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[180225_at]

Description K03B4.1 /REP_DB=WormBase Gene ID /WP=CE07321 /TR=Q21174 /GB=AAA97998.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 87      6grp_high1_10_10 > 87      4grp_highest_8_8 > 39      4grp_high_8_8 > 12      6grp_highest_nonorm > 85      6grp_total > 86      4grp_total > 12      6grp_highest_sub > Da      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 439
1 2 3 4 range
A 718.5
(0.6)
P 		693.9
(0.2)
P 		489
(-3)
P 		871
(3)
P 		382
B 484.6
(-2.1)
P 		432.5
(-3)
P 		446.7
(-2.8)
P 		794.4
(3)
P 		362
C 629.3
(-1.1)
P 		530.9
(-3)
P 		620.1
(-1.3)
P 		846.7
(3)
P 		316
avr 610.8
(-1.3) 		552.4
(-2.4) 		518.6
(-3) 		837.4
(3) 		319

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 455
1 2 3 4 5 6 range
A 692.2
(-0.7)
P 		563.9
(-3)
P 		687.3
(-0.8)
P 		657.1
(-1.3)
P 		719.7
(-0.2)
P 		900.6
(3)
P 		337
B 665.7
(-0.7)
P 		494.7
(-3)
P 		497.8
(-3)
P 		796.4
(1)
P 		781.5
(0.8)
P 		949.5
(3)
P 		455
C 617.7
(-2.9)
P 		628.2
(-2.7)
P 		610.9
(-3)
P 		679.2
(-1.7)
P 		660.8
(-2)
P 		920.8
(3)
P 		310
avr 658.5
(-1.4) 		562.3
(-3) 		598.7
(-2.4) 		710.9
(-0.5) 		720.7
(-0.4) 		923.6
(3) 		361
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 158
1 2 3 4 range
A 261.6
(3)
P 		150.6
(-2)
P 		127.8
(-3)
P 		127.5
(-3)
P 		134
B 154.5
(1.7)
P 		106.8
(-2.7)
P 		103.5
(-3)
P 		169.3
(3)
P 		66
C 208.3
(2.4)
P 		152.6
(-2.2)
P 		216.1
(3)
P 		142.8
(-3)
P 		73
avr 208.1
(3) 		136.7
(-3) 		149.1
(-2) 		146.5
(-2.2) 		71

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 233
1 2 3 4 5 6 range
A 127.6
(-3)
P 		200.5
(1.8)
P 		149.7
(-1.6)
P 		166.7
(-0.4)
P 		217.2
(2.9)
P 		219.3
(3)
P 		92
B 231.6
(-0.2)
P 		164.7
(-1.9)
P 		120.9
(-3)
P 		210.9
(-0.7)
P 		317.5
(2.1)
P 		354.1
(3)
P 		233
C 229
(0.2)
P 		191.7
(-1.1)
P 		155.5
(-2.3)
P 		136.6
(-3)
P 		213.8
(-0.3)
P 		308.2
(3)
P 		172
avr 196.1
(-0.9) 		185.6
(-1.3) 		142
(-3) 		171.4
(-1.8) 		249.5
(1.2) 		293.9
(3) 		152
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180225_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14804
Sequence Type Exemplar sequence
Representative Public ID K03B4.1 NCBI
Target Description K03B4.1 /REP_DB=WormBase Gene ID /WP=CE07321 /TR=Q21174 /GB=AAA97998.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071862(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071862 NCBI Caenorhabditis elegans K03B4.1 (K03B4.1) mRNA, complete cds. 11/11 None
SNAP00000000714 ENSEMBL cdna:SNAP chromosome:CEL140:V:4689181:4691379:1 11/11 None
K03B4.1 ENSEMBL cdna:known chromosome:CEL140:V:4689144:4691412:1 gene:K03B4.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000000716 2/11 Cross Hyb Matching Probes None
GENEFINDER00000000718 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:4689908-4691379(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K03B4.1
Entrez Gene 178859 Entrez gene
SwissProt Q21174 EMBL-EBI
WormBase CE28579 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA97998 Hypothetical protein K03B4.1 [Caenorhabditis elegans] ref|NP_504263.1| K03B4.1 [Caenorhabditis elegans] 0.0
blast CAE64173 Hypothetical protein CBG08796 [Caenorhabditis briggsae] 0.0
blast CAE64173 Hypothetical protein CBG08796 [Caenorhabditis briggsae] 1.0E-176
Sequence
Target Sequence 
>C. ELEGANS:180225_AT
atcaatccacgtgtatcccgtttacagatgactcgagccattttccgctgacctatttgg
ttggaaactcacttcctgctataacttctccattcctaaagacgtcattcggcgagaaaa
atgaatggccaattagtgttcagtgcacaggtgcaagctatacggaaaagaaaaaccaag
ttgatcttggcaacgcgcgacagcggatgaagcattgtgcgttgtggatgagcaaatcag
ccgaagagcttgaaggaatcataaacgacgcaaatgttcgagtcggagcatatttggacg
aggggcttgatctagaagtcaaagttcgagaagttcgtggtagcgagctgaaaaactatg
aatcgcaggcgttcgttgtggagaatcgaggactttcattgtcgaggatctcaaggattg
gggattatgtatcgaaacggctcaatattcaatactcgggtagcccggcaactgaaaaat
ctgaaaaatctgatggattcgttcaaatggtatacgtcgagactgatattgatcgaattc
ttgctcgactcgttgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCAATCCACGTGTATCCCGTTTAC 528 25 173 Antisense
TCCCGTTTACAGATGACTCGAGCCA 216 597 188 Antisense
CCATTTTCCGCTGACCTATTTGGTT 428 269 210 Antisense
GAAACTCACTTCCTGCTATAACTTC 612 355 236 Antisense
TAACTTCTCCATTCCTAAAGACGTC 156 631 254 Antisense
ATCTTGGCAACGCGCGACAGCGGAT 241 29 356 Antisense
ATTTGGACGAGGGGCTTGATCTAGA 44 3 464 Antisense
AATCGCAGGCGTTCGTTGTGGAGAA 316 169 533 Antisense
GAGGACTTTCATTGTCGAGGATCTC 310 405 560 Antisense
AATACTCGGGTAGCCCGGCAACTGA 214 159 623 Antisense
TCGAATTCTTGCTCGACTCGTTGAC 134 601 705 Antisense
Sequence Source Affymetrix Proprietary Database