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[180315_at]

Description F44G3.10 /REP_DB=WormBase Gene ID /WP=CE16041 /TR=O45515 /GB=CAB05514.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 21      4grp_high_8_8 > 42      6grp_total > 32      4grp_total > 53      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 210
1 2 3 4 range
A 225.2
(3)
P 		177.4
(1.3)
P 		202.8
(2.2)
P 		56.7
(-3)
A 		169
B 255.7
(3)
P 		199.1
(1.4)
P 		119.1
(-0.9)
P 		45.3
(-3)
A 		210
C 246.4
(3)
P 		198.6
(1)
P 		178.6
(0.2)
P 		99.5
(-3)
P 		147
avr 242.4
(3) 		191.7
(1.3) 		166.8
(0.4) 		67.2
(-3) 		175

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 555
1 2 3 4 5 6 range
A 403.5
(1)
P 		292.5
(-0.2)
P 		588.5
(3)
P 		357.3
(0.5)
P 		138.3
(-1.9)
P 		33.8
(-3)
A 		555
B 357.3
(1.8)
P 		429.1
(3)
P 		321.5
(1.2)
P 		324.4
(1.2)
P 		144.3
(-1.8)
P 		70.5
(-3)
P 		359
C 326.1
(1.7)
P 		401.7
(3)
P 		342.3
(2)
P 		364.1
(2.4)
P 		116
(-1.8)
P 		42.4
(-3)
P 		359
avr 362.3
(2.1) 		374.4
(2.3) 		417.4
(3) 		348.6
(1.9) 		132.9
(-1.6) 		48.9
(-3) 		369
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 82
(3)
P 		38.5
(-0.5)
P 		53
(0.6)
P 		8.3
(-3)
A 		74
B 81.6
(3)
P 		49.2
(0.3)
P 		27.6
(-1.5)
P 		9.7
(-3)
A 		72
C 81.6
(3)
P 		57.1
(0.7)
P 		62.2
(1.2)
P 		16.8
(-3)
P 		65
avr 81.7
(3) 		48.3
(0.1) 		47.6
(0.1) 		11.6
(-3) 		70

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 135
1 2 3 4 5 6 range
A 74.4
(0.3)
P 		104
(1.8)
P 		128.2
(3)
P 		90.6
(1.1)
P 		41.7
(-1.3)
P 		8.2
(-3)
A 		120
B 124.3
(2)
P 		142.8
(3)
P 		78.1
(-0.3)
P 		85.9
(0.1)
P 		58.6
(-1.3)
P 		26.3
(-3)
P 		117
C 120.9
(2.9)
P 		122.6
(3)
P 		87.1
(1)
P 		73.2
(0.3)
P 		37.5
(-1.7)
P 		14.2
(-3)
P 		108
avr 106.5
(2.1) 		123.1
(3) 		97.8
(1.6) 		83.2
(0.8) 		45.9
(-1.3) 		16.2
(-3) 		107
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180315_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13569
Sequence Type Exemplar sequence
Representative Public ID F44G3.10 NCBI
Target Description F44G3.10 /REP_DB=WormBase Gene ID /WP=CE16041 /TR=O45515 /GB=CAB05514.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074662(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074662 NCBI Caenorhabditis elegans F44G3.10 (F44G3.10) mRNA, complete cds. 11/11 None
SNAP00000003423 ENSEMBL cdna:SNAP chromosome:CEL140:V:16141429:16142199:-1 11/11 None
GENEFINDER00000003428 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:16141429:16142199:-1 11/11 None
F44G3.10 ENSEMBL cdna:known chromosome:CEL140:V:16141429:16142199:-1 gene:F44G3.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16141428-16142199(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F44G3.10
Entrez Gene 185758 Entrez gene
SwissProt O45515 EMBL-EBI
WormBase CE16041 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05514 Hypothetical protein F44G3.10 [Caenorhabditis elegans] ref|NP_507063.1| F44G3.10 [Caenorhabditis elegans] 3.0E-71
blast CAB07339 Hypothetical protein F10A3.1 [Caenorhabditis elegans] ref|NP_507064.1| F10A3.1 [Caenorhabditis elegans] 5.0E-43
blast CAA20971 Hypothetical protein F49H6.13 [Caenorhabditis elegans] emb|CAB04448.1| Hypothetical protein F49H6.13 [Caenorhabditis elegans] ref|NP_507304.1| F49H6.13 [Caenorhabditis elegans] 2.0E-27
Protein Domains
Database ID Description E-Value
Pfam IPR009545 EMBL-EBI Protein of unknown function DUF1164 3.8E-120
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507063.1 4 7-29,49-71,92-114,134-156
F44G3.10 4 7-29,49-71,92-114,134-156
SNAP00000003423 4 7-29,49-71,92-114,134-156
GENEFINDER00000003428 4 7-29,49-71,92-114,134-156
Sequence
Target Sequence 
>C. ELEGANS:180315_AT
ggctgtcaagcgacttttcttcagtgctcttaaccttttaggtggtatttgtatacttat
tggcgttggaacgccagcatggttaacggatgatggaggcagcgttggaatcgttccgtt
ttactcgactgaagttggatggttcgccgctgccagttggatgatgttcatttccctttc
tctggcagttgtagtctcgttgtttactgcaatgttattccgcgatgtcagaagacatgg
atattcttacactcaacgctcaagattcttccttcttgcaatgttcgcactgatggttac
tattttaacagttgttgccgtgattttgattggagtcaacttgccatcttttaacgatta
ttggtacgatgatgcaacactgggatattctgcatgggtcagtgttgctggtgcagtttg
cttttttgtggaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCTGTCAAGCGACTTTTCTTCAGT 333 541 18 Antisense
TTTCTTCAGTGCTCTTAACCTTTTA 142 673 33 Antisense
GGAGGCAGCGTTGGAATCGTTCCGT 653 515 112 Antisense
GAATCGTTCCGTTTTACTCGACTGA 188 327 125 Antisense
TGGATGATGTTCATTTCCCTTTCTC 693 549 175 Antisense
TTCTCTGGCAGTTGTAGTCTCGTTG 551 689 195 Antisense
ACTGCAATGTTATTCCGCGATGTCA 545 97 223 Antisense
ATGGATATTCTTACACTCAACGCTC 613 51 254 Antisense
GCAATGTTCGCACTGATGGTTACTA 441 321 295 Antisense
GAGTCAACTTGCCATCTTTTAACGA 419 399 350 Antisense
GGTGCAGTTTGCTTTTTTGTGGAAT 192 501 427 Antisense
Sequence Source Affymetrix Proprietary Database