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[180320_at]

Description F35A5.2 /REP_DB=WormBase Gene ID /WP=CE04486 /TR=Q20006 /GB=AAB52642.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 03      6grp_high1_10_10 > 21      4grp_highest_8_8 > 49      4grp_high_8_8 > 41      6grp_highest_nonorm > 42      6grp_total > 32      4grp_total > 54      6grp_highest_sub > De      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 622
1 2 3 4 range
A 943.9
(3)
P 		670.3
(-2.8)
P 		745.9
(-1.2)
P 		661.6
(-3)
P 		282
B 1180.1
(3)
P 		804.3
(-0.6)
P 		755.4
(-1.1)
P 		560.8
(-3)
P 		619
C 1028.2
(3)
P 		807.7
(0.2)
P 		599.9
(-2.5)
P 		558.4
(-3)
P 		470
avr 1050.7
(3) 		760.8
(-0.8) 		700.4
(-1.6) 		593.6
(-3) 		457

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1017
1 2 3 4 5 6 range
A 1066.2
(3)
P 		948.1
(2.1)
P 		1041.3
(2.8)
P 		771.8
(0.8)
P 		594.8
(-0.5)
P 		264.5
(-3)
P 		802
B 1115.7
(2.2)
P 		864.4
(0.7)
P 		1256.9
(3)
P 		826.3
(0.5)
P 		701.9
(-0.3)
P 		239.5
(-3)
P 		1017
C 934
(2.4)
P 		1014
(3)
P 		1009.7
(3)
P 		733.9
(0.8)
P 		565.4
(-0.5)
P 		252.7
(-3)
P 		761
avr 1038.6
(2.5) 		942.2
(1.9) 		1102.6
(3) 		777.3
(0.7) 		620.7
(-0.4) 		252.2
(-3) 		850
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 282
1 2 3 4 range
A 343.7
(3)
P 		145.5
(-1.8)
P 		195
(-0.6)
P 		96.8
(-3)
P 		247
B 376.3
(3)
P 		198.7
(-1.1)
P 		175
(-1.7)
P 		119.5
(-3)
P 		257
C 340.4
(3)
P 		232.2
(0.4)
P 		209.1
(-0.2)
P 		94.2
(-3)
P 		246
avr 353.5
(3) 		192.1
(-0.9) 		193
(-0.9) 		103.5
(-3) 		250

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 324
1 2 3 4 5 6 range
A 196.5
(-0.1)
P 		337.2
(3)
P 		226.8
(0.6)
P 		195.8
(-0.1)
P 		179.5
(-0.5)
P 		64.4
(-3)
P 		273
B 388.2
(3)
P 		287.7
(1)
P 		305.2
(1.3)
P 		218.9
(-0.4)
P 		285.2
(0.9)
P 		89.3
(-3)
P 		299
C 346.2
(3)
P 		309.4
(2.2)
P 		257
(1)
P 		147.6
(-1.6)
P 		183
(-0.7)
P 		84.6
(-3)
P 		262
avr 310.3
(3) 		311.4
(3) 		263
(1.7) 		187.4
(-0.2) 		215.9
(0.5) 		79.4
(-3) 		232
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180320_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18321
Sequence Type Exemplar sequence
Representative Public ID F35A5.2 NCBI
Target Description F35A5.2 /REP_DB=WormBase Gene ID /WP=CE04486 /TR=Q20006 /GB=AAB52642.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076260(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076260 NCBI Caenorhabditis elegans F35A5.2 (F35A5.2) mRNA, complete cds. 11/11 None
SNAP00000032206 ENSEMBL cdna:SNAP chromosome:CEL140:X:3805128:3805602:1 11/11 None
GENEFINDER00000032210 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:3805128:3805602:1 11/11 None
F35A5.2 ENSEMBL cdna:known chromosome:CEL140:X:3805128:3805602:1 gene:F35A5.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:3805128-3805603(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35A5.2
Entrez Gene 185247 Entrez gene
SwissProt Q20006 EMBL-EBI
WormBase CE04486 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB52642 Hypothetical protein F35A5.2 [Caenorhabditis elegans] ref|NP_508661.1| F35A5.2 [Caenorhabditis elegans] 3.0E-58
blast CAE68487 Hypothetical protein CBG14290 [Caenorhabditis briggsae] 7.0E-49
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508661.1 1 5-24
F35A5.2 1 5-24
SNAP00000032206 1 5-24
GENEFINDER00000032210 1 5-24
Sequence
Target Sequence 
>C. ELEGANS:180320_AT
ttattcttgccgtttccatgatacagggtctgactctggttatcaagaaaaaccgcaagt
acaacaatccaccattctgcttcgcctacaacagagagtacaatgataatgatgtgactt
cctcctgctccggttatcaatttgattgctttgattcagctggatcccagattggagacg
tcaagaccttcgccagttcggattgcgaatctatgtcggtgttccgttcgttctccgatg
ccgacttccagaagcgtctcgatgaagccagcaaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTATTCTTGCCGTTTCCATGATACA 441 679 41 Antisense
GATACAGGGTCTGACTCTGGTTATC 241 423 60 Antisense
AACCGCAAGTACAACAATCCACCAT 189 147 91 Antisense
CATTCTGCTTCGCCTACAACAGAGA 160 217 113 Antisense
TGATAATGATGTGACTTCCTCCTGC 395 565 144 Antisense
CTCCTGCTCCGGTTATCAATTTGAT 379 235 162 Antisense
TGCTTTGATTCAGCTGGATCCCAGA 579 579 187 Antisense
GATTGGAGACGTCAAGACCTTCGCC 535 433 210 Antisense
GACCTTCGCCAGTTCGGATTGCGAA 503 383 225 Antisense
TCTATGTCGGTGTTCCGTTCGTTCT 99 613 250 Antisense
AAGCGTCTCGATGAAGCCAGCAAAT 406 147 292 Antisense
Sequence Source Affymetrix Proprietary Database