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[180245_at]

Description F22E5.7 /REP_DB=WormBase Gene ID /WP=CE27993 /TR=O16707 /GB=AAK68345.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 11      4grp_total > 36      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 14
(-3)
A 		62.5
(3)
P 		18.1
(-2.5)
A 		48.7
(1.3)
A 		49
B 40.8
(3)
A 		33.5
(1.4)
A 		12.7
(-3)
A 		19.1
(-1.6)
A 		28
C 40.4
(0.4)
A 		58.8
(3)
A 		17
(-3)
A 		28.3
(-1.4)
A 		42
avr 31.7
(-0.3) 		51.6
(3) 		15.9
(-3) 		32
(-0.3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 28.4
(0.9)
A 		23.6
(-0)
A 		8.6
(-3)
A 		38.7
(3)
A 		29.6
(1.2)
A 		34.6
(2.2)
A 		30
B 27.3
(-0.3)
A 		53.2
(3)
P 		44.6
(1.9)
A 		13.1
(-2)
A 		25.3
(-0.5)
A 		5.5
(-3)
A 		48
C 20.1
(-1.6)
A 		49.1
(3)
A 		48.7
(2.9)
A 		13.2
(-2.7)
A 		11.6
(-3)
A 		25.2
(-0.8)
A 		38
avr 25.3
(-1.9) 		42
(3) 		34
(0.6) 		21.7
(-3) 		22.2
(-2.9) 		21.8
(-3) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 5.1
(-2.7)
A 		13.6
(3)
P 		4.7
(-3)
A 		7.1
(-1.4)
A 		9
B 13
(3)
A 		8.3
(0.2)
A 		2.9
(-3)
A 		4.1
(-2.3)
A 		10
C 13.4
(1.3)
A 		16.9
(3)
A 		5.9
(-2.5)
A 		4.8
(-3)
A 		12
avr 10.5
(1.3) 		12.9
(3) 		4.5
(-3) 		5.3
(-2.4) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 5.2
(-0.5)
A 		8.4
(1.9)
A 		1.9
(-3)
A 		9.8
(3)
A 		8.9
(2.3)
A 		8.4
(1.9)
A 		8
B 9.5
(-0.1)
A 		17.7
(3)
P 		10.8
(0.4)
A 		3.5
(-2.4)
A 		10.3
(0.2)
A 		2
(-3)
A 		16
C 7.4
(-0.7)
A 		15
(3)
A 		12.4
(1.7)
A 		2.7
(-3)
A 		3.7
(-2.5)
A 		8.4
(-0.2)
A 		12
avr 7.4
(-1.5) 		13.7
(3) 		8.4
(-0.8) 		5.3
(-3) 		7.6
(-1.4) 		6.3
(-2.3) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180245_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4118
Sequence Type Exemplar sequence
Representative Public ID F22E5.7 NCBI
Target Description F22E5.7 /REP_DB=WormBase Gene ID /WP=CE27993 /TR=O16707 /GB=AAK68345.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061915(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061915 NCBI Caenorhabditis elegans F22E5.7 (F22E5.7) mRNA, complete cds. 9/11 None
F22E5.7 ENSEMBL cdna:known chromosome:CEL140:II:2642001:2642885:1 gene:F22E5.7 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000023605 1/11 Cross Hyb Matching Probes None
GENEFINDER00000023631 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2641525-2642884(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F22E5.7
Entrez Gene 184836 Entrez gene
SwissProt O16707 EMBL-EBI
WormBase CE27993 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_494316 F22E5.7 [Caenorhabditis elegans] gb|AAK68345.1| Hypothetical protein F22E5.7 [Caenorhabditis elegans] 1.0E-63
blast T32066 hypothetical protein F22E5.7 - Caenorhabditis elegans 1.0E-63
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494316.1 1 10-32
F22E5.7 1 10-32
Sequence
Target Sequence 
>C. ELEGANS:180245_AT
aaagtttttcagccattgctcattattggatatgtgcatgataccgatagactgcatagt
atgtcccttccaaaaaatttcaatgtgcgtgccttcattgatacgcattccaatcagttc
gagatttatttcagtgagcacatttatcctacttttatcattccattttcagttttttca
ataaaaatgttatcatcaattagcagcaaacttctgctcctacttcttctaactttttta
ttgactttctcaaactccacaaagttcacagcacttgtgaatttgagatgtcggagccat
gcggtttggtatagccatgcctcgatgtgggagaaggattctctgaccccagacgacctt
ctagcatacgaaggatccacgcggaacgaaaaggaaactcgaatatacaatctcacgagc
gaggacatttgggatgagttccaaagtaatgccttcgaaatacgagtccacgtctcgcat
aactgcagcaaaaatggggttctacaaatccggaaattgaaacgaattcccaagtttttg
ttgctaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAGTTTTTCAGCCATTGCTCATTA 164 123 37 Antisense
GAGCACATTTATCCTACTTTTATCA 560 389 172 Antisense
AGCAGCAAACTTCTGCTCCTACTTC 384 75 238 Antisense
TTATTGACTTTCTCAAACTCCACAA 106 677 274 Antisense
AACTCCACAAAGTTCACAGCACTTG 503 139 289 Antisense
AGATGTCGGAGCCATGCGGTTTGGT 502 67 322 Antisense
CGGTTTGGTATAGCCATGCCTCGAT 391 243 338 Antisense
GGGAGAAGGATTCTCTGACCCCAGA 441 495 365 Antisense
AGCATACGAAGGATCCACGCGGAAC 73 77 399 Antisense
TCCACGTCTCGCATAACTGCAGCAA 222 589 503 Antisense
GAATTCCCAAGTTTTTGTTGCTAGA 6 325 560 Antisense
Sequence Source Affymetrix Proprietary Database