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[180249_at]

Description K07E8.6 /REP_DB=WormBase Gene ID /WP=CE11896 /TR=O16685 /GB=AAB66151.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 04      4grp_total > 14      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 90
1 2 3 4 range
A 41.5
(-0.4)
A 		13.8
(-3)
A 		14
(-3)
A 		77.7
(3)
A 		64
B 65
(3)
A 		20.7
(-2)
A 		32.5
(-0.7)
A 		11.9
(-3)
A 		53
C 73.1
(-3)
A 		78.6
(-1.9)
A 		73.6
(-2.9)
A 		102
(3)
A 		29
avr 59.9
(2.1) 		37.7
(-3) 		40
(-2.5) 		63.9
(3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 94
1 2 3 4 5 6 range
A 66.6
(2.2)
A 		74.8
(3)
A 		14.6
(-2.6)
A 		50.5
(0.7)
A 		43.7
(0.1)
A 		10.8
(-3)
A 		64
B 81.9
(1.4)
A 		17
(-3)
A 		105
(3)
A 		70.4
(0.6)
A 		55.7
(-0.4)
A 		22.9
(-2.6)
A 		88
C 80.5
(3)
A 		76.1
(2.5)
A 		55.5
(0.3)
A 		66.5
(1.5)
A 		64.1
(1.2)
A 		24.3
(-3)
A 		56
avr 76.3
(3) 		56
(0.9) 		58.4
(1.1) 		62.5
(1.5) 		54.5
(0.7) 		19.3
(-3) 		57
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 15.1
(3)
A 		3
(-3)
A 		3.7
(-2.7)
A 		11.4
(1.2)
A 		12
B 20.7
(3)
A 		5.1
(-2.1)
A 		7.5
(-1.4)
A 		2.5
(-3)
A 		18
C 24.2
(1.9)
A 		22.6
(0.8)
A 		25.7
(3)
A 		17.2
(-3)
A 		9
avr 20
(3) 		10.2
(-3) 		12.3
(-1.7) 		10.4
(-2.9) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 27
1 2 3 4 5 6 range
A 12.3
(-0.6)
A 		26.6
(3)
A 		3.2
(-2.9)
A 		12.8
(-0.5)
A 		13.2
(-0.4)
A 		2.6
(-3)
A 		24
B 28.5
(3)
A 		5.6
(-3)
A 		25.5
(2.2)
A 		18.6
(0.4)
A 		22.6
(1.5)
A 		8.5
(-2.2)
A 		23
C 29.8
(3)
A 		23.2
(1.2)
A 		14.1
(-1.3)
A 		13.4
(-1.5)
A 		20.7
(0.5)
A 		8.1
(-3)
A 		22
avr 23.5
(3) 		18.5
(1.2) 		14.3
(-0.2) 		14.9
(-0) 		18.8
(1.4) 		6.4
(-3) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180249_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5357
Sequence Type Exemplar sequence
Representative Public ID K07E8.6 NCBI
Target Description K07E8.6 /REP_DB=WormBase Gene ID /WP=CE11896 /TR=O16685 /GB=AAB66151.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061376(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061376 NCBI Caenorhabditis elegans K07E8.6 (K07E8.6) mRNA, complete cds. 11/11 None
SNAP00000018448 ENSEMBL cdna:SNAP chromosome:CEL140:II:638220:638633:-1 11/11 None
GENEFINDER00000018459 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:638092:639438:-1 11/11 None
K07E8.6 ENSEMBL cdna:known chromosome:CEL140:II:638154:638633:-1 gene:K07E8.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:638153-638633(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K07E8.6
Entrez Gene 187114 Entrez gene
SwissProt O16685 EMBL-EBI
WormBase CE11896 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB66151 Hypothetical protein K07E8.6 [Caenorhabditis elegans] ref|NP_493777.1| K07E8.6 [Caenorhabditis elegans] 1.0E-75
blast AAB66151 Hypothetical protein K07E8.6 [Caenorhabditis elegans] ref|NP_493777.1| K07E8.6 [Caenorhabditis elegans] 3.0E-65
blast AAB66151 Hypothetical protein K07E8.6 [Caenorhabditis elegans] ref|NP_493777.1| K07E8.6 [Caenorhabditis elegans] 1.0E-64
blast AAC71172 Hypothetical protein W08F4.8 [Caenorhabditis elegans] ref|NP_493761.1| W08F4.8 [Caenorhabditis elegans] sp|O02108|CDC37_CAEEL Probable Hsp90 co-chaperone Cdc37 (Hsp90 chaperone protein kinase-targeting subunit) 3.0E-25
blast AAC71172 Hypothetical protein W08F4.8 [Caenorhabditis elegans] ref|NP_493761.1| W08F4.8 [Caenorhabditis elegans] sp|O02108|CDC37_CAEEL Probable Hsp90 co-chaperone Cdc37 (Hsp90 chaperone protein kinase-targeting subunit) 6.0E-25
blast AAC71172 Hypothetical protein W08F4.8 [Caenorhabditis elegans] ref|NP_493761.1| W08F4.8 [Caenorhabditis elegans] sp|O02108|CDC37_CAEEL Probable Hsp90 co-chaperone Cdc37 (Hsp90 chaperone protein kinase-targeting subunit) 2.0E-24
blast CAE62901 Hypothetical protein CBG07091 [Caenorhabditis briggsae] 2.0E-21
blast CAE62901 Hypothetical protein CBG07091 [Caenorhabditis briggsae] 7.0E-21
Sequence
Target Sequence 
>C. ELEGANS:180249_AT
gctctactggagcagctctctgagctccaaggtagcactgagccatttctcacggagcat
ccacatatggcagcggagcacacagtaaattggctcacaattgcagcacttagctcagca
atcgacaaaaatgaagaaaaattggcagctttatcagaaaaatgcgttgtttttcaactt
ttcctggcgtccgccacaggtcagaagcaatttttcaaaaaattcgaagtttctgagaat
ctggatgaggttaaagcttttaagagccggatgaggagcagagctcaggcaaaaggagag
aaaccagagaaaattaatttgaatcaaggtactgtcgattctaggcagtttttggagg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTCTACTGGAGCAGCTCTCTGAGC 17 301 40 Antisense
GCTCTCTGAGCTCCAAGGTAGCACT 522 299 54 Antisense
TAGCACTGAGCCATTTCTCACGGAG 376 651 72 Antisense
GGAGCATCCACATATGGCAGCGGAG 406 521 93 Antisense
GGCAGCGGAGCACACAGTAAATTGG 222 519 108 Antisense
ATTGGCTCACAATTGCAGCACTTAG 163 3 128 Antisense
GCAGCACTTAGCTCAGCAATCGACA 238 313 142 Antisense
CGTCCGCCACAGGTCAGAAGCAATT 422 245 227 Antisense
GCCGGATGAGGAGCAGAGCTCAGGC 351 283 305 Antisense
ATCAAGGTACTGTCGATTCTAGGCA 87 27 362 Antisense
GTCGATTCTAGGCAGTTTTTGGAGG 666 471 373 Antisense
Sequence Source Affymetrix Proprietary Database