Domains
Database ID Description E-Value
Pfam IPR005020 EMBL-EBI Protein of unknown function DUF278 1.0E-83
Pfam IPR000210 EMBL-EBI BTB/POZ domain 2.3E-17
Sequence
Target Sequence 
>C. ELEGANS:180325_AT
gtctcgcatcaagcaggaacaggtgaacccaccaccaccgccacgcgccatcactccact
tcccccggcgacacaccggatcacgatggatgagtataagaagagggagaagaaagacta
ttatcgagatgccaccaaggacgcgagtgtcaagaaggttgtgctctcgctgctcaagga
ctatcccgacatgtggcagaacggtaatcgatttcagactcggaagtggcgagcgctcgg
agttgaagtgtatcagaggactgggcagattgtcggtgtggacgacatgcgaaagatgtt
tatgtcgggaaaaaccgtgctcaaacagaaaatcaccttctgcatccggaatatgaagat
ggaccgcgccgctaccgaggccgacctccagaattgggagtactaccgtcactttctcta
ctaccgtcagacgcttggcaagtttgaggcgaagcttcgcggtgagcagtggatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCTCGCATCAAGCAGGAACAGGTG 480 469 15 Antisense
GACACACCGGATCACGATGGATGAG 85 367 84 Antisense
GGACTATCCCGACATGTGGCAGAAC 46 525 192 Antisense
AGACTCGGAAGTGGCGAGCGCTCGG 160 71 230 Antisense
GAGCGCTCGGAGTTGAAGTGTATCA 545 385 245 Antisense
ATCACCTTCTGCATCCGGAATATGA 49 29 346 Antisense
AATATGAAGATGGACCGCGCCGCTA 610 173 364 Antisense
GAGGCCGACCTCCAGAATTGGGAGT 154 405 391 Antisense
TCTCTACTACCGTCAGACGCTTGGC 526 609 429 Antisense
GACGCTTGGCAAGTTTGAGGCGAAG 666 379 444 Antisense
GAAGCTTCGCGGTGAGCAGTGGATC 622 341 465 Antisense
Sequence Source Affymetrix Proprietary Database