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[180298_s_at]

Description K02E11.1 /REP_DB=WormBase Gene ID /WP=CE11644 /TR=Q21145 /GB=CAB01223.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 40      6grp_cgc > 69      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 32
1 2 3 4 range
A 2.6
(-3)
A 		3.6
(-2.8)
A 		34.2
(3)
A 		17.6
(-0.2)
A 		32
B 7.6
(-2.5)
A 		6.5
(-3)
A 		20.3
(3)
A 		10.9
(-1.1)
A 		14
C 3.9
(-3)
A 		21.4
(3)
A 		6
(-2.3)
A 		10.2
(-0.8)
A 		18
avr 4.7
(-3) 		10.5
(-0.8) 		20.2
(3) 		12.9
(0.2) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 94
1 2 3 4 5 6 range
A 5
(-2.8)
A 		1.8
(-3)
A 		6.9
(-2.6)
A 		24.6
(-1.2)
A 		78.7
(3)
A 		73
(2.6)
A 		77
B 6.1
(-3)
A 		14.3
(-2.4)
A 		32.9
(-1.2)
A 		32.4
(-1.2)
A 		88.7
(2.5)
M 		95.4
(3)
P 		89
C 24.1
(-2)
A 		44.6
(-0.5)
A 		12.9
(-2.8)
A 		9.6
(-3)
A 		28.6
(-1.6)
A 		93.6
(3)
P 		84
avr 11.7
(-3) 		20.2
(-2.3) 		17.6
(-2.5) 		22.2
(-2.2) 		65.3
(1.3) 		87.3
(3) 		76
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 0.9
(-2.9)
A 		0.8
(-3)
A 		9
(3)
A 		2.6
(-1.7)
A 		8
B 2.4
(-1.5)
A 		1.6
(-3)
A 		4.7
(3)
A 		2.3
(-1.6)
A 		3
C 1.3
(-3)
A 		6.2
(3)
A 		2.1
(-2)
A 		1.7
(-2.5)
A 		5
avr 1.5
(-3) 		2.9
(-0.8) 		5.3
(3) 		2.2
(-1.9) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 35
1 2 3 4 5 6 range
A 0.9
(-2.9)
A 		0.6
(-3)
A 		1.5
(-2.8)
A 		6.2
(-1.5)
A 		23.7
(3)
A 		17.8
(1.5)
A 		23
B 2.1
(-3)
A 		4.8
(-2.5)
A 		8
(-2)
A 		8.6
(-1.8)
A 		36
(3)
M 		35.6
(2.9)
P 		34
C 8.9
(-1.6)
A 		13.6
(-0.6)
A 		3.3
(-2.7)
A 		1.9
(-3)
A 		9.2
(-1.5)
A 		31.3
(3)
P 		29
avr 4
(-3) 		6.3
(-2.4) 		4.3
(-2.9) 		5.6
(-2.6) 		23
(1.7) 		28.2
(3) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180298_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13469
Sequence Type Exemplar sequence
Representative Public ID K02E11.1 NCBI
Target Description K02E11.1 /REP_DB=WormBase Gene ID /WP=CE11644 /TR=Q21145 /GB=CAB01223.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074120(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074120 NCBI Caenorhabditis elegans Equilibrative Nucleoside Transporter family member (ent-3) (ent-3) mRNA, complete cds. 11/11 None
SNAP00000039256 ENSEMBL cdna:SNAP chromosome:CEL140:V:14234909:14238590:-1 11/11 None
GENEFINDER00000039272 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14234909:14242303:-1 11/11 None
K02E11.1 ENSEMBL cdna:known chromosome:CEL140:V:14234909:14238640:-1 gene:K02E11.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14234908-14239294(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ent-3
Ensembl K02E11.1
Entrez Gene 179926 Entrez gene
SwissProt Q21145 EMBL-EBI
WormBase CE37978 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.22131.1.S1_S_AT similar to solute carrier family 29 (nucleoside transporters), member 3 cfa
CHICKEN:GGAAFFX.2834.1.S1_S_AT similar to solute carrier family 29 (nucleoside transporters), member 3; equilibrative nucleoside transporter 3 gga
HG-U133_PLUS_2:219344_AT solute carrier family 29 (nucleoside transporters), member 3 hs
HG-U133A:219344_AT solute carrier family 29 (nucleoside transporters), member 3 hs
HG-U133A_2:219344_AT solute carrier family 29 (nucleoside transporters), member 3 hs
U133_X3P:G8922906_3P_AT solute carrier family 29 (nucleoside transporters), member 3 hs
HG-U95E:76604_AT solute carrier family 29 (nucleoside transporters), member 3 hs
MG-U74BV2:110515_AT solute carrier family 29 (nucleoside transporters), member 3 mm
