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[180150_at]

Description F54E2.1 /REP_DB=WormBase Gene ID /WP=CE17105 /TR=Q9TXK1 /GB=AAC78199.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 07      4grp_highest_8_8 > 09      4grp_high_8_8 > 62      6grp_highest_nonorm > 40      6grp_total > 08      4grp_total > 58      6grp_highest_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3482
1 2 3 4 range
A 1959.2
(-0.9)
P 		3382
(1.8)
P 		4016.2
(3)
P 		884.3
(-3)
P 		3132
B 2159.4
(-1.2)
P 		3581.4
(1.5)
P 		4366
(3)
P 		1205
(-3)
P 		3161
C 2248.3
(-0.2)
P 		3139
(1.7)
P 		3741.9
(3)
P 		944.8
(-3)
P 		2797
avr 2122.3
(-0.8) 		3367.5
(1.7) 		4041.4
(3) 		1011.4
(-3) 		3030

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 725
1 2 3 4 5 6 range
A 748
(3)
P 		604.2
(0.9)
P 		415
(-2)
P 		625.6
(1.2)
P 		345.6
(-3)
P 		528.3
(-0.3)
P 		402
B 1035.1
(3)
P 		430.8
(-2)
P 		457.8
(-1.8)
P 		537.3
(-1.1)
P 		310.5
(-3)
P 		492.8
(-1.5)
P 		725
C 986.3
(3)
P 		514.2
(-1.6)
P 		561.3
(-1.2)
P 		480.2
(-2)
P 		373.3
(-3)
P 		467
(-2.1)
P 		613
avr 923.1
(3) 		516.4
(-1.2) 		478
(-1.6) 		547.7
(-0.9) 		343.1
(-3) 		496
(-1.4) 		580
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1175
1 2 3 4 range
A 713.4
(0.8)
P 		734.1
(0.9)
P 		1049.9
(3)
P 		129.4
(-3)
P 		921
B 688.6
(0.4)
P 		884.5
(2)
P 		1011.7
(3)
P 		256.8
(-3)
P 		755
C 744.2
(0.1)
P 		902.5
(0.9)
P 		1304.2
(3)
P 		159.3
(-3)
P 		1145
avr 715.4
(0.4) 		840.4
(1.2) 		1121.9
(3) 		181.8
(-3) 		940

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 275
1 2 3 4 5 6 range
A 137.9
(-0.7)
P 		214.9
(3)
P 		90.4
(-3)
P 		158.7
(0.3)
P 		104.3
(-2.3)
P 		128.6
(-1.2)
P 		125
B 360.2
(3)
P 		143.4
(-2.2)
P 		111.2
(-3)
P 		142.3
(-2.3)
P 		126.2
(-2.6)
P 		183.8
(-1.3)
P 		249
C 365.6
(3)
P 		156.9
(-1.7)
P 		142.9
(-2)
P 		96.6
(-3)
P 		120.8
(-2.5)
P 		156.3
(-1.7)
P 		269
avr 287.9
(3) 		171.7
(-1) 		114.8
(-3) 		132.5
(-2.4) 		117.1
(-2.9) 		156.2
(-1.6) 		173
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180150_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15508
Sequence Type Exemplar sequence
Representative Public ID F54E2.1 NCBI
Target Description F54E2.1 /REP_DB=WormBase Gene ID /WP=CE17105 /TR=Q9TXK1 /GB=AAC78199.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071360(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071360 NCBI Caenorhabditis elegans F54E2.1 (F54E2.1) mRNA, complete cds. 10/11 None
SNAP00000004057 ENSEMBL cdna:SNAP chromosome:CEL140:V:2810461:2811780:1 10/11 None
GENEFINDER00000004062 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2810461:2811898:1 10/11 None
F54E2.1 ENSEMBL cdna:known chromosome:CEL140:V:2810265:2811798:1 gene:F54E2.1 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2810316-2811780(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F54E2.1
Entrez Gene 178742 Entrez gene
SwissProt Q9TXK1 EMBL-EBI
WormBase CE30806 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC78199 Hypothetical protein F54E2.1 [Caenorhabditis elegans] ref|NP_503761.2| F54E2.1 [Caenorhabditis elegans] 0.0
blast CAE66806 Hypothetical protein CBG12168 [Caenorhabditis briggsae] 7.0E-76
blast CAE66806 Hypothetical protein CBG12168 [Caenorhabditis briggsae] 9.0E-76
blast CAE66806 Hypothetical protein CBG12168 [Caenorhabditis briggsae] 6.0E-75
blast CAE64785 Hypothetical protein CBG09578 [Caenorhabditis briggsae] 9.0E-54
Protein Domains
Database ID Description E-Value
Pfam IPR005071 EMBL-EBI Protein of unknown function DUF274 1.0E-126
Pfam IPR005071 EMBL-EBI Protein of unknown function DUF274 1.0E-126
Pfam IPR005071 EMBL-EBI Protein of unknown function DUF274 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:180150_AT
attttgcgcacgttttccaacaacactcaaaacattttcagatatataccaaccgtcgaa
acgttcgcagttacccttggtatattctacatgaaaaccaccaatgatgtctactttcaa
gttgattcaaggaatattgattttaatggctacagcacacaaaattatcaaactaccgga
tttttcatgaaatctaaagaagttgtcgggcacaatgtgacgatcaactgccttcgcgac
acccgcttcaatggaacaactggtgcaaatataatgggctacatgccaactaaatatgga
aaagttacggtacaaaccaatgacaatacatttgctgactcttttaccgtcaccccagtt
gatggaatcctcggcggcagcgttgacaaaatcggtaaaaatatgacaatatcgtcggtg
gatactgacggtggagagtttttcgtgcagtattatgttgtgcagggagaacaaatttcg
tcaacttatgctcccggagagcccacacctccaacctatgagtcgaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTTTGCGCACGTTTTCCAACAACA 229 13 637 Antisense
TATACCAACCGTCGAAACGTTCGCA 193 651 681 Antisense
ACGTTCGCAGTTACCCTTGGTATAT 114 91 697 Antisense
AAAACCACCAATGATGTCTACTTTC 101 131 730 Antisense
CAATGTGACGATCAACTGCCTTCGC 419 183 849 Antisense
ATACATTTGCTGACTCTTTTACCGT 375 23 962 Antisense
TTACCGTCACCCCAGTTGATGGAAT 248 681 980 Antisense
CTCGGCGGCAGCGTTGACAAAATCG 388 229 1006 Antisense
GAGAGTTTTTCGTGCAGTATTATGT 521 395 1070 Antisense
CAAATTTCGTCAACTTATGCTCCCG 345 193 1108 Antisense
CACACCTCCAACCTATGAGTCGACA 669 193 1140 Antisense
Sequence Source Affymetrix Proprietary Database