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[180286_at]

Description W03D8.5 /REP_DB=WormBase Gene ID /WP=CE18325 /TR=O44999 /GB=AAK21491.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 20      4grp_total > 49      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 2.4
(-3)
A 		81.1
(3)
A 		78
(2.8)
A 		11.4
(-2.3)
A 		79
B 2
(-3)
A 		101.1
(3)
P 		99.2
(2.9)
P 		3.9
(-2.9)
A 		99
C 11.7
(-3)
A 		64.7
(1.1)
A 		89.2
(3)
P 		19
(-2.4)
A 		78
avr 5.4
(-3) 		82.3
(2.5) 		88.8
(3) 		11.4
(-2.6) 		83

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 3.5
(-3)
A 		39.3
(0.7)
A 		61.5
(3)
A 		60.3
(2.9)
A 		25.2
(-0.8)
A 		7.9
(-2.5)
A 		58
B 3.7
(-3)
A 		44.2
(3)
A 		39.3
(2.3)
A 		19.3
(-0.7)
A 		24.5
(0.1)
A 		18.9
(-0.7)
A 		41
C 14.7
(-2.2)
A 		68.3
(3)
A 		33.7
(-0.3)
A 		25.9
(-1.1)
A 		33.1
(-0.4)
A 		6.2
(-3)
A 		62
avr 7.3
(-3) 		50.6
(3) 		44.8
(2.2) 		35.2
(0.9) 		27.6
(-0.2) 		11
(-2.5) 		43
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 31
1 2 3 4 range
A 0.9
(-3)
A 		17.6
(2.1)
A 		20.4
(3)
A 		1.7
(-2.8)
A 		20
B 0.6
(-3)
A 		25
(3)
P 		23
(2.5)
P 		0.8
(-3)
A 		24
C 3.9
(-2.8)
A 		18.6
(0.3)
A 		31.1
(3)
P 		3.2
(-3)
A 		28
avr 1.8
(-3) 		20.4
(1.9) 		24.8
(3) 		1.9
(-3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 0.6
(-3)
A 		14
(2.5)
A 		13.4
(2.2)
A 		15.3
(3)
A 		7.6
(-0.1)
A 		1.9
(-2.5)
A 		15
B 1.3
(-3)
A 		14.7
(3)
A 		9.5
(0.7)
A 		5.1
(-1.3)
A 		9.9
(0.9)
A 		7
(-0.4)
A 		13
C 5.4
(-1.9)
A 		20.8
(3)
A 		8.6
(-0.9)
A 		5.2
(-2)
A 		10.7
(-0.2)
A 		2.1
(-3)
A 		19
avr 2.4
(-3) 		16.5
(3) 		10.5
(0.4) 		8.5
(-0.4) 		9.4
(-0) 		3.7
(-2.4) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180286_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.114
Sequence Type Exemplar sequence
Representative Public ID W03D8.5 NCBI
Target Description W03D8.5 /REP_DB=WormBase Gene ID /WP=CE18325 /TR=O44999 /GB=AAK21491.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058650(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058650 NCBI Caenorhabditis elegans W03D8.5 (W03D8.5) mRNA, complete cds. 11/11 None
SNAP00000016960 ENSEMBL cdna:SNAP chromosome:CEL140:I:2816485:2820689:1 9/11 None
GENEFINDER00000016964 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2818794:2820689:1 10/11 None
W03D8.5 ENSEMBL cdna:known chromosome:CEL140:I:2816759:2820023:1 gene:W03D8.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2816484-2820023(+) 82.25 100.0
Public Domain and Genome References
Gene Title
Ensembl W03D8.5
Entrez Gene 171847 Entrez gene
SwissProt O44999 EMBL-EBI
WormBase CE37996 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_491051 W03D8.5 [Caenorhabditis elegans] gb|AAK21491.2| Hypothetical protein W03D8.5 [Caenorhabditis elegans] 1.0E-149
blast NP_491051 W03D8.5 [Caenorhabditis elegans] gb|AAK21491.2| Hypothetical protein W03D8.5 [Caenorhabditis elegans] 1.0E-128
blast CAE60484 Hypothetical protein CBG04098 [Caenorhabditis briggsae] 1.0E-113
blast CAE60484 Hypothetical protein CBG04098 [Caenorhabditis briggsae] 1.0E-76
blast NP_491051 W03D8.5 [Caenorhabditis elegans] gb|AAK21491.2| Hypothetical protein W03D8.5 [Caenorhabditis elegans] 2.0E-73
blast CAE60484 Hypothetical protein CBG04098 [Caenorhabditis briggsae] 3.0E-70
blast CAE60485 Hypothetical protein CBG04099 [Caenorhabditis briggsae] 4.0E-38
Protein Domains
Database ID Description E-Value
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 5.1E-18
Pfam IPR000535 EMBL-EBI Major sperm protein (MSP) domain 5.1E-18
Sequence
Target Sequence 
>C. ELEGANS:180286_AT
gattcggttcatattccagtcgacggtgaaaagctaacagtacccaaggcgttctatcac
tattcggaaaatgaaatgtctgccggtggaagtttcggtcttccagacgagacccgcctg
ttagatactgacggaaaatttgtgaataatcgaaaagaaatcaacggaatttatttgcca
ctgccaaatatggaaccaattaatctacactttgtgaatcaaaaagttttcgaaaaagga
atcgacacgagagcacacgaagaatcaccggaaagtgtgctccacagagctaaaatgatt
tgcttaaccggcagcgagttcgaatgtgagcgacaacttctaaaatatcatcgagccaag
agtgaagccttgcgaagagatcgaatgccacttcatgag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCGGTTCATATTCCAGTCGACG 258 431 784 Antisense
AAGCTAACAGTACCCAAGGCGTTCT 262 141 814 Antisense
CCAAGGCGTTCTATCACTATTCGGA 181 277 827 Antisense
GTCTGCCGGTGGAAGTTTCGGTCTT 223 469 861 Antisense
TTTCGGTCTTCCAGACGAGACCCGC 555 669 876 Antisense
AGACCCGCCTGTTAGATACTGACGG 163 69 893 Antisense
AACGGAATTTATTTGCCACTGCCAA 588 141 946 Antisense
GAGAGCACACGAAGAATCACCGGAA 40 395 1032 Antisense
ATCACCGGAAAGTGTGCTCCACAGA 281 29 1047 Antisense
TTGCTTAACCGGCAGCGAGTTCGAA 626 693 1083 Antisense
AAGAGATCGAATGCCACTTCATGAG 547 153 1158 Antisense
Sequence Source Affymetrix Proprietary Database