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[180206_s_at]

Description C27A7.5B /REP_DB=WormBase Gene ID /WP=CE20528 /TR=P90759 /GB=CAB02789.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 17      4grp_total > 22      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 100
1 2 3 4 range
A 94.2
(3)
A 		35.3
(-1.1)
A 		7.1
(-3)
A 		91.5
(2.8)
A 		87
B 70.6
(3)
M 		16.6
(-2.5)
A 		11.7
(-3)
A 		65.6
(2.5)
A 		59
C 100.6
(2.6)
A 		17.2
(-3)
A 		23.2
(-2.6)
A 		106.6
(3)
A 		89
avr 88.5
(3) 		23
(-2.3) 		14
(-3) 		87.9
(3) 		75

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 95
1 2 3 4 5 6 range
A 56
(-0.1)
A 		47.5
(-0.7)
A 		102
(3)
M 		64
(0.4)
A 		14
(-3)
A 		26.2
(-2.2)
A 		88
B 99.7
(3)
A 		80.5
(1.8)
A 		45.8
(-0.5)
A 		7.3
(-3)
A 		64.1
(0.7)
A 		37
(-1.1)
A 		92
C 60.7
(0.8)
A 		43.2
(-0.7)
A 		15.2
(-3)
A 		42.4
(-0.7)
A 		57.8
(0.6)
A 		86.8
(3)
A 		72
avr 72.1
(3) 		57.1
(0.4) 		54.3
(-0.1) 		37.9
(-3) 		45.3
(-1.7) 		50
(-0.9) 		34
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 32
1 2 3 4 range
A 34.3
(3)
A 		7.7
(-1.9)
A 		1.9
(-3)
A 		13.4
(-0.9)
A 		32
B 22.5
(3)
M 		4.1
(-2.6)
A 		2.7
(-3)
A 		14
(0.4)
A 		20
C 33.3
(3)
A 		4.9
(-3)
A 		8.1
(-2.3)
A 		18
(-0.2)
A 		28
avr 30
(3) 		5.6
(-2.7) 		4.2
(-3) 		15.1
(-0.5) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 10.3
(-1)
A 		16.9
(1.2)
A 		22.2
(3)
M 		16.2
(1)
A 		4.2
(-3)
A 		6.4
(-2.3)
A 		18
B 34.7
(3)
A 		26.8
(1.6)
A 		11.1
(-1.3)
A 		1.9
(-3)
A 		26
(1.4)
A 		13.8
(-0.8)
A 		33
C 22.5
(1.4)
A 		13.2
(-0.8)
A 		3.9
(-3)
A 		8.5
(-1.9)
A 		18.7
(0.5)
A 		29.1
(3)
A 		25
avr 22.5
(3) 		19
(1.5) 		12.4
(-1.5) 		8.9
(-3) 		16.3
(0.3) 		16.4
(0.3) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180206_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12959
Sequence Type Exemplar sequence
Representative Public ID C27A7.5B NCBI
Target Description C27A7.5B /REP_DB=WormBase Gene ID /WP=CE20528 /TR=P90759 /GB=CAB02789.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073648(11), NM_073647(11), NM_073649(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073648 NCBI Caenorhabditis elegans C27A7.5a (asparaginase) mRNA, complete cds. 11/11 A 
NM_073647 NCBI Caenorhabditis elegans C27A7.5c (asparaginase) mRNA, complete cds. 11/11 A 
NM_073649 NCBI Caenorhabditis elegans C27A7.5b (asparaginase) mRNA, complete cds. 11/11 None
C27A7.5a ENSEMBL cdna:known chromosome:CEL140:V:12157465:12164887:-1 gene:C27A7.5 11/11 A 
C27A7.5c ENSEMBL cdna:known chromosome:CEL140:V:12157615:12164886:-1 gene:C27A7.5 11/11 A 
C27A7.5b ENSEMBL cdna:known chromosome:CEL140:V:12162006:12164885:-1 gene:C27A7.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12162193-12164885(-) 100.0 100.0
Public Domain and Genome References
Ensembl C27A7.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB02791 Hypothetical protein C27A7.5a [Caenorhabditis elegans] ref|NP_506049.1| C27A7.5a [Caenorhabditis elegans] 0.0
blast CAB02790 Hypothetical protein C27A7.5c [Caenorhabditis elegans] ref|NP_506048.1| C27A7.5c [Caenorhabditis elegans] 0.0
blast CAE75404 Hypothetical protein CBG23394 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006034 EMBL-EBI Asparaginase/glutaminase 7.7E-55
Pfam IPR002110 EMBL-EBI Ankyrin 10.0
Pfam IPR002110 EMBL-EBI Ankyrin 5.7E-8
Pfam IPR002110 EMBL-EBI Ankyrin 1.2E-8
Pfam IPR006034 EMBL-EBI Asparaginase/glutaminase 7.7E-55
Pfam IPR002110 EMBL-EBI Ankyrin 10.0
Pfam IPR002110 EMBL-EBI Ankyrin 5.7E-8
Pfam IPR002110 EMBL-EBI Ankyrin 1.2E-8
Sequence
Target Sequence 
>C. ELEGANS:180206_S_AT
gatctactcgtcactgacacgtggatttgtaatgcgattattccagggagcagcatataa
accaaaaagtgcctttatcaaaaagatgagtgatggaacgtgtccaccaatcaatattcg
aagatgttcgtgcacaagtgatggactttcagtctcaccgaagaaatcactgacatctcc
aatttgggaccgtcgaccttcgaaaattctagatgaaaaccatttaccaaaacaacttct
cgtccaacatcatcacgaagatggcgcgtttcttcgaaatatttcag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCTACTCGTCACTGACACGTGGA 427 419 15 Antisense
AATGCGATTATTCCAGGGAGCAGCA 378 167 45 Antisense
GAGTGATGGAACGTGTCCACCAATC 478 401 102 Antisense
GAAGATGTTCGTGCACAAGTGATGG 498 337 134 Antisense
GAAGAAATCACTGACATCTCCAATT 614 339 174 Antisense
GACATCTCCAATTTGGGACCGTCGA 526 369 186 Antisense
GACCGTCGACCTTCGAAAATTCTAG 87 381 202 Antisense
TACCAAAACAACTTCTCGTCCAACA 18 649 239 Antisense
ACTTCTCGTCCAACATCATCACGAA 691 103 249 Antisense
TCATCACGAAGATGGCGCGTTTCTT 286 619 264 Antisense
GGCGCGTTTCTTCGAAATATTTCAG 509 541 277 Antisense
Sequence Source Affymetrix Proprietary Database