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[180149_at]

Description T27C4.2 /REP_DB=WormBase Gene ID /WP=CE18296 /TR=O61904 /GB=AAC17699.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 0      6grp_high1_10_10 > 0      4grp_high_8_8 > 45      6grp_highest_nonorm > 60      6grp_total > 03      4grp_total > 50      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 562
1 2 3 4 range
A 225.9
(-1.7)
P 		449.6
(2.8)
P 		461.1
(3)
P 		158
(-3)
A 		303
B 272.1
(-1.8)
P 		719.5
(3)
P 		410.5
(-0.3)
P 		160.3
(-3)
P 		559
C 239.9
(-3)
P 		595.3
(3)
P 		595.4
(3)
P 		246.6
(-2.9)
M 		356
avr 246
(-2.1) 		588.1
(3) 		489
(1.5) 		188.3
(-3) 		400

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 563
1 2 3 4 5 6 range
A 694.1
(3)
P 		415.9
(-0.1)
P 		267
(-1.8)
P 		393.6
(-0.4)
P 		298.3
(-1.4)
P 		160.1
(-3)
P 		534
B 391.7
(0.5)
P 		376.4
(0.3)
P 		327.6
(-0.6)
P 		535.1
(3)
P 		227.3
(-2.3)
P 		185.9
(-3)
P 		349
C 420.5
(3)
P 		309.9
(0.7)
P 		328.4
(1.1)
P 		368.4
(1.9)
P 		355.2
(1.6)
P 		131.1
(-3)
P 		289
avr 502.1
(3) 		367.4
(0.6) 		307.7
(-0.4) 		432.4
(1.8) 		293.6
(-0.6) 		159
(-3) 		343
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 184
1 2 3 4 range
A 82.3
(0.6)
P 		97.6
(1.6)
P 		120.6
(3)
P 		23.1
(-3)
A 		98
B 86.8
(-0.8)
P 		177.7
(3)
P 		95.1
(-0.5)
P 		34.2
(-3)
P 		144
C 79.4
(-1.6)
P 		171.1
(1.7)
P 		207.5
(3)
P 		41.6
(-3)
M 		166
avr 82.8
(-0.4) 		148.8
(3) 		141.1
(2.6) 		33
(-3) 		116

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 117
1 2 3 4 5 6 range
A 127.9
(1.9)
P 		147.9
(3)
P 		58.1
(-1.9)
P 		99.8
(0.3)
P 		90
(-0.2)
P 		39
(-3)
P 		109
B 136.3
(2.6)
P 		125.3
(1.6)
P 		79.5
(-2.2)
P 		141.7
(3)
P 		92.3
(-1.1)
P 		69.3
(-3)
P 		72
C 155.8
(3)
P 		94.6
(-0.3)
P 		83.6
(-0.9)
P 		74.1
(-1.4)
P 		114.9
(0.8)
P 		43.9
(-3)
P 		112
avr 140
(3) 		122.6
(1.8) 		73.7
(-1.5) 		105.2
(0.7) 		99.1
(0.3) 		50.7
(-3) 		89
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180149_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15896
Sequence Type Exemplar sequence
Representative Public ID T27C4.2 NCBI
Target Description T27C4.2 /REP_DB=WormBase Gene ID /WP=CE18296 /TR=O61904 /GB=AAC17699.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071634(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071634 NCBI Caenorhabditis elegans T27C4.2 (T27C4.2) mRNA, complete cds. 11/11 None
SNAP00000000362 ENSEMBL cdna:SNAP chromosome:CEL140:V:3719667:3731519:-1 11/11 None
GENEFINDER00000000365 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3727297:3730737:-1 11/11 None
T27C4.2 ENSEMBL cdna:known chromosome:CEL140:V:3727297:3731583:-1 gene:T27C4.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3727296-3730737(-) 94.36 94.36
Public Domain and Genome References
Gene Title
Ensembl T27C4.2
Entrez Gene 188979 Entrez gene
SwissProt O61904 EMBL-EBI
WormBase CE18296 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC17699 Hypothetical protein T27C4.2 [Caenorhabditis elegans] ref|NP_504035.1| T27C4.2 [Caenorhabditis elegans] 0.0
blast CAE62556 Hypothetical protein CBG06666 [Caenorhabditis briggsae] 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504035.1 1 560-582
T27C4.2 1 560-582
SNAP00000000362 1 527-549
GENEFINDER00000000365 1 524-546
Sequence
Target Sequence 
>C. ELEGANS:180149_AT
cggaagagtcgatgtgctcgcggagccagatgctaatgacacgaagatagagttcaatga
tacgcaacgcagtaagatagtggtaatgattcagaccgttctcaagtcgtccgtcttctc
cgccgagccgttcattttccacgtaaatcagacaatcaacatgaatagtacatatggaat
gcgattttgtgttttcaattcatcacagattaggagcccccacaactacaactgggacga
tgtgagtcgtttcgagaagaacgagcaaatttctgaagtcatccagttgtcgcctgtcag
atttaccaacaaactcccactacagtacccaaatgtggacacgtattggagtactgtaat
gacgatcgcgaaaatttcgattttggtgattttgggaatcgcgatttttattttggtcta
cctgaattgtaccaagtttcggacaatgtacgataggaaaaggacgcattatttcagacc
gttttatattgatccatcgatccatctgtctagtacagcaccgtcatctcgtggaagtgg
cggcggtggcctgaaacgagat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGGAAGAGTCGATGTGCTCGCGGAG 440 241 1335 Antisense
GATTCAGACCGTTCTCAAGTCGTCC 403 431 1422 Antisense
GCCGTTCATTTTCCACGTAAATCAG 483 281 1461 Antisense
TCATCACAGATTAGGAGCCCCCACA 270 619 1534 Antisense
AGCCCCCACAACTACAACTGGGACG 663 81 1549 Antisense
TCTGAAGTCATCCAGTTGTCGCCTG 356 609 1606 Antisense
TTGTCGCCTGTCAGATTTACCAACA 558 701 1621 Antisense
GCGATTTTTATTTTGGTCTACCTGA 311 289 1735 Antisense
TCCATCGATCCATCTGTCTAGTACA 326 591 1827 Antisense
AGCACCGTCATCTCGTGGAAGTGGC 163 77 1851 Antisense
TGGCGGCGGTGGCCTGAAACGAGAT 196 543 1872 Antisense
Sequence Source Affymetrix Proprietary Database