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[180154_at]

Description F49C12.2 /REP_DB=WormBase Gene ID /WP=CE03362 /TR=Q20580 /GB=CAA92507.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 10      4grp_total > 28      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 6.2
(-3)
A 		18.6
(3)
A 		13.8
(0.7)
A 		11
(-0.7)
A 		12
B 15.9
(-0.2)
A 		30.2
(3)
A 		5.4
(-2.6)
A 		3.7
(-3)
A 		27
C 30.9
(1.1)
A 		3
(-3)
A 		3.2
(-3)
A 		43.4
(3)
A 		40
avr 17.7
(2.1) 		17.3
(1.9) 		7.5
(-3) 		19.4
(3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 436
1 2 3 4 5 6 range
A 9.1
(-3)
A 		435
(3)
P 		328.1
(1.5)
P 		251.3
(0.4)
P 		216.1
(-0.1)
P 		48.9
(-2.4)
A 		426
B 17.5
(-3)
A 		442.3
(3)
P 		336.7
(1.5)
P 		119.5
(-1.6)
P 		209.1
(-0.3)
P 		53.3
(-2.5)
A 		425
C 6
(-3)
A 		394.9
(3)
P 		203.1
(0)
P 		252.4
(0.8)
P 		157.4
(-0.7)
P 		73.4
(-2)
A 		389
avr 10.9
(-3) 		424.1
(3) 		289.3
(1) 		207.7
(-0.1) 		194.2
(-0.3) 		58.5
(-2.3) 		413
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 2.3
(-1.3)
A 		4
(3)
A 		3.6
(2)
A 		1.6
(-3)
A 		2
B 5.1
(0.9)
A 		7.5
(3)
A 		1.2
(-2.6)
A 		0.8
(-3)
A 		7
C 10.2
(3)
A 		0.9
(-3)
A 		1.1
(-2.9)
A 		7.3
(1.1)
A 		9
avr 5.9
(3) 		4.1
(0.2) 		2
(-3) 		3.2
(-1.2) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 153
1 2 3 4 5 6 range
A 1.7
(-3)
A 		154.7
(3)
P 		71.5
(-0.3)
P 		63.7
(-0.6)
P 		65.2
(-0.5)
P 		11.9
(-2.6)
A 		153
B 6.1
(-3)
A 		147.2
(3)
P 		81.8
(0.2)
P 		31.7
(-1.9)
P 		85
(0.4)
P 		19.9
(-2.4)
A 		141
C 2.2
(-3)
A 		120.5
(3)
P 		51.7
(-0.5)
P 		50.7
(-0.5)
P 		50.9
(-0.5)
P 		24.6
(-1.9)
A 		118
avr 3.3
(-3) 		140.8
(3) 		68.3
(-0.2) 		48.7
(-1) 		67
(-0.2) 		18.8
(-2.3) 		138
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180154_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8911
Sequence Type Exemplar sequence
Representative Public ID F49C12.2 NCBI
Target Description F49C12.2 /REP_DB=WormBase Gene ID /WP=CE03362 /TR=Q20580 /GB=CAA92507.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069223(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069223 NCBI Caenorhabditis elegans F49C12.2 (F49C12.2) mRNA, complete cds. 11/11 None
SNAP00000032690 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9298001:9299197:-1 11/11 None
F49C12.2 ENSEMBL cdna:known chromosome:CEL140:IV:9298001:9299197:-1 gene:F49C12.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000032714 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9298006-9299203(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F49C12.2
Entrez Gene 186025 Entrez gene
SwissProt Q20580 EMBL-EBI
WormBase CE03362 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92507 Hypothetical protein F49C12.2 [Caenorhabditis elegans] ref|NP_501624.1| F49C12.2 [Caenorhabditis elegans] 1.0E-174
blast CAA92509 Hypothetical protein F49C12.4 [Caenorhabditis elegans] ref|NP_501626.1| F49C12.4 [Caenorhabditis elegans] 1.0E-122
blast CAA99867 Hypothetical protein K01D12.1 [Caenorhabditis elegans] ref|NP_506099.1| K01D12.1 [Caenorhabditis elegans] 8.0E-87
Protein Domains
Database ID Description E-Value
Pfam IPR002485 EMBL-EBI Protein of unknown function DUF13 3.4E-57
Sequence
Target Sequence 
>C. ELEGANS:180154_AT
aattaaatatttcattcctccaaataaagaagttcctatgactatggtaactttgggtat
tggtcatgatgttgatgctgaaaaaacactgaaacaggttttgccaaaaacagagttttt
cggagccgatccgacttcaaaaataaataaggacttgtatgaaaaagacctcggaggtaa
atactttgagtacgctgttggcggtcaaaatggaattcaagaagcttttgttcttataga
tggagcatatagtaaccgaaatgttctgaacattgaagcagacctttttttgagagacga
agtacagaaacaaaaagtcgatattttatggatggacattgaaggacacgagtttccagt
actggagatgctccatcgaggaggcgcatttgacaataaaggtgttaaaatatgtcaaat
taacacggaaatccataaagatttggacaatggtgtcactggagaacaaaatatgttcca
tgaatttgtttggaaattattggaagatggtcgatatgtgatggtcaaaccgtttttggt
ttatttctataaagattttattcgaactactcttattaatgttcacgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATTAAATATTTCATTCCTCCAAAT 468 173 357 Antisense
GTTCCTATGACTATGGTAACTTTGG 582 441 388 Antisense
GTATTGGTCATGATGTTGATGCTGA 64 453 413 Antisense
GGAGCCGATCCGACTTCAAAAATAA 461 521 478 Antisense
CTTTGAGTACGCTGTTGGCGGTCAA 695 217 540 Antisense
GAAGCAGACCTTTTTTTGAGAGACG 303 341 631 Antisense
GAGTTTCCAGTACTGGAGATGCTCC 274 399 706 Antisense
GAGATGCTCCATCGAGGAGGCGCAT 162 395 721 Antisense
GATGGTCGATATGTGATGGTCAAAC 18 409 862 Antisense
AGATTTTATTCGAACTACTCTTATT 68 67 909 Antisense
AACTACTCTTATTAATGTTCACGAT 152 141 921 Antisense
Sequence Source Affymetrix Proprietary Database