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[180223_s_at]

Description T23F4.1 /REP_DB=WormBase Gene ID /WP=CE14084 /TR=O17262 /GB=AAB71033.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 82      4grp_high_8_8 > 11      6grp_total > 73      4grp_total > 13      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 322
1 2 3 4 range
A 173.8
(-0.1)
P 		57.4
(-2.2)
A 		16.3
(-3)
A 		337.8
(3)
P 		322
B 190.8
(0.6)
P 		34.7
(-2.9)
A 		31.5
(-3)
A 		298.3
(3)
P 		267
C 165.9
(-0.3)
P 		75.6
(-2)
P 		26.4
(-3)
P 		335.8
(3)
P 		309
avr 176.8
(0) 		55.9
(-2.4) 		24.7
(-3) 		324
(3) 		299

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 375
1 2 3 4 5 6 range
A 57
(-3)
A 		100.7
(-2.2)
P 		305.5
(1.6)
P 		383.3
(3)
P 		355.6
(2.5)
P 		146.2
(-1.4)
P 		326
B 81
(-2.9)
P 		76
(-3)
M 		334.1
(1.4)
P 		431.8
(3)
P 		386.4
(2.2)
P 		195.1
(-1)
P 		356
C 121.4
(-2.2)
P 		80.4
(-3)
P 		347.4
(2.4)
P 		299
(1.4)
P 		376.8
(3)
P 		202.1
(-0.5)
P 		296
avr 86.5
(-3) 		85.7
(-3) 		329
(2.1) 		371.4
(3) 		372.9
(3) 		181.1
(-1) 		287
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 59
1 2 3 4 range
A 63.3
(3)
P 		12.5
(-2.2)
A 		4.3
(-3)
A 		49.4
(1.6)
P 		59
B 60.8
(2.7)
P 		8.6
(-2.9)
A 		7.3
(-3)
A 		63.6
(3)
P 		56
C 54.9
(2.8)
P 		21.7
(-1.4)
P 		9.2
(-3)
P 		56.6
(3)
P 		47
avr 59.7
(3) 		14.3
(-2.2) 		6.9
(-3) 		56.5
(2.6) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 147
1 2 3 4 5 6 range
A 10.5
(-3)
A 		35.8
(-1.4)
P 		66.5
(0.5)
P 		97.2
(2.4)
P 		107.3
(3)
P 		35.6
(-1.4)
P 		97
B 28.2
(-2.9)
P 		25.3
(-3)
M 		81.1
(-0.5)
P 		114.4
(1.1)
P 		157
(3)
P 		72.7
(-0.8)
P 		132
C 45
(-1.7)
P 		24.5
(-3)
P 		88.4
(0.9)
P 		60.1
(-0.8)
P 		121.9
(3)
P 		67.7
(-0.3)
P 		97
avr 27.9
(-3) 		28.5
(-3) 		78.7
(0) 		90.6
(0.7) 		128.7
(3) 		58.7
(-1.2) 		101
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180223_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4648
Sequence Type Exemplar sequence
Representative Public ID T23F4.1 NCBI
Target Description T23F4.1 /REP_DB=WormBase Gene ID /WP=CE14084 /TR=O17262 /GB=AAB71033.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061523(11), NM_061524(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061523 NCBI Caenorhabditis elegans T23F4.1 (T23F4.1) mRNA, complete cds. 11/11 A 
NM_061524 NCBI Caenorhabditis elegans T23F4.3 (T23F4.3) mRNA, complete cds. 11/11 A 
SNAP00000024152 ENSEMBL cdna:SNAP chromosome:CEL140:II:1169895:1180906:-1 11/11 None
GENEFINDER00000024153 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:1166505:1169172:1 11/11 None
GENEFINDER00000024156 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:1169846:1174611:-1 11/11 None
T23F4.1 ENSEMBL cdna:known chromosome:CEL140:II:1166496:1169172:1 gene:T23F4.1 11/11 A 
T23F4.3 ENSEMBL cdna:known chromosome:CEL140:II:1169846:1172090:-1 gene:T23F4.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:1166494-1169171(+) 100.0 100.0
Public Domain and Genome References
Gene Title

