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[180174_at]

Description F25H2.12 /REP_DB=WormBase Gene ID /WP=CE09657 /TR=Q93574 /GB=CAB02100.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 97      6grp_high1_10_10 > 88      4grp_highest_8_8 > 47      4grp_high_8_8 > 20      6grp_highest_nonorm > 90      6grp_total > 78      4grp_total > 20      6grp_highest_sub > Cu      6grp_high1_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 165
1 2 3 4 range
A 174
(-0.2)
P 		137.2
(-1.6)
P 		101.1
(-3)
P 		255.6
(3)
P 		155
B 135.8
(-0.6)
P 		157.7
(0.5)
P 		90.5
(-3)
P 		204.7
(3)
P 		114
C 165.3
(1.3)
P 		131.5
(-1)
P 		101.3
(-3)
P 		191.4
(3)
P 		90
avr 158.4
(0.1) 		142.1
(-0.8) 		97.6
(-3) 		217.2
(3) 		120

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 121
1 2 3 4 5 6 range
A 139
(-2.9)
P 		137.9
(-3)
P 		138.4
(-3)
P 		159.2
(-1.6)
P 		181.8
(-0)
P 		226.4
(3)
P 		89
B 137.3
(-1.2)
P 		105.3
(-3)
P 		130.1
(-1.6)
P 		119.2
(-2.2)
P 		174.3
(0.8)
P 		214.3
(3)
P 		109
C 165.7
(-0)
P 		139.4
(-1.3)
P 		146
(-1)
P 		106.3
(-3)
P 		176.1
(0.5)
P 		225.3
(3)
P 		119
avr 147.3
(-1.7) 		127.5
(-3) 		138.2
(-2.3) 		128.2
(-3) 		177.4
(0.2) 		222
(3) 		95
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 42
1 2 3 4 range
A 63.3
(3)
P 		29.8
(-2.4)
P 		26.4
(-3)
P 		37.4
(-1.2)
P 		37
B 43.3
(2.9)
P 		38.9
(1.8)
P 		21
(-3)
P 		43.6
(3)
P 		23
C 54.7
(3)
P 		37.8
(-1.5)
P 		35.3
(-2.2)
P 		32.3
(-3)
P 		22
avr 53.8
(3) 		35.5
(-1.2) 		27.6
(-3) 		37.8
(-0.7) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 59
1 2 3 4 5 6 range
A 25.6
(-3)
P 		49
(1.8)
P 		30.1
(-2.1)
P 		40.4
(0)
P 		54.9
(3)
P 		55.1
(3)
P 		30
B 47.8
(-1)
P 		35
(-2.6)
P 		31.6
(-3)
P 		31.6
(-3)
P 		70.8
(1.9)
P 		79.9
(3)
P 		48
C 61.4
(1.4)
P 		42.6
(-0.6)
P 		37.2
(-1.2)
P 		21.4
(-3)
P 		57
(1)
P 		75.4
(3)
P 		54
avr 44.9
(-0.9) 		42.2
(-1.3) 		33
(-2.7) 		31.1
(-3) 		60.9
(1.6) 		70.1
(3) 		39
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180174_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1244
Sequence Type Exemplar sequence
Representative Public ID F25H2.12 NCBI
Target Description F25H2.12 /REP_DB=WormBase Gene ID /WP=CE09657 /TR=Q93574 /GB=CAB02100.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060367(11), NM_182028(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060367 NCBI Caenorhabditis elegans F25H2.12a (F25H2.12) mRNA, complete cds. 11/11 None
NM_182028 NCBI Caenorhabditis elegans F25H2.12b (F25H2.12) mRNA, complete cds. 11/11 None
SNAP00000042626 ENSEMBL cdna:SNAP chromosome:CEL140:I:10578204:10580025:1 9/11 None
GENEFINDER00000042640 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:10578204:10580025:1 11/11 None
F25H2.12a ENSEMBL cdna:known chromosome:CEL140:I:10578200:10580025:1 gene:F25H2.12 11/11 None
F25H2.12b ENSEMBL cdna:known chromosome:CEL140:I:10578200:10580025:1 gene:F25H2.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10578134-10579956(+) 100.0 100.0
Public Domain and Genome References
Ensembl F25H2.12
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB02100 Hypothetical protein F25H2.12a [Caenorhabditis elegans] ref|NP_492768.1| F25H2.12a [Caenorhabditis elegans] 0.0
blast CAD57695 Hypothetical protein F25H2.12b [Caenorhabditis elegans] ref|NP_871828.1| F25H2.12b [Caenorhabditis elegans] 0.0
Sequence
Target Sequence 
>C. ELEGANS:180174_AT
gtgctttttggatgttgttaccacaaagaatttcaatgctttcattttttacgaccagaa
aataatgaaaatgaacgagagatttctgaaaatacgtcttttggaaagtcaacagttttt
ccattgagcaagaaatgggaaaacgtcgaaatagactatttacttcgagaagcagcttgc
cacaacaacgagaatcttgaggaacgatttctgaaacagaagattgatctttcacgatat
gctcgatcatatctggaaaaatggatttggaaagtttccgagttgccagaagatcggaat
atcgggatgtgttcggtaaaatgtattgagaatcagacaacgtttgaagaatatattcga
aaagctatggcaaaccggggaaatcatttactggataaagttttgcagataattccacaa
tctgaactatccgctcatgtctcttctctccgaagctctcagttcgattcgtttgaagtt
ctccgatgcatgtttgctccagcgattgagtcagcaattatcgacgatcgagtggaattg
ttaagggaaaatggaataaattctagagttgttccactctttgaaccttctatttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGCTTTTTGGATGTTGTTACCACA 265 479 820 Antisense
AATGCTTTCATTTTTTACGACCAGA 240 163 854 Antisense
CGAGAAGCAGCTTGCCACAACAACG 340 251 985 Antisense
GATTGATCTTTCACGATATGCTCGA 552 433 1041 Antisense
ACAATCTGAACTATCCGCTCATGTC 85 115 1236 Antisense
TCTCTCCGAAGCTCTCAGTTCGATT 325 609 1264 Antisense
AGTTCGATTCGTTTGAAGTTCTCCG 651 63 1280 Antisense
GAAGTTCTCCGATGCATGTTTGCTC 425 337 1294 Antisense
TGTTTGCTCCAGCGATTGAGTCAGC 186 561 1310 Antisense
GAGTCAGCAATTATCGACGATCGAG 708 397 1327 Antisense
TTCCACTCTTTGAACCTTCTATTTC 383 701 1391 Antisense
Sequence Source Affymetrix Proprietary Database