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[180195_at]

Description T22F3.2 /REP_DB=WormBase Gene ID /WP=CE13964 /TR=Q94301 /GB=AAB18307.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 82      6grp_high1_10_10 > 29      4grp_highest_8_8 > 23      4grp_high_8_8 > 06      6grp_highest_nonorm > 73      6grp_total > 38      4grp_total > 03      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2216
1 2 3 4 range
A 568.6
(-2.5)
P 		368
(-3)
P 		430.8
(-2.8)
P 		2583.6
(3)
P 		2216
B 601.5
(-2.3)
P 		417.5
(-3)
P 		417.3
(-3)
P 		2047
(3)
P 		1630
C 608.3
(-2.7)
P 		553.8
(-2.9)
P 		531.1
(-3)
P 		2288.5
(3)
P 		1757
avr 592.8
(-2.5) 		446.4
(-3) 		459.7
(-3) 		2306.4
(3) 		1860

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 474
1 2 3 4 5 6 range
A 518.2
(-1.9)
P 		607.1
(0.9)
P 		482.2
(-3)
P 		487.9
(-2.8)
P 		518.1
(-1.9)
P 		672.8
(3)
P 		191
B 518
(3)
P 		515.2
(2.9)
P 		348.1
(-3)
P 		433.1
(0)
P 		503.9
(2.5)
P 		510.7
(2.7)
P 		170
C 822.3
(3)
P 		518.9
(-1.9)
P 		501.9
(-2.2)
P 		452.7
(-3)
P 		515.4
(-2)
P 		471
(-2.7)
P 		370
avr 619.5
(3) 		547.1
(0.5) 		444.1
(-3) 		457.9
(-2.5) 		512.5
(-0.7) 		551.5
(0.7) 		175
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 356
1 2 3 4 range
A 207
(-0.4)
P 		79.9
(-3)
P 		112.6
(-2.3)
P 		378.2
(3)
P 		298
B 191.8
(-1.3)
P 		103.1
(-2.9)
P 		96.7
(-3)
P 		436.2
(3)
P 		340
C 201.4
(-1.9)
P 		159.2
(-3)
P 		185.1
(-2.3)
P 		385.9
(3)
P 		227
avr 200.1
(-1.2) 		114.1
(-3) 		131.5
(-2.6) 		400.1
(3) 		286

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 220
1 2 3 4 5 6 range
A 95.5
(-3)
P 		215.9
(3)
P 		105
(-2.5)
P 		123.8
(-1.6)
P 		156.3
(0)
P 		163.8
(0.4)
P 		120
B 180.2
(1.8)
P 		171.5
(1.3)
P 		84.5
(-3)
P 		114.7
(-1.5)
P 		204.7
(3)
P 		190.4
(2.3)
P 		120
C 304.8
(3)
P 		158.3
(-1.1)
P 		127.8
(-2)
P 		91
(-3)
P 		166.8
(-0.9)
P 		157.7
(-1.1)
P 		214
avr 193.5
(3) 		181.9
(2.2) 		105.8
(-3) 		109.8
(-2.7) 		175.9
(1.8) 		170.6
(1.4) 		88
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180195_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14835
Sequence Type Exemplar sequence
Representative Public ID T22F3.2 NCBI
Target Description T22F3.2 /REP_DB=WormBase Gene ID /WP=CE13964 /TR=Q94301 /GB=AAB18307.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171442(11), NM_171923(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171442 NCBI Caenorhabditis elegans T22F3.2a (T22F3.2) mRNA, complete cds. 11/11 None
NM_171923 NCBI Caenorhabditis elegans T22F3.2b (T22F3.2) mRNA, complete cds. 11/11 None
GENEFINDER00000034233 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3587171:3588561:1 9/11 None
T22F3.2a ENSEMBL cdna:known chromosome:CEL140:V:3586465:3588806:1 gene:T22F3.2 11/11 None
T22F3.2b ENSEMBL cdna:known chromosome:CEL140:V:3586465:3588561:1 gene:T22F3.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000034224 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3586464-3588561(+) 100.0 100.0
Public Domain and Genome References
Ensembl T22F3.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB18307 Hypothetical protein T22F3.2a [Caenorhabditis elegans] ref|NP_741517.1| T22F3.2a [Caenorhabditis elegans] 0.0
blast AAM45362 Hypothetical protein T22F3.2b [Caenorhabditis elegans] ref|NP_741518.1| T22F3.2b [Caenorhabditis elegans] 0.0
blast CAE58219 Hypothetical protein CBG01315 [Caenorhabditis briggsae] 1.0E-128
Protein Domains
Database ID Description E-Value
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 1.2E-84
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 1.5E-70
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 3.8E-60
Sequence
Target Sequence 
>C. ELEGANS:180195_AT
caatcctcgtctgaaaggccgcaaagtggccgatgatttgttcggatattccataagaaa
tgaagttcaatgccccgggtgcaagaacaagcacgtctactatgagcacaacaccgttat
gacggttcggatgcttagaaaggatccatcaggcaaaagccactccattaaagaactcat
gcaggctctgttcacggaatccaagatattcggctataattgctcaaagtgcaaaaagaa
atccgacgcgccagtgaagcccacacttctccgagctccacagatcctgctaatgcacat
ttctcgcttctcgtttgatggttgggggaagaaaatctctcggcctgtcaccttaaatga
aactctcgatgtgacgagtatggccaccgccggcgcatccaccgtctataagctgtgcgg
agctgtgatccatggtgggactacgctggactacggccactattggtgtgtggctcgatc
gagaaaacgagaggagcagttcgtgacgttgaatgattcatcggtctcccaacacacgaa
tccacgagatgctcttcaatcctacattgtgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAATCCTCGTCTGAAAGGCCGCAAA 404 181 516 Antisense
TGAAGTTCAATGCCCCGGGTGCAAG 591 573 576 Antisense
GCACGTCTACTATGAGCACAACACC 392 313 606 Antisense
TCATGCAGGCTCTGTTCACGGAATC 460 619 692 Antisense
GCTCCACAGATCCTGCTAATGCACA 508 297 790 Antisense
GCATCCACCGTCTATAAGCTGTGCG 567 309 910 Antisense
GCGGAGCTGTGATCCATGGTGGGAC 309 293 932 Antisense
GGACTACGGCCACTATTGGTGTGTG 27 525 963 Antisense
TTGAATGATTCATCGGTCTCCCAAC 130 703 1024 Antisense
CACACGAATCCACGAGATGCTCTTC 349 195 1048 Antisense
GATGCTCTTCAATCCTACATTGTGC 491 413 1063 Antisense
Sequence Source Affymetrix Proprietary Database