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[180221_at]

Description F54E2.5 /REP_DB=WormBase Gene ID /WP=CE17109 /TR=Q9TXK0 /GB=AAC78201.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 33      4grp_high_8_8 > 35      6grp_total > 92      4grp_total > 29      6grp_high1_sub > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 45
1 2 3 4 range
A 60.3
(0.3)
P 		47.6
(-3)
A 		47.5
(-3)
A 		70.6
(3)
A 		23
B 35
(-0.9)
P 		52.1
(3)
P 		26
(-3)
A 		48
(2.1)
A 		26
C 57
(3)
P 		47.7
(0.8)
P 		32
(-2.9)
P 		31.7
(-3)
P 		25
avr 50.8
(3) 		49.1
(2.3) 		35.2
(-3) 		50.1
(2.7) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 64
1 2 3 4 5 6 range
A 43.9
(0.7)
A 		15.9
(-1.9)
A 		25.9
(-1)
P 		62.1
(2.4)
P 		68.6
(3)
P 		4.5
(-3)
A 		64
B 59.4
(2.3)
P 		62.4
(3)
P 		61
(2.7)
P 		38.5
(-2.7)
P 		40.8
(-2.1)
P 		37.1
(-3)
P 		25
C 37.6
(-3)
P 		43.7
(3)
A 		38.2
(-2.4)
A 		42.3
(1.6)
A 		38
(-2.6)
A 		41.8
(1.1)
P 		6
avr 47
(2.4) 		40.7
(0.6) 		41.7
(0.9) 		47.6
(2.6) 		49.1
(3) 		27.8
(-3) 		21
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 22
(3)
P 		10.3
(-3)
A 		12.4
(-1.9)
A 		10.3
(-3)
A 		12
B 11.2
(1.5)
P 		12.9
(3)
P 		6
(-3)
A 		10.2
(0.7)
A 		7
C 18.9
(3)
P 		13.7
(0.7)
P 		11.2
(-0.4)
P 		5.3
(-3)
P 		14
avr 17.4
(3) 		12.3
(-0.5) 		9.9
(-2.1) 		8.6
(-3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 8.1
(-0.9)
A 		5.7
(-1.6)
A 		5.6
(-1.6)
P 		15.7
(1.5)
P 		20.7
(3)
P 		1.1
(-3)
A 		20
B 20.7
(2.9)
P 		20.8
(3)
P 		14.8
(-0.4)
P 		10.2
(-3)
P 		16.6
(0.6)
P 		13.8
(-1)
P 		11
C 13.9
(2.9)
P 		13.3
(2.2)
A 		9.7
(-1.7)
A 		8.5
(-3)
A 		12.3
(1.1)
A 		14
(3)
P 		6
avr 14.2
(1) 		13.3
(0.2) 		10
(-2.7) 		11.5
(-1.3) 		16.5
(3) 		9.6
(-3) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180221_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15621
Sequence Type Exemplar sequence
Representative Public ID F54E2.5 NCBI
Target Description F54E2.5 /REP_DB=WormBase Gene ID /WP=CE17109 /TR=Q9TXK0 /GB=AAC78201.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071361(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071361 NCBI Caenorhabditis elegans F54E2.5 (F54E2.5) mRNA, complete cds. 11/11 None
GENEFINDER00000004058 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2812358:2818044:-1 11/11 None
F54E2.5 ENSEMBL cdna:known chromosome:CEL140:V:2812358:2815759:-1 gene:F54E2.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2812357-2815759(-) 98.87 100.0
Public Domain and Genome References
Gene Title
Ensembl F54E2.5
Entrez Gene 186244 Entrez gene
SwissProt Q9TXK0 EMBL-EBI
WormBase CE17109 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC78201 Hypothetical protein F54E2.5 [Caenorhabditis elegans] ref|NP_503762.1| F54E2.5 [Caenorhabditis elegans] 0.0
