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[180172_at]

Description R04E5.8 /REP_DB=WormBase Gene ID /WP=CE04800 /TR=Q9GYL4 /GB=AAG00010.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 18      4grp_total > 35      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 56
1 2 3 4 range
A 23.7
(-2.1)
A 		63.4
(3)
A 		17
(-2.9)
A 		16.6
(-3)
A 		47
B 7.9
(-3)
A 		51.2
(3)
A 		21.4
(-1.1)
A 		44.5
(2.1)
A 		43
C 13.7
(-3)
A 		19.7
(0.7)
A 		17.2
(-0.8)
A 		23.4
(3)
A 		10
avr 15.1
(-3) 		44.8
(3) 		18.5
(-2.3) 		28.2
(-0.4) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 74
1 2 3 4 5 6 range
A 80.7
(3)
A 		8.2
(-3)
A 		13.5
(-2.6)
A 		21.8
(-1.9)
A 		10.7
(-2.8)
A 		10.6
(-2.8)
A 		73
B 38.5
(3)
A 		22.9
(0.1)
A 		6.4
(-3)
A 		15.6
(-1.3)
A 		12.3
(-1.9)
A 		8.1
(-2.7)
A 		32
C 17.2
(0.8)
A 		21.6
(2.6)
A 		7.8
(-3)
A 		14.7
(-0.2)
A 		16.4
(0.5)
A 		22.5
(3)
A 		15
avr 45.5
(3) 		17.6
(-1.6) 		9.2
(-3) 		17.4
(-1.6) 		13.1
(-2.4) 		13.7
(-2.3) 		36
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 8.6
(0.3)
A 		13.8
(3)
A 		4.4
(-1.9)
A 		2.4
(-3)
A 		11
B 2.5
(-3)
A 		12.6
(3)
A 		5
(-1.5)
A 		9.5
(1.2)
A 		10
C 4.5
(-1.5)
A 		5.7
(2.1)
A 		6
(3)
A 		4
(-3)
A 		2
avr 5.2
(-2.9) 		10.7
(3) 		5.1
(-3) 		5.3
(-2.8) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 14.9
(3)
A 		2.9
(-2.9)
A 		2.9
(-2.9)
A 		5.5
(-1.6)
A 		3.2
(-2.7)
A 		2.6
(-3)
A 		12
B 13.4
(3)
A 		7.6
(0.1)
A 		1.6
(-3)
A 		4.1
(-1.7)
A 		5
(-1.3)
A 		3
(-2.3)
A 		12
C 6.4
(1.8)
A 		6.6
(2)
A 		2
(-3)
A 		2.9
(-2)
A 		5.3
(0.6)
A 		7.5
(3)
A 		6
avr 11.6
(3) 		5.7
(-0.8) 		2.2
(-3) 		4.2
(-1.7) 		4.5
(-1.5) 		4.4
(-1.6) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180172_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16269
Sequence Type Exemplar sequence
Representative Public ID R04E5.8 NCBI
Target Description R04E5.8 /REP_DB=WormBase Gene ID /WP=CE04800 /TR=Q9GYL4 /GB=AAG00010.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029661(11), NM_001029662(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029661 NCBI Caenorhabditis elegans R04E5.8a (R04E5.8) mRNA, complete cds. 11/11 None
NM_001029662 NCBI Caenorhabditis elegans R04E5.8b (R04E5.8) mRNA, complete cds. 9/11 None
GENEFINDER00000036376 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:8802289:8806390:-1 11/11 None
SNAP00000036365 ENSEMBL cdna:SNAP chromosome:CEL140:X:8802596:8806042:-1 11/11 None
R04E5.8a ENSEMBL cdna:known chromosome:CEL140:X:8801561:8806048:-1 gene:R04E5.8 11/11 A 
R04E5.8b ENSEMBL cdna:known chromosome:CEL140:X:8802507:8803122:-1 gene:R04E5.8 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:8802287-8806041(-) 100.0 100.0
Public Domain and Genome References
Ensembl R04E5.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAG00010 Hypothetical protein R04E5.8a [Caenorhabditis elegans] ref|NP_001024832.1| R04E5.8a [Caenorhabditis elegans] 0.0
blast AAP31431 Hypothetical protein R04E5.8b [Caenorhabditis elegans] ref|NP_001024833.1| R04E5.8b [Caenorhabditis elegans] 3.0E-34
blast AAG00010 Hypothetical protein R04E5.8a [Caenorhabditis elegans] ref|NP_001024832.1| R04E5.8a [Caenorhabditis elegans] 3.0E-34
blast AAP31431 Hypothetical protein R04E5.8b [Caenorhabditis elegans] ref|NP_001024833.1| R04E5.8b [Caenorhabditis elegans] 1.0E-32
Sequence
Target Sequence 
>C. ELEGANS:180172_AT
tcacggagcacctcgtagtcgtaactcgaatggtcgtcaacaaggccaacgtcccatcaa
acaatttcaaggtccaacaagccattacagcaaagatagagaacgtgaaattaatatgca
cttggctcggatggttcgggatcaaattgaacgtgaaggcggtgaagctctggaacccgc
tgaaacagaatacagtcattttgctcatgacacttctggtcgaggacgccatcagcagaa
tgatggttatcgaggtgatcgtcgtcatagatctccaggtaagcacattgcgccggcaag
agcacacaatcgtggacagcatcacaatcgtgggcatcatcatggacctccacgtaatca
taatcaagaccgcaaccgtcatcgcaatcatgacggcaaccgccatcaaaaccaggatcg
cagtcgtcaccacaatcaggaccgtaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCACGGAGCACCTCGTAGTCGTAAC 312 621 2430 Antisense
GCCAACGTCCCATCAAACAATTTCA 35 279 2474 Antisense
AATATGCACTTGGCTCGGATGGTTC 132 173 2542 Antisense
TGAAGCTCTGGAACCCGCTGAAACA 226 575 2592 Antisense
TTTGCTCATGACACTTCTGGTCGAG 277 667 2629 Antisense
TCTGGTCGAGGACGCCATCAGCAGA 605 607 2644 Antisense
GATCTCCAGGTAAGCACATTGCGCC 60 419 2699 Antisense
AATCGTGGGCATCATCATGGACCTC 160 169 2755 Antisense
CAATCATGACGGCAACCGCCATCAA 607 181 2814 Antisense
CAAAACCAGGATCGCAGTCGTCACC 94 191 2836 Antisense
GTCGTCACCACAATCAGGACCGTAA 327 473 2852 Antisense
Sequence Source Affymetrix Proprietary Database