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[180304_at]

Description C01F4.2 /REP_DB=WormBase Gene ID /WP=CE27576 /TR=O01443 /GB=AAB52435.2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 24      6grp_total > 02      4grp_total > 21      6grp_high1_sub > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 169
1 2 3 4 range
A 120.6
(-2)
P 		122.1
(-1.9)
A 		102.6
(-3)
A 		208.3
(3)
P 		106
B 122.6
(1)
P 		58.1
(-2.4)
A 		45.8
(-3)
A 		160.6
(3)
P 		115
C 117
(3)
P 		78.7
(0.1)
P 		39.1
(-3)
A 		111.8
(2.6)
P 		78
avr 120.1
(0.5) 		86.3
(-1.5) 		62.5
(-3) 		160.2
(3) 		98

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 59.4
(-3)
A 		102.5
(3)
P 		70.5
(-1.5)
P 		102.3
(3)
P 		85.9
(0.7)
P 		64
(-2.4)
A 		43
B 85.3
(2.7)
P 		87.2
(3)
P 		74.9
(1)
P 		50.8
(-3)
P 		63.3
(-0.9)
P 		56.1
(-2.1)
P 		36
C 89.3
(0.7)
P 		114.9
(3)
P 		46.7
(-3)
A 		65.3
(-1.4)
P 		85.1
(0.4)
P 		63.4
(-1.5)
P 		68
avr 78
(-0.5) 		101.5
(3) 		64
(-2.6) 		72.8
(-1.3) 		78.1
(-0.5) 		61.2
(-3) 		40
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 33
1 2 3 4 range
A 43.9
(3)
P 		26.5
(-3)
A 		26.8
(-2.9)
A 		30.5
(-1.6)
P 		17
B 39.1
(3)
P 		14.4
(-2.2)
A 		10.6
(-3)
A 		34.2
(2)
P 		29
C 38.7
(3)
P 		22.6
(-0.8)
P 		13.6
(-3)
A 		18.8
(-1.8)
P 		25
avr 40.6
(3) 		21.2
(-1.9) 		17
(-3) 		27.8
(-0.3) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 10.9
(-3)
A 		36.4
(3)
P 		15.4
(-1.9)
P 		25.9
(0.5)
P 		25.9
(0.5)
P 		15.6
(-1.9)
A 		26
B 29.7
(3)
P 		29
(2.7)
P 		18.2
(-1.3)
P 		13.5
(-3)
P 		25.7
(1.5)
P 		20.9
(-0.3)
P 		16
C 33.1
(2.5)
P 		35.1
(3)
P 		11.9
(-3)
A 		13.1
(-2.7)
P 		27.5
(1)
P 		21.2
(-0.6)
P 		23
avr 24.6
(0.1) 		33.5
(3) 		15.2
(-3) 		17.5
(-2.2) 		26.4
(0.7) 		19.2
(-1.7) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180304_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1454
Sequence Type Exemplar sequence
Representative Public ID C01F4.2 NCBI
Target Description C01F4.2 /REP_DB=WormBase Gene ID /WP=CE27576 /TR=O01443 /GB=AAB52435.2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059064(11), NM_059065(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059064 NCBI Caenorhabditis elegans C01F4.2a (C01F4.2) mRNA, complete cds. 11/11 A 
NM_059065 NCBI Caenorhabditis elegans C01F4.2b (C01F4.2) mRNA, complete cds. 11/11 A 
GENEFINDER00000038083 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4850995:4867826:-1 11/11 None
C01F4.2a ENSEMBL cdna:known chromosome:CEL140:I:4850673:4867823:-1 gene:C01F4.2 11/11 A 
C01F4.2b ENSEMBL cdna:known chromosome:CEL140:I:4850976:4867826:-1 gene:C01F4.2 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000038082 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4853878-4856769(-) 100.0 100.0
Public Domain and Genome References
Ensembl C01F4.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB52434 Hypothetical protein C01F4.2a [Caenorhabditis elegans] ref|NP_491465.2| C01F4.2a [Caenorhabditis elegans] 0.0
blast CAE60348 Hypothetical protein CBG03944 [Caenorhabditis briggsae] 0.0
blast AAO38678 Hypothetical protein C01F4.2b [Caenorhabditis elegans] ref|NP_491466.2| C01F4.2b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000198 EMBL-EBI RhoGAP domain 1.3E-58
Pfam IPR000198 EMBL-EBI RhoGAP domain 1.3E-58
Pfam IPR000198 EMBL-EBI RhoGAP domain 5.1E-30
Sequence
Target Sequence 
>C. ELEGANS:180304_AT
ccgtcttcgcgattcacgagattcgtcggatttcaacgattcgcatgacacgctagacat
cgccggaacgagcgcgcacggcgtcgccgattcgtcgtctcatcaccatcacaactactc
gatgacggcacctgtttctccgaatgttccgctttcagcgagcagttcgcattccgaggg
atggaaatactcatcgcagaagcttctatggaagttgaaaccaaaatatgcatattcaca
tgcaagttcaacatcttcatcaacagattctgcatggaaatcattggacagtatgacatg
gcgatcggtagatggtgccgaagtggtgttacgtggtgctcgactagaaaacctatcaga
aatcgaacgatctgctcttcaactcctcgccgctcaacgtctcacaaaaatgct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCGTCTTCGCGATTCACGAGATTCG 88 265 45 Antisense
GATTCGTCGGATTTCAACGATTCGC 140 433 64 Antisense
AACGATTCGCATGACACGCTAGACA 46 145 79 Antisense
GCTAGACATCGCCGGAACGAGCGCG 662 301 96 Antisense
ATCACAACTACTCGATGACGGCACC 704 25 152 Antisense
CTCCGAATGTTCCGCTTTCAGCGAG 650 233 182 Antisense
TCAGCGAGCAGTTCGCATTCCGAGG 16 623 199 Antisense
AAATACTCATCGCAGAAGCTTCTAT 48 115 229 Antisense
GATGGTGCCGAAGTGGTGTTACGTG 115 405 355 Antisense
GGTGTTACGTGGTGCTCGACTAGAA 328 499 369 Antisense
CCGCTCAACGTCTCACAAAAATGCT 452 263 434 Antisense
Sequence Source Affymetrix Proprietary Database