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[180239_at]

Description C32B5.8 /REP_DB=WormBase Gene ID /WP=CE08516 /TR=P91108 /GB=AAB37957.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 33      4grp_total > 15      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 63
1 2 3 4 range
A 33.9
(2)
A 		5.1
(-3)
A 		16.1
(-1.1)
A 		39.8
(3)
A 		35
B 33.4
(3)
A 		26.7
(-1.8)
A 		28.4
(-0.6)
A 		25.1
(-3)
A 		8
C 63.5
(2.4)
A 		19.3
(-3)
A 		19.4
(-3)
A 		68.5
(3)
A 		49
avr 43.6
(2.8) 		17
(-3) 		21.3
(-2.1) 		44.5
(3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 64
1 2 3 4 5 6 range
A 53
(3)
A 		21.5
(-0.7)
A 		50
(2.6)
A 		43.6
(1.9)
A 		3.4
(-2.9)
A 		2.4
(-3)
A 		51
B 40.7
(-0.1)
A 		35.1
(-0.8)
A 		66.2
(3)
A 		31.5
(-1.3)
A 		32.9
(-1.1)
A 		17.3
(-3)
A 		49
C 54.3
(3)
A 		17.5
(-1.5)
A 		37.1
(0.9)
A 		5.5
(-3)
A 		35.3
(0.7)
A 		20.6
(-1.1)
A 		49
avr 49.3
(2.7) 		24.7
(-1.2) 		51.1
(3) 		26.9
(-0.9) 		23.9
(-1.3) 		13.4
(-3) 		38
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 12.3
(3)
A 		1.1
(-3)
A 		4.2
(-1.3)
A 		5.8
(-0.5)
A 		11
B 10.7
(3)
A 		6.6
(-1.6)
A 		6.6
(-1.6)
A 		5.4
(-3)
A 		5
C 21
(3)
A 		5.6
(-3)
A 		6.8
(-2.5)
A 		11.5
(-0.7)
A 		15
avr 14.7
(3) 		4.4
(-3) 		5.9
(-2.1) 		7.6
(-1.1) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 9.8
(2.3)
A 		7.6
(1)
A 		10.9
(2.9)
A 		11.1
(3)
A 		1
(-2.8)
A 		0.6
(-3)
A 		11
B 14.2
(1.8)
A 		11.7
(0.3)
A 		16.1
(3)
A 		8.3
(-1.8)
A 		13.4
(1.3)
A 		6.4
(-3)
A 		10
C 20.1
(3)
A 		5.3
(-1.7)
A 		9.4
(-0.4)
A 		1.1
(-3)
A 		11.4
(0.3)
A 		6.9
(-1.2)
A 		19
avr 14.7
(3) 		8.2
(-0.9) 		12.1
(1.5) 		6.8
(-1.7) 		8.6
(-0.6) 		4.6
(-3) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180239_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4555
Sequence Type Exemplar sequence
Representative Public ID C32B5.8 NCBI
Target Description C32B5.8 /REP_DB=WormBase Gene ID /WP=CE08516 /TR=P91108 /GB=AAB37957.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061463(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061463 NCBI Caenorhabditis elegans C32B5.8 (C32B5.8) mRNA, complete cds. 11/11 None
SNAP00000042173 ENSEMBL cdna:SNAP chromosome:CEL140:II:956680:957967:1 10/11 None
GENEFINDER00000042191 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:954495:957967:1 11/11 None
C32B5.8 ENSEMBL cdna:known chromosome:CEL140:II:956680:957967:1 gene:C32B5.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:956678-957966(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C32B5.8
Entrez Gene 183112 Entrez gene
SwissProt P91108 EMBL-EBI
WormBase CE08516 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_493863 C32B5.9 [Caenorhabditis elegans] gb|AAB37964.1| Hypothetical protein C32B5.9 [Caenorhabditis elegans] 1.0E-166
blast NP_493864 C32B5.8 [Caenorhabditis elegans] gb|AAB37957.1| Hypothetical protein C32B5.8 [Caenorhabditis elegans] 1.0E-154
blast NP_493864 C32B5.8 [Caenorhabditis elegans] gb|AAB37957.1| Hypothetical protein C32B5.8 [Caenorhabditis elegans] 1.0E-153
blast NP_493864 C32B5.8 [Caenorhabditis elegans] gb|AAB37957.1| Hypothetical protein C32B5.8 [Caenorhabditis elegans] 1.0E-123
blast AAC68939 Hypothetical protein C44C8.1 [Caenorhabditis elegans] ref|NP_500010.1| C44C8.1 [Caenorhabditis elegans] 6.0E-99
blast AAC02595 Hypothetical protein F58H7.8 [Caenorhabditis elegans] ref|NP_500011.1| F58H7.8 [Caenorhabditis elegans] 1.0E-85
blast AAC68942 Hypothetical protein C44C8.4 [Caenorhabditis elegans] ref|NP_500007.1| C44C8.4 [Caenorhabditis elegans] 1.0E-85
blast AAC02595 Hypothetical protein F58H7.8 [Caenorhabditis elegans] ref|NP_500011.1| F58H7.8 [Caenorhabditis elegans] 2.0E-75
blast AAC68942 Hypothetical protein C44C8.4 [Caenorhabditis elegans] ref|NP_500007.1| C44C8.4 [Caenorhabditis elegans] 2.0E-75
Sequence
Target Sequence 
>C. ELEGANS:180239_AT
agttgatatgaatctcgtcgtggaagaagatccggaggagattctcgtagacttgagagg
aaatcaagagagaactattgccaatgttgtcacagagctggctaaccagagaaggattga
gaatatcgagaaaatgatacaaagaagaagaaactattttcttgtgctcacagttggaag
ggtccgtgaagtgatgatctatgagaggaagctccacgaggcagtaaagtacttggttga
aaagctattaggtgggagaggtattatcaaagttgacacactttccattcgatctcgagg
gatactgcggataccgtcaagattgaagcgatcaagtttggattttgtgaagcactggat
gttacatggtaaagaaatcggatcttattatacattcgtgattaccgagcagaatataaa
tgaggctatgagtaggattagagaagagtatgaagggattgcttggaagactggggagat
cactataaatatgaaagccgattctcaacttgtaatcaccaagattcaacaaggcgatgc
a

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTTGATATGAATCTCGTCGTGGAA 580 63 264 Antisense
GAAGATCCGGAGGAGATTCTCGTAG 404 337 289 Antisense
GAACTATTGCCAATGTTGTCACAGA 523 349 335 Antisense
GAAACTATTTTCTTGTGCTCACAGT 456 357 413 Antisense
TTGTGCTCACAGTTGGAAGGGTCCG 35 707 425 Antisense
GGAAGGGTCCGTGAAGTGATGATCT 610 529 439 Antisense
AAGCTCCACGAGGCAGTAAAGTACT 288 149 472 Antisense
CACACTTTCCATTCGATCTCGAGGG 85 193 540 Antisense
TATACATTCGTGATTACCGAGCAGA 93 659 652 Antisense
AGCCGATTCTCAACTTGTAATCACC 405 83 759 Antisense
CACCAAGATTCAACAAGGCGATGCA 23 203 780 Antisense
Sequence Source Affymetrix Proprietary Database