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[180306_at]

Description C10G8.4 /REP_DB=WormBase Gene ID /WP=CE08075 /TR=Q94162 /GB=AAB09171.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 93      4grp_total > 03      6grp_total_sub > Hat      6grp_rule_based > S5      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 67
1 2 3 4 range
A 37.6
(0.2)
A 		25.8
(-0.9)
A 		2.5
(-3)
A 		68.9
(3)
A 		66
B 30.4
(0.3)
A 		17.9
(-3)
A 		22.1
(-1.9)
A 		40.6
(3)
A 		23
C 3
(-3)
A 		9
(-2.5)
A 		37.2
(0.1)
A 		69.9
(3)
P 		67
avr 23.7
(-2.1) 		17.6
(-3) 		20.6
(-2.6) 		59.8
(3) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2369
1 2 3 4 5 6 range
A 18.8
(-3)
A 		33
(-3)
A 		25
(-3)
A 		31.5
(-3)
A 		1907.1
(3)
P 		307.1
(-2.1)
P 		1888
B 18.8
(-3)
A 		43.5
(-2.9)
A 		22.8
(-3)
A 		4.7
(-3)
A 		2373.7
(3)
P 		471.3
(-1.8)
P 		2369
C 17.1
(-3)
A 		24
(-3)
A 		17.4
(-3)
A 		36.7
(-2.9)
A 		1922.9
(3)
P 		552.1
(-1.3)
P 		1906
avr 18.2
(-3) 		33.5
(-3) 		21.7
(-3) 		24.3
(-3) 		2067.9
(3) 		443.5
(-1.8) 		2050
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 13.7
(3)
A 		5.6
(-0.7)
A 		0.6
(-3)
A 		10.1
(1.4)
A 		13
B 9.7
(3)
A 		4.4
(-3)
A 		5.1
(-2.2)
A 		8.6
(1.8)
A 		5
C 1
(-3)
A 		2.6
(-2.2)
A 		13
(3)
A 		11.8
(2.4)
P 		12
avr 8.1
(0.9) 		4.2
(-3) 		6.2
(-1) 		10.2
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 963
1 2 3 4 5 6 range
A 3.5
(-3)
A 		11.7
(-2.9)
A 		5.4
(-3)
A 		8
(-3)
A 		575.5
(3)
P 		74.8
(-2.3)
P 		572
B 6.5
(-3)
A 		14.5
(-2.9)
A 		5.5
(-3)
A 		1.2
(-3)
A 		964.4
(3)
P 		175.7
(-1.9)
P 		963
C 6.3
(-3)
A 		7.3
(-3)
A 		4.4
(-3)
A 		7.4
(-3)
A 		622.2
(3)
P 		184.8
(-1.2)
P 		618
avr 5.4
(-3) 		11.2
(-2.9) 		5.1
(-3) 		5.5
(-3) 		720.7
(3) 		145.1
(-1.8) 		716
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180306_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12094
Sequence Type Exemplar sequence
Representative Public ID C10G8.4 NCBI
Target Description C10G8.4 /REP_DB=WormBase Gene ID /WP=CE08075 /TR=Q94162 /GB=AAB09171.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072015(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072015 NCBI Caenorhabditis elegans C10G8.4 (C10G8.4) mRNA, complete cds. 11/11 None
SNAP00000038001 ENSEMBL cdna:SNAP chromosome:CEL140:V:5311510:5312092:1 9/11 None
C10G8.4 ENSEMBL cdna:known chromosome:CEL140:V:5311747:5312092:1 gene:C10G8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:5311746-5312092(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C10G8.4
Entrez Gene 182503 Entrez gene
SwissProt Q94162 EMBL-EBI
WormBase CE08075 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB09171 Hypothetical protein C10G8.4 [Caenorhabditis elegans] ref|NP_504416.1| C10G8.4 [Caenorhabditis elegans] 1.0E-46
blast AAB09171 Hypothetical protein C10G8.4 [Caenorhabditis elegans] ref|NP_504416.1| C10G8.4 [Caenorhabditis elegans] 3.0E-37
blast CAE62644 Hypothetical protein CBG06778 [Caenorhabditis briggsae] 3.0E-23
blast AAB65990 Hypothetical protein B0238.12 [Caenorhabditis elegans] ref|NP_504409.1| B0238.12 [Caenorhabditis elegans] 3.0E-20
Protein Domains
Database ID Description E-Value
Pfam IPR002919 EMBL-EBI Trypsin inhibitor-like, cysteine-rich TIL region 6.4E-25
Pfam IPR002919 EMBL-EBI Trypsin inhibitor-like, cysteine-rich TIL region 6.4E-25
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504416.1 1 13-35
C10G8.4 1 13-35
SNAP00000038001 1 15-37
Sequence
Target Sequence 
>C. ELEGANS:180306_AT
atgctgccgatttcaacagtcctatttcattctgaaatttttgttaaaatgaagttcctg
atcattctccttgtcgctattgtcaccataacagcagctcaaggaagataccaaagatgt
ccatcaaatgaagaattccgatcatgtggaacggcttgtgaaccaacttgccagaatcct
aatccacaagtttgcactcttcaatgcatcttgaacgtttgtcaatgttcccaaggattt
gtgagagggccaaatggatgtgttcct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCTGCCGATTTCAACAGTCCTAT 122 43 13 Antisense
TCAACAGTCCTATTTCATTCTGAAA 599 629 25 Antisense
CCTTGTCGCTATTGTCACCATAACA 584 269 81 Antisense
TTGTCACCATAACAGCAGCTCAAGG 414 705 92 Antisense
GAATTCCGATCATGTGGAACGGCTT 459 323 145 Antisense
GGAACGGCTTGTGAACCAACTTGCC 270 527 160 Antisense
CCAACTTGCCAGAATCCTAATCCAC 378 275 175 Antisense
CCTAATCCACAAGTTTGCACTCTTC 449 267 190 Antisense
GTTTGCACTCTTCAATGCATCTTGA 524 451 202 Antisense
AACGTTTGTCAATGTTCCCAAGGAT 504 135 226 Antisense
GAGAGGGCCAAATGGATGTGTTCCT 238 393 255 Antisense
Sequence Source Affymetrix Proprietary Database