MOE430A:1451013_AT solute carrier family 29 (nucleoside transporters), member 3 mm
MOUSE430A_2:1451013_AT solute carrier family 29 (nucleoside transporters), member 3 mm
MOUSE430_2:1451013_AT solute carrier family 29 (nucleoside transporters), member 3 mm
MOE430A:1455731_AT solute carrier family 29 (nucleoside transporters), member 3 mm
MOUSE430A_2:1455731_AT solute carrier family 29 (nucleoside transporters), member 3 mm
MOUSE430_2:1455731_AT solute carrier family 29 (nucleoside transporters), member 3 mm
RG-U34B:RC_AI011311_AT solute carrier family 29 (nucleoside transporters), member 3 rn
RG-U34B:RC_AI008183_AT solute carrier family 29 (nucleoside transporters), member 3 rn
RAE230A:1374200_AT solute carrier family 29 (nucleoside transporters), member 3 rn
RAT230_2:1374200_AT solute carrier family 29 (nucleoside transporters), member 3 rn
RAE230A:1374221_AT Solute carrier family 29 (nucleoside transporters), member 3 rn
RAT230_2:1374221_AT Solute carrier family 29 (nucleoside transporters), member 3 rn
RG-U34C:RC_AI136912_AT Solute carrier family 29 (nucleoside transporters), member 3 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5337 nucleoside transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01223 Hypothetical protein K02E11.1 [Caenorhabditis elegans] ref|NP_506521.2| Equilibrative Nucleoside Transporter family member (ent-3) [Caenorhabditis elegans] 0.0
blast CAE60999 Hypothetical protein CBG04736 [Caenorhabditis briggsae] 0.0
blast CAE64143 Hypothetical protein CBG08759 [Caenorhabditis briggsae] 1.0E-151
Protein Domains
Database ID Description E-Value
Pfam IPR002259 EMBL-EBI Delayed-early response protein/equilibrative nucleoside transporter 1.0E-126
Pfam IPR002259 EMBL-EBI Delayed-early response protein/equilibrative nucleoside transporter 1.0E-126
Pfam IPR002259 EMBL-EBI Delayed-early response protein/equilibrative nucleoside transporter 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506521.2 11 319-341,373-390,402-420,425-447,460-482,497-516,556-578,598-620,627-645,660-682,702-724
K02E11.1 11 319-341,373-390,402-420,425-447,460-482,497-516,556-578,598-620,627-645,660-682,702-724
SNAP00000039256 11 278-300,332-349,361-379,384-406,419-441,456-475,515-537,557-579,586-604,619-641,661-683
GENEFINDER00000039272 11 366-388,420-437,449-467,472-494,507-529,544-563,603-625,645-667,674-692,707-729,749-771
Sequence
Target Sequence 
>C. ELEGANS:180298_S_AT
tttttcgtcacgctggcgttgttcccgaatgtttgtatgtacgttaaggatgcgaaaaaa
ggagaattgcatagttttgtggttccagaaaaatatttcatggatgttgtcacattcctc
aacttcaacttgttcgcttttcttggaagtttgatggcaaactggattcgattcccagga
cccaacactgtttggatttgtgtagctgctcgcttctggttcatgttctacttcccggct
gccaactatcatccaatggactttccacgtgcctatccggttctcttcgaatccacgtgg
ctttttgcattcaacatttgcattttcgcactgaccagtggatacttgagctctctgatc
atgatgtatgcaccaagatcacacgaagatccgaaaattcaaagaatggctggcatgatt
gcctcgttctttttgatattc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTTTCGTCACGCTGGCGTTGTTCC 663 673 1912 Antisense
GGCGTTGTTCCCGAATGTTTGTATG 73 541 1926 Antisense
TTGTCACATTCCTCAACTTCAACTT 185 707 2018 Antisense
AACTTCAACTTGTTCGCTTTTCTTG 384 139 2032 Antisense
TGGATTTGTGTAGCTGCTCGCTTCT 332 551 2104 Antisense
GGCTGCCAACTATCATCCAATGGAC 657 539 2148 Antisense
TCCACGTGGCTTTTTGCATTCAACA 394 589 2203 Antisense
ACATTTGCATTTTCGCACTGACCAG 678 105 2225 Antisense
GTGGATACTTGAGCTCTCTGATCAT 31 491 2249 Antisense
GAATGGCTGGCATGATTGCCTCGTT 244 333 2315 Antisense
GATTGCCTCGTTCTTTTTGATATTC 119 435 2328 Antisense
Sequence Source Affymetrix Proprietary Database