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[180284_at]

Description H32K16.1 /REP_DB=WormBase Gene ID /WP=CE16186 /TR=O62286 /GB=CAB10025.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 87      6grp_high1_10_10 > 78      4grp_highest_8_8 > 54      4grp_high_8_8 > 10      6grp_highest_nonorm > 91      6grp_total > 68      4grp_total > 22      6grp_highest_sub > Da      6grp_high1_sub > Cu      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 222
1 2 3 4 range
A 190
(2)
P 		92.6
(-2)
P 		69
(-3)
P 		214.4
(3)
P 		145
B 291.3
(3)
P 		81
(-2.9)
P 		75.8
(-3)
P 		199.3
(0.4)
P 		216
C 169.7
(0.4)
P 		82.8
(-2.9)
P 		78.9
(-3)
P 		240.5
(3)
P 		162
avr 217
(3) 		85.5
(-2.5) 		74.6
(-3) 		218.1
(3) 		144

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 213
1 2 3 4 5 6 range
A 184.8
(0.3)
P 		92
(-3)
P 		128.7
(-1.7)
P 		177.1
(0)
P 		178.6
(0.1)
P 		260.2
(3)
P 		168
B 142
(-1.2)
P 		151.2
(-1)
P 		79.7
(-3)
P 		183.5
(-0.1)
P 		132.8
(-1.5)
P 		293
(3)
P 		213
C 171.6
(0.7)
P 		151.2
(-1.2)
P 		132.8
(-3)
P 		191.5
(2.7)
P 		161.5
(-0.2)
P 		194.9
(3)
P 		62
avr 166.1
(-0.7) 		131.5
(-2.2) 		113.7
(-3) 		184
(0.1) 		157.6
(-1.1) 		249.4
(3) 		136
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 69.2
(3)
P 		20.1
(-2.8)
P 		18
(-3)
P 		31.4
(-1.4)
P 		51
B 92.9
(3)
P 		20
(-2.8)
P 		17.6
(-3)
P 		42.5
(-1)
P 		75
C 56.2
(3)
P 		23.8
(-3)
P 		27.5
(-2.3)
P 		40.5
(0.1)
P 		32
avr 72.8
(3) 		21.3
(-3) 		21
(-3) 		38.1
(-1) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 90
1 2 3 4 5 6 range
A 34.1
(-2)
P 		32.7
(-2.2)
P 		28
(-3)
P 		44.9
(-0.1)
P 		53.9
(1.4)
P 		63.4
(3)
P 		35
B 49.4
(-1)
P 		50.3
(-0.9)
P 		19.4
(-3)
P 		48.6
(-1.1)
P 		54
(-0.7)
P 		109.3
(3)
P 		90
C 63.6
(2.7)
P 		46.2
(-0.6)
P 		33.8
(-3)
P 		38.5
(-2.1)
P 		52.2
(0.5)
P 		65.3
(3)
P 		32
avr 49
(-0.5) 		43.1
(-1.2) 		27.1
(-3) 		44
(-1.1) 		53.4
(0) 		79.3
(3) 		52
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180284_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2386
Sequence Type Exemplar sequence
Representative Public ID H32K16.1 NCBI
Target Description H32K16.1 /REP_DB=WormBase Gene ID /WP=CE16186 /TR=O62286 /GB=CAB10025.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060052(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060052 NCBI Caenorhabditis elegans H32K16.1 (H32K16.1) mRNA, complete cds. 11/11 None
GENEFINDER00000037439 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:9157841:9161199:1 11/11 None
SNAP00000037437 ENSEMBL cdna:SNAP chromosome:CEL140:I:9157886:9161199:1 11/11 None
H32K16.1 ENSEMBL cdna:known chromosome:CEL140:I:9157841:9161199:1 gene:H32K16.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:9157771-9161130(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl H32K16.1
Entrez Gene 186798 Entrez gene
SwissProt O62286 EMBL-EBI
WormBase CE16186 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6865 amino acid transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5279 amino acid-polyamine transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB10025 Hypothetical protein H32K16.1 [Caenorhabditis elegans] ref|NP_492453.1| H32K16.1 [Caenorhabditis elegans] 0.0
blast CAE67048 Hypothetical protein CBG12455 [Caenorhabditis briggsae] 0.0
blast CAE58132 Hypothetical protein CBG01220 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002422 EMBL-EBI Amino acid/polyamine transporter II 1.0E-126
Pfam IPR002422 EMBL-EBI Amino acid/polyamine transporter II 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492453.1 11 67-86,91-110,156-178,193-215,222-244,259-281,302-324,344-366,387-409,419-438,451-473
H32K16.1 11 67-86,91-110,156-178,193-215,222-244,259-281,302-324,344-366,387-409,419-438,451-473
GENEFINDER00000037439 11 67-86,91-110,156-178,193-215,222-244,259-281,302-324,344-366,387-409,419-438,451-473
SNAP00000037437 11 52-71,76-95,141-163,178-200,207-229,244-266,287-309,329-351,372-394,404-423,436-458
Sequence
Target Sequence 
>C. ELEGANS:180284_AT
gagggtcaagcgatggttctgcccatggaaaattcgttgaaatatccaaaagatatgact
ggagccactggagtcttgtctactgcaatgaatcttgtcacagttctttacgcttttctg
ggatttttcggttacgtcacttttggaccagctgtacaagggagtttaacgttaaatctt
ccaaattctatcttaacagtttcaatcaaaggacttctagtattgaaaatcttttttgga
agtgcaattcaattgtacgtgattgttcaaatgcttcttccaagtcttcgatcaaaaatc
tctgaagatcgcaaaatggttcatcgacttcttccgtatgctcttcgtcttggtcttatg
ctcatttctctatgcattgcccttatcgtaccaaatttgatgcaaatcattccattagtt
ggaataacttcaggacttttgatatctctaattcttccatcatttttggattgcatggta
tttttgccagttttcaaaaaacaaggagacatgttcaaattctaccagaaactaataata
aacgtatttctttttgttcttggatggtttttccttggcgctggactctattcaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGGGTCAAGCGATGGTTCTGCCCA 141 403 832 Antisense
CTGGAGCCACTGGAGTCTTGTCTAC 463 241 890 Antisense
TTGTCACAGTTCTTTACGCTTTTCT 87 703 926 Antisense
TCTGGGATTTTTCGGTTACGTCACT 53 607 948 Antisense
GTTACGTCACTTTTGGACCAGCTGT 566 445 962 Antisense
GCTTCTTCCAAGTCTTCGATCAAAA 493 305 1104 Antisense
AAAATGGTTCATCGACTTCTTCCGT 348 133 1144 Antisense
TTCGTCTTGGTCTTATGCTCATTTC 242 693 1175 Antisense
CTCTATGCATTGCCCTTATCGTACC 705 229 1199 Antisense
TCTTGGATGGTTTTTCCTTGGCGCT 123 617 1389 Antisense
TCCTTGGCGCTGGACTCTATTCAAG 434 591 1403 Antisense
Sequence Source Affymetrix Proprietary Database