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[180254_s_at]

Description F32D8.5B /REP_DB=WormBase Gene ID /WP=CE23699 /TR=Q9U3H0 /GB=CAB54239.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 63      6grp_high1_10_10 > 36      4grp_highest_8_8 > 09      4grp_high_8_8 > 54      6grp_highest_nonorm > 72      6grp_total > 45      4grp_total > 57      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 493
1 2 3 4 range
A 438
(-1.8)
P 		654
(3)
P 		593.5
(1.7)
P 		384.4
(-3)
P 		270
B 548.1
(-0.6)
P 		657.8
(0.7)
P 		844.8
(3)
P 		352.1
(-3)
P 		493
C 470
(-2.2)
P 		634.5
(3)
P 		632.1
(2.9)
P 		445.6
(-3)
P 		189
avr 485.4
(-1.1) 		648.8
(2.2) 		690.1
(3) 		394
(-3) 		296

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 286
1 2 3 4 5 6 range
A 451.8
(3)
P 		451.5
(3)
P 		404.3
(-2.3)
P 		447.3
(2.5)
P 		402.7
(-2.4)
P 		397.7
(-3)
P 		54
B 575.4
(3)
P 		469.1
(-0.2)
P 		472
(-0.1)
P 		386.8
(-2.7)
P 		377.4
(-3)
P 		538.8
(1.9)
P 		198
C 423
(0.4)
P 		482.7
(1.9)
P 		486.9
(2.1)
P 		289.6
(-3)
P 		322.6
(-2.2)
P 		523.8
(3)
P 		234
avr 483.4
(2.8) 		467.8
(2) 		454.4
(1.4) 		374.6
(-2.6) 		367.6
(-3) 		486.8
(3) 		119
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 164
1 2 3 4 range
A 159.5
(3)
P 		142
(2)
P 		155.1
(2.7)
P 		56.3
(-3)
P 		103
B 174.8
(2)
P 		162.5
(1.3)
P 		195.8
(3)
P 		75
(-3)
P 		121
C 155.6
(0.3)
P 		182.4
(1.4)
P 		220.3
(3)
P 		75.1
(-3)
P 		145
avr 163.3
(1.7) 		162.3
(1.6) 		190.4
(3) 		68.8
(-3) 		122

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 143
1 2 3 4 5 6 range
A 83.3
(-3)
P 		160.6
(3)
P 		88.1
(-2.6)
P 		113.5
(-0.7)
P 		121.5
(-0)
P 		96.8
(-2)
P 		77
B 200.2
(3)
P 		156.2
(0.3)
P 		114.6
(-2.3)
P 		102.4
(-3)
P 		153.3
(0.1)
P 		200.9
(3)
P 		99
C 156.8
(2)
P 		147.3
(1.6)
P 		123.9
(0.4)
P 		58.2
(-3)
P 		104.4
(-0.6)
P 		175.4
(3)
P 		117
avr 146.8
(2) 		154.7
(2.7) 		108.9
(-1.4) 		91.4
(-3) 		126.4
(0.2) 		157.7
(3) 		66
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180254_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12725
Sequence Type Exemplar sequence
Representative Public ID F32D8.5B NCBI
Target Description F32D8.5B /REP_DB=WormBase Gene ID /WP=CE23699 /TR=Q9U3H0 /GB=CAB54239.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073376(11), NM_073375(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073376 NCBI Caenorhabditis elegans F32D8.5a (F32D8.5) mRNA, complete cds. 11/11 None
NM_073375 NCBI Caenorhabditis elegans F32D8.5b (F32D8.5) mRNA, complete cds. 11/11 None
SNAP00000041030 ENSEMBL cdna:SNAP chromosome:CEL140:V:10897514:10898385:1 11/11 None
GENEFINDER00000041041 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10897514:10898385:1 11/11 None
F32D8.5a ENSEMBL cdna:known chromosome:CEL140:V:10897454:10898385:1 gene:F32D8.5 11/11 None
F32D8.5b.2 ENSEMBL cdna:known chromosome:CEL140:V:10897478:10898769:1 gene:F32D8.5 11/11 None
F32D8.5b.1 ENSEMBL cdna:known chromosome:CEL140:V:10897478:10899123:1 gene:F32D8.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10897513-10898385(+) 100.0 100.0
Public Domain and Genome References
Ensembl F32D8.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB54239 Hypothetical protein F32D8.5b [Caenorhabditis elegans] ref|NP_505776.1| F32D8.5b [Caenorhabditis elegans] 1.0E-114
blast CAA98457 Hypothetical protein F32D8.5a [Caenorhabditis elegans] ref|NP_505777.1| F32D8.5a [Caenorhabditis elegans] 1.0E-113
blast CAB54239 Hypothetical protein F32D8.5b [Caenorhabditis elegans] ref|NP_505776.1| F32D8.5b [Caenorhabditis elegans] 1.0E-111
blast CAA98457 Hypothetical protein F32D8.5a [Caenorhabditis elegans] ref|NP_505777.1| F32D8.5a [Caenorhabditis elegans] 1.0E-111
Protein Domains
Database ID Description E-Value
Pfam IPR009724 EMBL-EBI Protein of unknown function DUF1301 1.2E-100
Pfam IPR009724 EMBL-EBI Protein of unknown function DUF1301 1.2E-100
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505777.1 2 78-100,110-132
NP_505776.1 2 80-102,112-134
F32D8.5a 2 78-100,110-132
F32D8.5b.2 2 80-102,112-134
F32D8.5b.1 2 80-102,112-134
SNAP00000041030 2 78-100,110-132
GENEFINDER00000041041 2 78-100,110-132
Sequence
Target Sequence 
>C. ELEGANS:180254_S_AT
gcaacgtcgaagttctaccattgtcaaagattgataaagatgacttgggacactccattc
taatccacaagggaacattacctcccggatcacaaaaaactcctcatgatgtagtcgcga
tgggtgtgaaaggagctaaaattctgtcactgtcttcatctcttgccggagtagtcatgg
ttcccgtgctatcctcctatttgtgggaagcagctgctgaaagaccttcgatgatgatgt
ttgcaattgtggccaacaccttcctcgtcctgctatctttcactccgctactacttcatt
ttcttgcaaaacgatttcccatcgacatattttacaataatgacaaaaaggtgttcacaa
ctgttcattacaatttcttaatgcaaaaacaagcattgagattctcagcttctgaagttg
tcgatgcagccgtagctcccgaaatgaagaaagtatggatccctctggcaacagcttttg
tgggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAACGTCGAAGTTCTACCATTGTC 151 321 110 Antisense
GACTTGGGACACTCCATTCTAATCC 85 371 151 Antisense
AAACTCCTCATGATGTAGTCGCGAT 602 127 206 Antisense
AAAATTCTGTCACTGTCTTCATCTC 109 135 247 Antisense
TTGCCGGAGTAGTCATGGTTCCCGT 290 701 272 Antisense
CGTGCTATCCTCCTATTTGTGGGAA 280 245 294 Antisense
TTTGCAATTGTGGCCAACACCTTCC 551 667 349 Antisense
GATTTCCCATCGACATATTTTACAA 86 429 422 Antisense
GAAGTTGTCGATGCAGCCGTAGCTC 497 337 523 Antisense
AGAAAGTATGGATCCCTCTGGCAAC 223 73 557 Antisense
CCCTCTGGCAACAGCTTTTGTGGGC 629 259 570 Antisense
Sequence Source Affymetrix Proprietary Database