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[180283_s_at]

Description F25G6.1 /REP_DB=WormBase Gene ID /WP=CE17703 /TR=O16930 /GB=AAC17742.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 13      4grp_total > 47      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 72
1 2 3 4 range
A 75.5
(3)
A 		11.1
(-2.5)
A 		12.7
(-2.4)
A 		5.2
(-3)
A 		70
B 59.9
(3)
A 		9.1
(-3)
A 		10.9
(-2.8)
A 		37.8
(0.4)
A 		51
C 60.1
(3)
A 		14.1
(-1.9)
A 		3.3
(-3)
A 		11.6
(-2.1)
A 		57
avr 65.2
(3) 		11.4
(-2.7) 		9
(-3) 		18.2
(-2) 		56

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 69
1 2 3 4 5 6 range
A 75.5
(2.9)
A 		76.2
(3)
A 		27.6
(-1.5)
A 		49.5
(0.5)
A 		12.5
(-2.9)
A 		11.6
(-3)
A 		65
B 68.3
(3)
A 		42.4
(0.5)
A 		60.4
(2.2)
A 		18.6
(-1.9)
A 		7.2
(-3)
A 		7.8
(-2.9)
A 		61
C 32.7
(-1.2)
A 		75.3
(3)
A 		16.2
(-2.9)
A 		47.9
(0.3)
A 		29.8
(-1.5)
A 		14.9
(-3)
A 		60
avr 58.8
(2.3) 		64.6
(3) 		34.7
(-0.4) 		38.7
(0.1) 		16.5
(-2.4) 		11.4
(-3) 		53
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 27
1 2 3 4 range
A 27.5
(3)
A 		2.4
(-2.6)
A 		3.3
(-2.4)
A 		0.8
(-3)
A 		27
B 19.1
(3)
A 		2.2
(-3)
A 		2.5
(-2.9)
A 		8.1
(-0.9)
A 		17
C 19.9
(3)
A 		4
(-2.1)
A 		1.2
(-3)
A 		2
(-2.7)
A 		19
avr 22.2
(3) 		2.9
(-2.8) 		2.3
(-3) 		3.6
(-2.6) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 13.9
(-0.3)
A 		27.1
(3)
A 		6
(-2.2)
A 		12.5
(-0.6)
A 		3.8
(-2.8)
A 		2.8
(-3)
A 		24
B 23.8
(3)
A 		14.1
(0.2)
A 		14.7
(0.4)
A 		4.9
(-2.4)
A 		2.9
(-3)
A 		2.9
(-3)
A 		21
C 12.1
(-0.5)
A 		23
(3)
A 		4.1
(-3)
A 		9.6
(-1.3)
A 		9.6
(-1.3)
A 		5
(-2.7)
A 		19
avr 16.6
(1.4) 		21.4
(3) 		8.3
(-1.4) 		9
(-1.2) 		5.4
(-2.4) 		3.6
(-3) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180283_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11456
Sequence Type Exemplar sequence
Representative Public ID F25G6.1 NCBI
Target Description F25G6.1 /REP_DB=WormBase Gene ID /WP=CE17703 /TR=O16930 /GB=AAC17742.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072810(11), NM_074274(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072810 NCBI Caenorhabditis elegans F25G6.1 (F25G6.1) mRNA, complete cds. 11/11 None
NM_074274 NCBI Caenorhabditis elegans H12D21.8 (H12D21.8) mRNA, complete cds. 11/11 A 
SNAP00000026969 ENSEMBL cdna:SNAP chromosome:CEL140:V:14906498:14909089:1 11/11 None
GENEFINDER00000026980 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14905672:14909089:1 11/11 None
F25G6.1 ENSEMBL cdna:known chromosome:CEL140:V:8578730:8582528:1 gene:F25G6.1 11/11 None
H12D21.8 ENSEMBL cdna:known chromosome:CEL140:V:14905672:14909192:1 gene:H12D21.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Public Domain and Genome References
Gene Title

Ensembl F25G6.1
H12D21.8
Entrez Gene 179239 Entrez gene
259665 Entrez gene
SwissProt Q95X84 EMBL-EBI
Q9XTX1 EMBL-EBI
WormBase CE32877 Wormbase
CE19921 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK84524 Hypothetical protein F25G6.1 [Caenorhabditis elegans] ref|NP_505211.3| F25G6.1 [Caenorhabditis elegans] 0.0
blast CAB07426 Hypothetical protein H12D21.8 [Caenorhabditis elegans] ref|NP_506675.1| H12D21.8 [Caenorhabditis elegans] 0.0
blast CAC14422 Hypothetical protein Y71A12B.7 [Caenorhabditis elegans] ref|NP_493432.1| Y71A12B.7 [Caenorhabditis elegans] 0.0
blast T33287 hypothetical protein H20H17.1 - Caenorhabditis elegans 0.0
Sequence
Target Sequence 
>C. ELEGANS:180283_S_AT
cagacggagtgtttttctattcccgccttcatattgatacgacgtataaccttggtgacg
catacgtcactgttattcttggagaaactatgaactttgtgactaccagctccgacaaat
gccgagtactgccattgggatacatggttcacacaaaccgattagctgcaactcacgaaa
agtttgctgattttctttcagaagaatttgccaaagttggcggcgttccgaatggtaaaa
tgattccatgtttgctgatggatggtgaatcaagtttgggagagtatggaaaaacgctcg
acgtgatgaccattcgatgtgactatcacatcttttctttgatgagccataaatatggaa
aacctgtcgccaccaaatcaaaaccttatgtttttggaactatggaaaacgaaaagtgga
agactggacttctaggtgtattcact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAGACGGAGTGTTTTTCTATTCCCG 584 205 821 Antisense
CTATTCCCGCCTTCATATTGATACG 44 225 837 Antisense
GACGCATACGTCACTGTTATTCTTG 690 379 877 Antisense
TATGAACTTTGTGACTACCAGCTCC 674 653 909 Antisense
CTACCAGCTCCGACAAATGCCGAGT 548 225 923 Antisense
TGCCGAGTACTGCCATTGGGATACA 530 585 940 Antisense
AAACCGATTAGCTGCAACTCACGAA 114 127 975 Antisense
AAACGCTCGACGTGATGACCATTCG 292 127 1112 Antisense
TGTGACTATCACATCTTTTCTTTGA 17 557 1138 Antisense
CTGTCGCCACCAAATCAAAACCTTA 495 239 1184 Antisense
GACTGGACTTCTAGGTGTATTCACT 10 377 1242 Antisense
Sequence Source Affymetrix Proprietary Database