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[180217_at] G-protein coupled receptor, srh family

Description Y59A8B.3 /REP_DB=WormBase Gene ID /WP=CE26213 /TR=Q9GRY6 /GB=CAC14413.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 21      4grp_total > 26      6grp_cgc > 61      6grp_cgc_sub > De      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 105
1 2 3 4 range
A 92.2
(-1)
A 		129.6
(2)
A 		68.2
(-3)
A 		141.3
(3)
A 		73
B 79.3
(3)
A 		68
(0.7)
A 		69.3
(0.9)
A 		50.1
(-3)
A 		29
C 36.7
(-3)
A 		89.4
(0)
A 		69.1
(-1.2)
A 		142.1
(3)
A 		105
avr 69.4
(-2.9) 		95.7
(0.8) 		68.9
(-3) 		111.2
(3) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 146
1 2 3 4 5 6 range
A 67.4
(0.6)
A 		53
(-1)
A 		88.4
(3)
A 		34.9
(-3)
A 		76
(1.6)
A 		34.9
(-3)
A 		54
B 84.6
(3)
A 		65
(1.4)
A 		74.5
(2.2)
A 		32.4
(-1.1)
A 		41.1
(-0.4)
A 		8.8
(-3)
A 		76
C 55.9
(-1.7)
A 		155
(3)
A 		68
(-1.1)
A 		101.1
(0.4)
A 		67.8
(-1.1)
A 		28.8
(-3)
A 		126
avr 69.3
(1.1) 		91
(3) 		77
(1.7) 		56.1
(-0.1) 		61.6
(0.4) 		24.2
(-3) 		67
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 33.6
(3)
A 		28.1
(0.9)
A 		17.8
(-3)
A 		20.7
(-1.9)
A 		16
B 25.3
(3)
A 		16.8
(-0.5)
A 		16.1
(-0.8)
A 		10.7
(-3)
A 		15
C 12.1
(-3)
A 		25.7
(3)
A 		24.1
(2.3)
A 		24
(2.3)
A 		14
avr 23.7
(3) 		23.5
(2.8) 		19.3
(-2.1) 		18.5
(-3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 12.4
(-1.4)
A 		18.8
(1.3)
A 		19.3
(1.5)
A 		8.9
(-2.8)
A 		22.9
(3)
A 		8.5
(-3)
A 		14
B 29.4
(3)
A 		21.6
(1.2)
A 		18.1
(0.4)
A 		8.6
(-1.8)
A 		16.7
(0.1)
A 		3.3
(-3)
A 		26
C 20.7
(-1.2)
A 		47.3
(3)
A 		17.3
(-1.8)
A 		20.3
(-1.3)
A 		21.9
(-1.1)
A 		9.7
(-3)
A 		38
avr 20.8
(0.7) 		29.2
(3) 		18.2
(0) 		12.6
(-1.5) 		20.5
(0.6) 		7.2
(-3) 		22
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180217_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14446
Sequence Type Exemplar sequence
Representative Public ID Y59A8B.3 NCBI
Target Description Y59A8B.3 /REP_DB=WormBase Gene ID /WP=CE26213 /TR=Q9GRY6 /GB=CAC14413.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075121(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075121 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-133) (srh-133) mRNA, complete cds. 11/11 None
SNAP00000028698 ENSEMBL cdna:SNAP chromosome:CEL140:V:18014987:18016790:-1 11/11 None
GENEFINDER00000028733 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:18014987:18022456:-1 11/11 None
Y59A8B.3 ENSEMBL cdna:known chromosome:CEL140:V:18014987:18016790:-1 gene:Y59A8B.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:18014986-18016790(-) 100.0 100.0
Public Domain and Genome References
Gene Title G-protein coupled receptor, srh family
Gene Symbol srh-133
Ensembl Y59A8B.3
Entrez Gene 190391 Entrez gene
SwissProt Q9GRY6 EMBL-EBI
WormBase CE26213 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAC14413 Hypothetical protein Y59A8B.3 [Caenorhabditis elegans] ref|NP_507522.1| Serpentine Receptor, class H family member (srh-133) [Caenorhabditis elegans] 0.0
blast CAC14412 Hypothetical protein Y59A8B.4 [Caenorhabditis elegans] ref|NP_507523.1| Serpentine Receptor, class H family member (srh-134) [Caenorhabditis elegans] 1.0E-144
blast CAB04866 Hypothetical protein T27C5.5 [Caenorhabditis elegans] ref|NP_507404.1| Serpentine Receptor, class H family member (srh-132) [Caenorhabditis elegans] 1.0E-125
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 2.1E-27
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.1E-61
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 8.0E-32
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 3.5E-18
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507522.1 7 15-37,58-80,95-117,138-157,194-216,240-262,277-296
Y59A8B.3 7 15-37,58-80,95-117,138-157,194-216,240-262,277-296
SNAP00000028698 7 15-37,58-80,95-117,138-157,194-216,240-262,277-296
GENEFINDER00000028733 15 20-39,46-68,88-110,131-153,168-190,236-258,273-295,315-337,413-435,448-470,490-512,533-555,590-612,636-658,673-692
Sequence
Target Sequence 
>C. ELEGANS:180217_AT
tatggattcactttcacacttcccttacttatacacccaccggatcagatagaagcaagg
aaaatggctaaagaggcgcttccatgtgttcctcactccatcattgatcacactgaattc
tatgtctttgtggaagaaccctacatattcgctatttactatggaattactgcagctgtc
cttacttcacaagccgtttactattttgcacgtactgcattatacctatcaagtaacaaa
gccaaatctcaaaggactcataagcttcaagtacaattttttattgcattaacatttcaa
attgcaatcccattgtgtgttgtaatattgccagttgcttacatcatcactgcatttata
acgaaacattttgatcagatcgccaacaacattgccttaaatttcattgcaatccacggg
ctggtatccagtacagtaatgcttattgcacataaaccatatcgagaagctgttggaaat
atattccggctccgttgcacatgggaacggtgtcatagaagaccactaaattccatcagc
gatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATGGATTCACTTTCACACTTCCCT 657 655 451 Antisense
TTACTTATACACCCACCGGATCAGA 116 683 475 Antisense
AAAGAGGCGCTTCCATGTGTTCCTC 379 123 520 Antisense
TTCCTCACTCCATCATTGATCACAC 613 699 539 Antisense
GGAAGAACCCTACATATTCGCTATT 513 527 582 Antisense
GGAATTACTGCAGCTGTCCTTACTT 480 533 613 Antisense
TCCTTACTTCACAAGCCGTTTACTA 129 591 629 Antisense
TATTGCCAGTTGCTTACATCATCAC 610 663 776 Antisense
TGCAATCCACGGGCTGGTATCCAGT 680 575 858 Antisense
AAATATATTCCGGCTCCGTTGCACA 132 115 927 Antisense
AGACCACTAAATTCCATCAGCGATC 223 71 970 Antisense
Sequence Source Affymetrix Proprietary Database