blast AAC78202 Serpentine receptor, class t protein 34 [Caenorhabditis elegans] ref|NP_503763.1| Serpentine Receptor, class T family member (srt-34) [Caenorhabditis elegans] 1.0E-163
blast CAB01181 Hypothetical protein F40G12.8 [Caenorhabditis elegans] ref|NP_506535.2| Serpentine Receptor, class T family member (srt-33) [Caenorhabditis elegans] 1.0E-151
blast AAB88362 Hypothetical protein F58F6.6 [Caenorhabditis elegans] ref|NP_500067.1| F58F6.6 [Caenorhabditis elegans] 7.0E-83
blast AAB52299 Hypothetical protein ZC196.9 [Caenorhabditis elegans] ref|NP_505246.1| ZC196.9 [Caenorhabditis elegans] 3.0E-82
Protein Domains
Database ID Description E-Value
Pfam IPR006874 EMBL-EBI Protein of unknown function DUF621 7.1E-16
Pfam IPR006874 EMBL-EBI Protein of unknown function DUF621 1.2E-64
Pfam IPR006874 EMBL-EBI Protein of unknown function DUF621 2.2E-102
Pfam IPR006874 EMBL-EBI Protein of unknown function DUF621 2.0E-89
Pfam IPR006874 EMBL-EBI Protein of unknown function DUF621 2.2E-102
Pfam IPR002651 EMBL-EBI Protein of unknown function DUF32 1.5E-116
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503762.1 15 32-54,61-83,103-125,145-167,224-246,259-281,296-315,366-383,403-434,441-463,493-515,522-544,587-609,622-644,659-678
F54E2.5 15 32-54,61-83,103-125,145-167,224-246,259-281,296-315,366-383,403-434,441-463,493-515,522-544,587-609,622-644,659-678
GENEFINDER00000004058 23 40-62,75-97,112-134,155-177,203-225,246-265,275-297,320-342,388-410,417-439,459-481,501-523,564-586,599-621,636-655,706-723,743-774,781-803,833-855,862-884,927-949,962-984,999-1018
Sequence
Target Sequence 
>C. ELEGANS:180221_AT
gtcagtttccagctgttttcctggtattaaatatatttttaaattttagctcaataatct
acatttggctgatgtcagtgtgggtgttggctctgattgtggaaattattttgataactt
caaattgcaaatatcggtatgaaaacaagtcaaaaatatatcaactgagatgtgaagcaa
gaggtgaaaccaaacttgttgtcaataaatctctgcccggtggaattcagttgatagaaa
atatagtgcaaattggtattcctattttaatatttgtgatctatattgcaattatttcaa
aaattgcatatatgaagcagagctcattgagcagcactgagatatccattttgaagcaat
cgatttttgtttttgtcgcttttcaggcttcatctgttgtgtttttatttgctcaaaact
ttgaaataacaaatgtcaccgcgttccttgtcaagcgattcgtcaatacaatggaaatac
tagccggagccgcaactcccactttcttttttctcacttctcgggaaatacgaaaattcg
tgttcacgcgagtttcgcctgccggttcacaagggacatctaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCAGTTTCCAGCTGTTTTCCTGGT 59 467 1529 Antisense
GGGTGTTGGCTCTGATTGTGGAAAT 465 497 1610 Antisense
GAGCAGCACTGAGATATCCATTTTG 45 391 1857 Antisense
TTTTTGTCGCTTTTCAGGCTTCATC 582 673 1898 Antisense
AGGCTTCATCTGTTGTGTTTTTATT 197 55 1913 Antisense
AATAACAAATGTCACCGCGTTCCTT 665 169 1953 Antisense
CGCGTTCCTTGTCAAGCGATTCGTC 48 257 1968 Antisense
GAAATACTAGCCGGAGCCGCAACTC 386 363 2002 Antisense
TCTTTTTTCTCACTTCTCGGGAAAT 116 613 2033 Antisense
AATTCGTGTTCACGCGAGTTTCGCC 275 175 2063 Antisense
CTGCCGGTTCACAAGGGACATCTAA 419 237 2087 Antisense
Sequence Source Affymetrix Proprietary Database