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[180305_at]

Description F22E5.8 /REP_DB=WormBase Gene ID /WP=CE27994 /TR=O16706 /GB=AAB66174.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 88      4grp_total > 40      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 36
1 2 3 4 range
A 2.9
(-3)
A 		23.7
(0.5)
A 		38.4
(3)
M 		7.8
(-2.2)
A 		36
B 12.4
(0.9)
A 		4.7
(-3)
A 		5.6
(-2.6)
A 		16.7
(3)
A 		12
C 2.8
(-2.1)
A 		5.2
(3)
A 		2.4
(-3)
A 		3.7
(-0.2)
A 		3
avr 6
(-3) 		11.2
(0.3) 		15.5
(3) 		9.4
(-0.9) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 47
1 2 3 4 5 6 range
A 44.8
(2.5)
A 		5.3
(-3)
A 		41.4
(2)
P 		27.2
(0)
A 		43.2
(2.3)
P 		48.4
(3)
A 		43
B 2.5
(-3)
A 		18.3
(-0.3)
P 		36.7
(2.7)
P 		29.8
(1.6)
A 		25.8
(0.9)
A 		38.2
(3)
A 		36
C 1
(-3)
A 		35.7
(3)
P 		5.2
(-2.3)
A 		1.8
(-2.9)
A 		16.9
(-0.3)
A 		32.2
(2.4)
P 		35
avr 16.1
(-3) 		19.8
(-2.1) 		27.8
(-0) 		19.6
(-2.1) 		28.6
(0.2) 		39.6
(3) 		24
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 1.1
(-3)
A 		5.1
(-0.3)
A 		10
(3)
M 		1.1
(-3)
A 		9
B 3.9
(3)
A 		1.2
(-3)
A 		1.3
(-2.8)
A 		3.6
(2.3)
A 		3
C 0.9
(-1)
A 		1.5
(3)
A 		0.8
(-1.7)
A 		0.6
(-3)
A 		1
avr 2
(-2.5) 		2.6
(-0.8) 		4
(3) 		1.8
(-3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 8.3
(0.5)
A 		1.9
(-3)
A 		9
(0.8)
P 		6.9
(-0.3)
A 		13
(3)
P 		11.8
(2.4)
A 		11
B 0.9
(-3)
A 		6.1
(-0.7)
P 		8.9
(0.6)
P 		7.9
(0.2)
A 		10.5
(1.3)
A 		14.2
(3)
A 		13
C 0.4
(-3)
A 		10.9
(3)
P 		1.3
(-2.5)
A 		0.4
(-3)
A 		5.5
(-0.1)
A 		10.8
(2.9)
P 		11
avr 3.2
(-3) 		6.3
(-1) 		6.4
(-0.9) 		5.1
(-1.7) 		9.7
(1.3) 		12.3
(3) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180305_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5882
Sequence Type Exemplar sequence
Representative Public ID F22E5.8 NCBI
Target Description F22E5.8 /REP_DB=WormBase Gene ID /WP=CE27994 /TR=O16706 /GB=AAB66174.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061914(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061914 NCBI Caenorhabditis elegans F22E5.8 (F22E5.8) mRNA, complete cds. 11/11 None
SNAP00000023604 ENSEMBL cdna:SNAP chromosome:CEL140:II:2633335:2640623:1 10/11 None
GENEFINDER00000023631 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2640082:2642885:1 11/11 None
F22E5.8 ENSEMBL cdna:known chromosome:CEL140:II:2640082:2641898:1 gene:F22E5.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2640080-2640533(+) 100.0 100.0
chrII:3224187-3224637(-) 90.62 90.62
Public Domain and Genome References
Gene Title
Ensembl F22E5.8
Entrez Gene 173610 Entrez gene
SwissProt O16706 EMBL-EBI
WormBase CE27994 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6813 potassium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
8076 voltage-gated potassium channel complex inferred from electronic annotation QuickGO AmiGO
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5249 voltage-gated potassium channel activity inferred from electronic annotation QuickGO AmiGO
5515 protein binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_494315 F22E5.8 [Caenorhabditis elegans] gb|AAB66174.2| Hypothetical protein F22E5.8 [Caenorhabditis elegans] 1.0E-133
blast AAB37955 Hypothetical protein ZC239.15 [Caenorhabditis elegans] ref|NP_494481.1| ZC239.15 [Caenorhabditis elegans] 1.0E-92
blast NP_494315 F22E5.8 [Caenorhabditis elegans] gb|AAB66174.2| Hypothetical protein F22E5.8 [Caenorhabditis elegans] 1.0E-87
blast AAB37955 Hypothetical protein ZC239.15 [Caenorhabditis elegans] ref|NP_494481.1| ZC239.15 [Caenorhabditis elegans] 3.0E-76
blast T32066 hypothetical protein F22E5.7 - Caenorhabditis elegans 3.0E-66
blast AAB37956 Hypothetical protein ZC239.14 [Caenorhabditis elegans] ref|NP_494479.1| ZC239.14 [Caenorhabditis elegans] 5.0E-58
blast T32063 hypothetical protein F22E5.10 - Caenorhabditis elegans 4.0E-46
Protein Domains
Database ID Description E-Value
Pfam IPR003131 EMBL-EBI K+ channel tetramerisation 5.7E-45
Pfam IPR003131 EMBL-EBI K+ channel tetramerisation 4.2E-27
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000023604 4 5-24,63-85,105-127,148-167
Sequence
Target Sequence 
>C. ELEGANS:180305_AT
agtgaaactgtacaactggacgttggtggaactatattcaaaacttcaaaatctacatta
acaaagttcaatggatttttaaaaattatgctggagagtgacattgggctaaaaatcgac
gaatctgggagtatttttattgatcgttccccgaaacattttgacttgatactgaacttc
atgcgagatggtgacgtggttctacccagttgtgaactcacagtgaaggagctgctagca
gaagctcaattctatttgctcgatgaactaattgagttgtgtagtttaaagattgagcct
gttcaagctccaaagataaaacttcggtttattgaaaatgattcacagctgcttcaaatt
cttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTGAAACTGTACAACTGGACGTTG 493 57 16 Antisense
TCGTTCCCCGAAACATTTTGACTTG 280 599 159 Antisense
ATACTGAACTTCATGCGAGATGGTG 657 21 184 Antisense
GCGAGATGGTGACGTGGTTCTACCC 623 287 198 Antisense
TTCTACCCAGTTGTGAACTCACAGT 79 689 215 Antisense
AACTCACAGTGAAGGAGCTGCTAGC 350 141 230 Antisense
ATTCTATTTGCTCGATGAACTAATT 606 9 264 Antisense
GAGTTGTGTAGTTTAAAGATTGAGC 532 395 289 Antisense
AAAGATTGAGCCTGTTCAAGCTCCA 524 121 303 Antisense
GAAAATGATTCACAGCTGCTTCAAA 9 355 349 Antisense
GATTCACAGCTGCTTCAAATTCTTG 433 431 355 Antisense
Sequence Source Affymetrix Proprietary Database