Ensembl T23F4.1
T23F4.3
Entrez Gene 188807 Entrez gene
188808 Entrez gene
SwissProt O17262 EMBL-EBI
O17263 EMBL-EBI
WormBase CE14084 Wormbase
CE14088 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
16787 hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB71031 Nematode astacin protease protein 27 [Caenorhabditis elegans] ref|NP_493926.2| T23F4.4 [Caenorhabditis elegans] sp|O17264|NAS27_CAEEL Zinc metalloproteinase nas-27 precursor (Nematode astacin 27) 0.0
blast CAE62910 Hypothetical protein CBG07102 [Caenorhabditis briggsae] 0.0
blast AAB71033 Hypothetical protein T23F4.1 [Caenorhabditis elegans] ref|NP_493924.1| T23F4.1 [Caenorhabditis elegans] 1.0E-154
blast AAB71032 Hypothetical protein T23F4.3 [Caenorhabditis elegans] ref|NP_493925.1| T23F4.3 [Caenorhabditis elegans] 1.0E-152
blast AAB71033 Hypothetical protein T23F4.1 [Caenorhabditis elegans] ref|NP_493924.1| T23F4.1 [Caenorhabditis elegans] 1.0E-147
blast AAB71032 Hypothetical protein T23F4.3 [Caenorhabditis elegans] ref|NP_493925.1| T23F4.3 [Caenorhabditis elegans] 1.0E-125
blast CAE62911 Hypothetical protein CBG07103 [Caenorhabditis briggsae] 1.0E-121
Protein Domains
Database ID Description E-Value
Pfam IPR002925 EMBL-EBI Dienelactone hydrolase 4.0E-94
Pfam IPR002925 EMBL-EBI Dienelactone hydrolase 6.9E-92
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 9.5E-90
Pfam IPR002925 EMBL-EBI Dienelactone hydrolase 5.7E-78
Pfam IPR002925 EMBL-EBI Dienelactone hydrolase 5.3E-15
Pfam IPR002925 EMBL-EBI Dienelactone hydrolase 2.6E-72
Pfam IPR005515 EMBL-EBI Vitelline membrane outer layer protein I (VOMI) 7.2E-8
Pfam IPR005515 EMBL-EBI Vitelline membrane outer layer protein I (VOMI) 3.5E-9
Pfam IPR002925 EMBL-EBI Dienelactone hydrolase 4.8E-86
Sequence
Target Sequence 
>C. ELEGANS:180223_S_AT
catcttccatgctttcactggacgtaccgaatttgataatcagaaggctagggatttggc
taaggtagctcggctacgtagcttttgccgcagatacctatggaaaaggaaaagccgctc
cagatgtgaatggaaattttgccattatgggagaaccacaaccctccgcagctggattct
gagtgcctggaactatgtaagaggtctaaaatatgtccaaactaacaatatcgggtcaat
tgggttctgttttggcgggctttgtactttggatttggcccgtttcaatgttggactaaa
agctgctgtgtcattccatggaacacttacggattacccgggaaatggaaccacaatcga
cgcttcaatccaagctcatcacggagatgcagaccctcacactacaaatcaaaacgccga
tgatttcattgaagaaatgaggagaagaaacggtgattggatgttcagtagatacgccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CATCTTCCATGCTTTCACTGGACGT 378 213 189 Antisense
CTGGACGTACCGAATTTGATAATCA 566 241 206 Antisense
ATCAGAAGGCTAGGGATTTGGCTAA 95 27 227 Antisense
ATATCGGGTCAATTGGGTTCTGTTT 154 21 416 Antisense
CAATTGGGTTCTGTTTTGGCGGGCT 365 179 425 Antisense
TGTTTTGGCGGGCTTTGTACTTTGG 119 547 436 Antisense
TGTACTTTGGATTTGGCCCGTTTCA 379 559 451 Antisense
GATTTGGCCCGTTTCAATGTTGGAC 523 427 460 Antisense
AATGTTGGACTAAAAGCTGCTGTGT 393 163 475 Antisense
CCATGGAACACTTACGGATTACCCG 236 265 504 Antisense
GATTGGATGTTCAGTAGATACGCCC 391 435 643 Antisense
Sequence Source Affymetrix Proprietary Database