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[180311_at]

Description M04G7.1 /REP_DB=WormBase Gene ID /WP=CE17190 /TR=O44179 /GB=AAB88364.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 03      6grp_high1_10_10 > 11      4grp_highest_8_8 > 49      4grp_high_8_8 > 41      6grp_highest_nonorm > 21      6grp_total > 13      4grp_total > 54      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2027
1 2 3 4 range
A 2628.2
(3)
P 		1829.7
(0.6)
P 		1323.4
(-1)
P 		646.4
(-3)
P 		1982
B 2542.7
(3)
P 		1442.2
(-0.5)
P 		1100.1
(-1.5)
P 		638.3
(-3)
P 		1904
C 2628
(3)
P 		1523.2
(-0.3)
P 		972
(-1.9)
P 		600.9
(-3)
P 		2027
avr 2599.6
(3) 		1598.4
(-0) 		1131.8
(-1.5) 		628.5
(-3) 		1971

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1879
1 2 3 4 5 6 range
A 1394.4
(0.1)
P 		2036.7
(3)
P 		1215.4
(-0.7)
P 		1834.6
(2.1)
P 		848.9
(-2.4)
P 		716.7
(-3)
P 		1320
B 1461.9
(3)
P 		1389.3
(2.5)
P 		1385.2
(2.5)
P 		1180.6
(1.2)
P 		1077.5
(0.5)
P 		530.7
(-3)
P 		931
C 2409.3
(3)
P 		1275.1
(-0.7)
P 		1897.8
(1.3)
P 		1062
(-1.4)
P 		1444.9
(-0.1)
P 		558.2
(-3)
P 		1851
avr 1755.2
(3) 		1567
(2) 		1499.5
(1.7) 		1359.1
(0.9) 		1123.8
(-0.3) 		601.9
(-3) 		1153
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 862
1 2 3 4 range
A 956.9
(3)
P 		397.2
(-0.9)
P 		346
(-1.3)
P 		94.6
(-3)
P 		862
B 810.9
(3)
P 		356.2
(-1)
P 		254.9
(-1.9)
P 		136
(-3)
P 		675
C 870
(3)
P 		437.9
(-0.4)
P 		338.8
(-1.1)
P 		101.3
(-3)
P 		769
avr 879.3
(3) 		397.1
(-0.8) 		313.2
(-1.4) 		110.6
(-3) 		769

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 719
1 2 3 4 5 6 range
A 257
(-2.1)
P 		724.4
(3)
P 		264.7
(-2)
P 		465.3
(0.2)
P 		256.2
(-2.1)
P 		174.5
(-3)
P 		550
B 508.7
(3)
P 		462.5
(2.1)
P 		336.4
(-0.3)
P 		312.7
(-0.8)
P 		437.8
(1.6)
P 		197.9
(-3)
P 		311
C 893
(3)
P 		389.1
(-1.3)
P 		483
(-0.5)
P 		213.6
(-2.8)
P 		467.5
(-0.6)
P 		186.9
(-3)
P 		706
avr 552.9
(3) 		525.3
(2.5) 		361.4
(-0.1) 		330.5
(-0.6) 		387.2
(0.3) 		186.4
(-3) 		367
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180311_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10722
Sequence Type Exemplar sequence
Representative Public ID M04G7.1 NCBI
Target Description M04G7.1 /REP_DB=WormBase Gene ID /WP=CE17190 /TR=O44179 /GB=AAB88364.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067532(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067532 NCBI Caenorhabditis elegans M04G7.1 (M04G7.1) mRNA, complete cds. 11/11 None
SNAP00000026149 ENSEMBL cdna:SNAP chromosome:CEL140:IV:455113:457025:1 10/11 None
GENEFINDER00000026153 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:455852:457025:1 11/11 None
M04G7.1 ENSEMBL cdna:known chromosome:CEL140:IV:455726:457052:1 gene:M04G7.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:455725-457025(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl M04G7.1
Entrez Gene 176875 Entrez gene
SwissProt O44179 EMBL-EBI
WormBase CE17190 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB88364 Hypothetical protein M04G7.1 [Caenorhabditis elegans] ref|NP_499933.1| M04G7.1 [Caenorhabditis elegans] 1.0E-156
blast AAB88364 Hypothetical protein M04G7.1 [Caenorhabditis elegans] ref|NP_499933.1| M04G7.1 [Caenorhabditis elegans] 1.0E-131
blast AAB88364 Hypothetical protein M04G7.1 [Caenorhabditis elegans] ref|NP_499933.1| M04G7.1 [Caenorhabditis elegans] 1.0E-110
blast CAE58478 Hypothetical protein CBG01620 [Caenorhabditis briggsae] 1.0E-107
blast CAE58478 Hypothetical protein CBG01620 [Caenorhabditis briggsae] 9.0E-95
blast CAB04039 Hypothetical protein F01D5.1 [Caenorhabditis elegans] ref|NP_496932.1| F01D5.1 [Caenorhabditis elegans] 1.0E-11
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 7.8E-8
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.1E-12
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 0.29
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 7.8E-8
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.1E-12
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 0.29
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 7.8E-8
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.1E-12
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 0.29
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499933.1 1 34-56
M04G7.1 1 34-56
Sequence
Target Sequence 
>C. ELEGANS:180311_AT
tgaatgcttcatgggagcctgcccatcgggcttcgagtgcatcggaaaccaatgttgctc
cgaagccgacgttgttcaacctggaggcgactgtactgattacttggctgattgtaccaa
cgtccaatgtaacgcaatcggaatgcaggatttcgccagagcaaattgtgcaagaacctg
taatatgtgctatagtacagctcctgtgagcccacaatacgcctgtaccgatcttctcac
cgactgcgctaatcgtacggcttcttgccaagacattgactttgttgatatgatggcttt
gtactgcccgagaacttgcagtctctgtttgtgggccgccgccacgaaaattcccaattg
cggaaacacacttccagattgcaccacacgtggaaactactgctccgccacaagcgtctc
cgcctaccagaagcgtgtcggatgcgggaatcagtgtggtttgtgctcccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAATGCTTCATGGGAGCCTGCCCA 579 575 243 Antisense
TCGGGCTTCGAGTGCATCGGAAACC 505 601 268 Antisense
CAACCTGGAGGCGACTGTACTGATT 35 187 319 Antisense
ATAGTACAGCTCCTGTGAGCCCACA 46 21 434 Antisense
CACCGACTGCGCTAATCGTACGGCT 710 199 480 Antisense
AATCGTACGGCTTCTTGCCAAGACA 253 169 493 Antisense
GATGGCTTTGTACTGCCCGAGAACT 215 407 534 Antisense
GAACTTGCAGTCTCTGTTTGTGGGC 169 349 554 Antisense
CCACACGTGGAAACTACTGCTCCGC 660 273 626 Antisense
GCCTACCAGAAGCGTGTCGGATGCG 709 277 664 Antisense
GGAATCAGTGTGGTTTGTGCTCCCC 339 533 689 Antisense
Sequence Source Affymetrix Proprietary Database