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[180211_at]

Description F14H3.12 /REP_DB=WormBase Gene ID /WP=CE18594 /TR=O45370 /GB=CAB05490.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 63      6grp_total > 63      4grp_total > 26      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 59
1 2 3 4 range
A 28.8
(-3)
A 		59.7
(1.1)
A 		31.8
(-2.6)
A 		74
(3)
A 		45
B 15.3
(-3)
A 		25.1
(-1.5)
A 		27.2
(-1.2)
A 		55.7
(3)
A 		40
C 26.1
(-3)
A 		52.5
(1.4)
P 		29.6
(-2.4)
A 		62.2
(3)
P 		36
avr 23.4
(-3) 		45.8
(0.3) 		29.5
(-2.1) 		64
(3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 169
1 2 3 4 5 6 range
A 46.7
(-3)
A 		58.2
(-2.3)
P 		150
(3)
P 		81.6
(-1)
P 		88.5
(-0.6)
P 		61
(-2.2)
P 		103
B 32.3
(-3)
P 		52
(-2.3)
P 		200.8
(3)
P 		95.8
(-0.7)
P 		127
(0.4)
P 		78.6
(-1.4)
P 		169
C 32.7
(-3)
P 		90.2
(3)
P 		67.1
(0.6)
P 		84.2
(2.4)
P 		49.4
(-1.3)
P 		64.8
(0.3)
P 		58
avr 37.2
(-3) 		66.8
(-1.3) 		139.3
(3) 		87.2
(-0.1) 		88.3
(0) 		68.1
(-1.2) 		102
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 10.5
(-0.2)
A 		13
(3)
A 		8.3
(-3)
A 		10.8
(0.2)
A 		5
B 4.9
(-3)
A 		6.2
(-1.9)
A 		6.3
(-1.8)
A 		11.9
(3)
A 		7
C 8.6
(-3)
A 		15.1
(3)
P 		10.3
(-1.4)
A 		10.5
(-1.2)
P 		7
avr 8
(-3) 		11.4
(3) 		8.3
(-2.5) 		11.1
(2.5) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 43
1 2 3 4 5 6 range
A 8.6
(-3)
A 		20.7
(0)
P 		32.7
(3)
P 		20.7
(0)
P 		26.7
(1.5)
P 		14.9
(-1.4)
P 		24
B 11.2
(-3)
P 		17.3
(-2.1)
P 		48.8
(2.6)
P 		25.4
(-0.9)
P 		51.6
(3)
P 		29.3
(-0.3)
P 		40
C 12.1
(-3)
P 		27.5
(3)
P 		17.1
(-1.1)
P 		16.9
(-1.1)
P 		16
(-1.5)
P 		21.7
(0.7)
P 		15
avr 10.6
(-3) 		21.8
(0) 		32.9
(3) 		21
(-0.2) 		31.4
(2.6) 		22
(0.1) 		22
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180211_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13642
Sequence Type Exemplar sequence
Representative Public ID F14H3.12 NCBI
Target Description F14H3.12 /REP_DB=WormBase Gene ID /WP=CE18594 /TR=O45370 /GB=CAB05490.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074646(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074646 NCBI Caenorhabditis elegans F14H3.12 (F14H3.12) mRNA, complete cds. 11/11 None
SNAP00000013830 ENSEMBL cdna:SNAP chromosome:CEL140:V:16076744:16077886:-1 10/11 None
F14H3.12 ENSEMBL cdna:known chromosome:CEL140:V:16076744:16077886:-1 gene:F14H3.12 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000013840 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16076743-16077886(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F14H3.12
Entrez Gene 184497 Entrez gene
SwissProt O45370 EMBL-EBI
WormBase CE35483 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB05490 Hypothetical protein F14H3.12 [Caenorhabditis elegans] ref|NP_507047.2| F14H3.12 [Caenorhabditis elegans] 2.0E-77
blast CAB05490 Hypothetical protein F14H3.12 [Caenorhabditis elegans] ref|NP_507047.2| F14H3.12 [Caenorhabditis elegans] 2.0E-71
blast CAB60401 Hypothetical protein Y60A3A.16 [Caenorhabditis elegans] ref|NP_507856.1| Y60A3A.16 [Caenorhabditis elegans] 1.0E-70
blast CAB60401 Hypothetical protein Y60A3A.16 [Caenorhabditis elegans] ref|NP_507856.1| Y60A3A.16 [Caenorhabditis elegans] 4.0E-65
blast CAB54179 Hypothetical protein H39E23.1a [Caenorhabditis elegans] emb|CAB54263.1| Hypothetical protein H39E23.1a [Caenorhabditis elegans] ref|NP_001024018.1| abnormal embryonic PARtitioning of cytoplasm family member (par-1) [Caenorhabditis elegans] 3.0E-33
blast CAB54179 Hypothetical protein H39E23.1a [Caenorhabditis elegans] emb|CAB54263.1| Hypothetical protein H39E23.1a [Caenorhabditis elegans] ref|NP_001024018.1| abnormal embryonic PARtitioning of cytoplasm family member (par-1) [Caenorhabditis elegans] 1.0E-28
Protein Domains
Database ID Description E-Value
Pfam IPR001772 EMBL-EBI Kinase-associated, C-terminal 3.3E-23
Pfam IPR001772 EMBL-EBI Kinase-associated, C-terminal 1.2E-15
Sequence
Target Sequence 
>C. ELEGANS:180211_AT
ggaaccagttgaagagtgcgatgaaaatgttggagcattccaatggcagcaagcgtcaga
gactttgactgcaattcgtcagcggtttgggtttgcgctggagactgctgatcaggaaga
tcaggcgcgagcagtgaggtttacttggagtctgaagaaaacgtcgatagaaattcaaaa
agttcttgaaagctatggcattgattatgaacaacaaaaacgataccttcttcggtgcag
tcatgtggatcctctcactgatgcttcagtaaagtgggatatcgaggtctgcacactacc
acgcttgtatttgaatggagttcattttcaacgaatttcgggaagctcgagcgacttcaa
gaacatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAACCAGTTGAAGAGTGCGATGAA 286 531 18 Antisense
ATGGCAGCAAGCGTCAGAGACTTTG 360 53 60 Antisense
GACTTTGACTGCAATTCGTCAGCGG 149 371 78 Antisense
ATCAGGAAGATCAGGCGCGAGCAGT 463 27 128 Antisense
GGCGCGAGCAGTGAGGTTTACTTGG 418 541 141 Antisense
AAGTTCTTGAAAGCTATGGCATTGA 551 155 197 Antisense
AAAACGATACCTTCTTCGGTGCAGT 203 131 234 Antisense
TCTTCGGTGCAGTCATGTGGATCCT 201 617 246 Antisense
GCTTCAGTAAAGTGGGATATCGAGG 354 295 280 Antisense
CTACCACGCTTGTATTTGAATGGAG 664 225 313 Antisense
AAGCTCGAGCGACTTCAAGAACATT 511 149 360 Antisense
Sequence Source Affymetrix Proprietary Database