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[180247_s_at]

Description R04F11.3 /REP_DB=WormBase Gene ID /WP=CE06234 /TR=Q21730 /GB=CAA98958.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 77      4grp_highest_8_8 > 37      4grp_high_8_8 > 03      6grp_highest_nonorm > 92      6grp_total > 77      4grp_total > 06      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 342
1 2 3 4 range
A 313.3
(3)
P 		137.6
(-3)
P 		233.7
(0.3)
P 		238.4
(0.4)
P 		176
B 220.7
(-2.1)
P 		228.6
(-1.9)
P 		173.1
(-3)
P 		479.3
(3)
P 		306
C 307.8
(2.7)
P 		138.2
(-3)
P 		262.1
(1.2)
P 		317.3
(3)
P 		179
avr 280.6
(0.8) 		168.1
(-3) 		223
(-1.1) 		345
(3) 		177

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 465
1 2 3 4 5 6 range
A 193.4
(-3)
P 		284
(-0.6)
P 		200
(-2.8)
P 		327.8
(0.5)
P 		288
(-0.5)
P 		424.2
(3)
P 		231
B 241.4
(-1.9)
P 		187.7
(-2.8)
P 		178.1
(-3)
P 		210.8
(-2.4)
P 		346.7
(0)
P 		515.3
(3)
P 		337
C 267.3
(-2.8)
P 		281.1
(-2.6)
P 		252.7
(-3)
P 		259.1
(-2.9)
P 		256.6
(-2.9)
P 		643.2
(3)
P 		391
avr 234
(-2.6) 		250.9
(-2.2) 		210.3
(-3) 		265.9
(-1.9) 		297.1
(-1.4) 		527.6
(3) 		317
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 84
1 2 3 4 range
A 114.1
(3)
P 		29.9
(-3)
P 		61.1
(-0.8)
P 		34.9
(-2.6)
P 		84
B 70.4
(-0.1)
P 		56.5
(-1.4)
P 		40.1
(-3)
P 		102.1
(3)
P 		62
C 101.9
(3)
P 		39.7
(-3)
P 		91.3
(2)
P 		53.5
(-1.7)
P 		62
avr 95.5
(3) 		42
(-3) 		64.2
(-0.5) 		63.5
(-0.6) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 180
1 2 3 4 5 6 range
A 35.7
(-3)
P 		101
(2.8)
P 		43.5
(-2.3)
P 		83.1
(1.2)
P 		86.9
(1.5)
P 		103.3
(3)
P 		68
B 84
(-1.4)
P 		62.5
(-2.2)
P 		43.2
(-3)
P 		55.8
(-2.5)
P 		140.9
(0.9)
P 		192.2
(3)
P 		149
C 99.1
(-1.3)
P 		85.8
(-1.8)
P 		64.3
(-2.6)
P 		52.1
(-3)
P 		83
(-1.9)
P 		215.3
(3)
P 		163
avr 72.9
(-1.9) 		83.1
(-1.4) 		50.3
(-3) 		63.7
(-2.3) 		103.6
(-0.3) 		170.3
(3) 		120
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180247_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13089
Sequence Type Exemplar sequence
Representative Public ID R04F11.3 NCBI
Target Description R04F11.3 /REP_DB=WormBase Gene ID /WP=CE06234 /TR=Q21730 /GB=CAA98958.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073687(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073687 NCBI Caenorhabditis elegans R04F11.3 (R04F11.3) mRNA, complete cds. 11/11 None
SNAP00000007838 ENSEMBL cdna:SNAP chromosome:CEL140:V:12317713:12319768:-1 11/11 None
GENEFINDER00000007846 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12317713:12319768:-1 11/11 None
R04F11.3.2 ENSEMBL cdna:known chromosome:CEL140:V:12317596:12319768:-1 gene:R04F11.3 11/11 None
R04F11.3.1 ENSEMBL cdna:known chromosome:CEL140:V:12317596:12319770:-1 gene:R04F11.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12317712-12320538(-) 98.13 100.0
Public Domain and Genome References
Gene Title
Ensembl R04F11.3
Entrez Gene 179688 Entrez gene
SwissProt Q21730 EMBL-EBI
WormBase CE29598 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA98958 Hypothetical protein R04F11.3 [Caenorhabditis elegans] ref|NP_506088.1| R04F11.3 [Caenorhabditis elegans] 1.0E-173
blast CAE75455 Hypothetical protein CBG23450 [Caenorhabditis briggsae] 1.0E-103
Sequence
Target Sequence 
>C. ELEGANS:180247_S_AT
gaactctgagtatgttttccaaaaaaactgaaaagaaaactgaaaagactgagaaatctc
aatcccctgttcctcaagatcaagaaaagaaaaatcgtaaacgtcccatgatagaagtcg
atgagtctgaagaacttattaaggccactaaaccaaaagaaaagacaccagagaaaaaac
cggaaaaattgattatttctcaagacagtgatgaatttccatcatcaagagagcaaagtg
tggagaaggaaaagtcaccaccagcaaagcgaaaaagggttaatgatgagaacaagaaag
cgaagaagagacttattgtaccggattctgacgatgaagagcaagttccaatggaggtcg
aagaaaagaaaacggaaacaaaaacaaactcatctttgccagaagatagacggagaacca
cacgaatcgaaaaggttgtcgagacatatgttgatgaagatggttatttggtatccaagg
cagttcaaaaggctgtagaatgttctccacaaaaagcatcacccgaaaagcccaaaccga
aagtgataactgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACTCTGAGTATGTTTTCCAAAAA 694 347 728 Antisense
ACTGAGAAATCTCAATCCCCTGTTC 591 97 775 Antisense
ATCCCCTGTTCCTCAAGATCAAGAA 656 37 789 Antisense
GTAAACGTCCCATGATAGAAGTCGA 693 451 824 Antisense
GAATTTCCATCATCAAGAGAGCAAA 595 325 940 Antisense
AAAAGTCACCACCAGCAAAGCGAAA 47 133 977 Antisense
GAGACTTATTGTACCGGATTCTGAC 627 393 1035 Antisense
AAAACAAACTCATCTTTGCCAGAAG 693 127 1108 Antisense
TAGACGGAGAACCACACGAATCGAA 450 651 1134 Antisense
TGTTCTCCACAAAAAGCATCACCCG 205 563 1228 Antisense
AGCCCAAACCGAAAGTGATAACTGC 460 81 1256 Antisense
Sequence Source Affymetrix Proprietary Database