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[180147_at]

Description C54H2.3 /REP_DB=WormBase Gene ID /WP=CE06985 /TR=Q18861 /GB=AAB00588.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 39      6grp_high1_10_10 > 93      4grp_highest_8_8 > 47      4grp_high_8_8 > 11      6grp_highest_nonorm > 81      6grp_total > 90      4grp_total > 20      6grp_highest_sub > 04      6grp_cgc > 96      6grp_high1_sub > 04      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 175
1 2 3 4 range
A 287.1
(0.1)
P 		259.8
(-1.1)
P 		216.1
(-3)
P 		353.8
(3)
P 		138
B 260.3
(1.2)
P 		218.5
(-0.9)
P 		178.8
(-3)
P 		294.5
(3)
P 		116
C 266.6
(-0.5)
P 		252.2
(-1.3)
P 		221.5
(-3)
P 		331.1
(3)
P 		110
avr 271.3
(0.3) 		243.5
(-1.1) 		205.5
(-3) 		326.5
(3) 		121

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 195
1 2 3 4 5 6 range
A 236.2
(-1.1)
P 		315.7
(1.4)
P 		173.7
(-3)
P 		369.1
(3)
P 		313.5
(1.3)
P 		332.9
(1.9)
P 		195
B 313.6
(3)
P 		211.5
(-3)
P 		213
(-2.9)
P 		294.4
(1.9)
P 		307.5
(2.6)
P 		279.8
(1)
P 		102
C 252.9
(-3)
P 		325.4
(2.5)
P 		331.5
(3)
P 		286.4
(-0.4)
P 		261.6
(-2.3)
P 		286.8
(-0.4)
P 		79
avr 267.6
(-0.8) 		284.2
(0.5) 		239.4
(-3) 		316.6
(3) 		294.2
(1.3) 		299.8
(1.7) 		77
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 63
1 2 3 4 range
A 104.5
(3)
P 		56.4
(-2.5)
P 		56.5
(-2.5)
P 		51.8
(-3)
P 		53
B 83
(3)
P 		54
(-1.2)
P 		41.4
(-3)
P 		62.8
(0.1)
P 		42
C 88.2
(3)
P 		72.5
(0.1)
P 		77.2
(1)
P 		55.8
(-3)
P 		32
avr 91.9
(3) 		61
(-2.3) 		58.4
(-2.7) 		56.8
(-3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 87
1 2 3 4 5 6 range
A 43.5
(-2.5)
P 		112.3
(3)
P 		37.8
(-3)
P 		93.6
(1.5)
P 		94.6
(1.6)
P 		81.1
(0.5)
P 		75
B 109.1
(1.7)
P 		70.4
(-1.5)
P 		51.7
(-3)
P 		78
(-0.8)
P 		124.9
(3)
P 		104.4
(1.3)
P 		73
C 93.7
(2.2)
P 		99.3
(3)
P 		84.4
(0.9)
P 		57.6
(-3)
P 		84.7
(0.9)
P 		96
(2.5)
P 		42
avr 82.1
(0.3) 		94
(2) 		58
(-3) 		76.4
(-0.5) 		101.4
(3) 		93.8
(1.9) 		43
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180147_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16157
Sequence Type Exemplar sequence
Representative Public ID C54H2.3 NCBI
Target Description C54H2.3 /REP_DB=WormBase Gene ID /WP=CE06985 /TR=Q18861 /GB=AAB00588.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076566(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076566 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-294) (tag-294) mRNA, complete cds. 11/11 None
SNAP00000031925 ENSEMBL cdna:SNAP chromosome:CEL140:X:5760795:5763320:-1 11/11 None
GENEFINDER00000031932 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:5760795:5763320:-1 11/11 None
C54H2.3 ENSEMBL cdna:known chromosome:CEL140:X:5760254:5763334:-1 gene:C54H2.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5760794-5763320(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol tag-294
Ensembl C54H2.3
Entrez Gene 180842 Entrez gene
SwissProt Q18861 EMBL-EBI
WormBase CE06985 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_508967 Temporarily Assigned Gene name family member (tag-294) [Caenorhabditis elegans] gb|AAB00588.1| Hypothetical protein C54H2.3 [Caenorhabditis elegans] 1.0E-171
blast CAE68796 Hypothetical protein CBG14746 [Caenorhabditis briggsae] 3.0E-44
Protein Domains
Database ID Description E-Value
Pfam IPR001876 EMBL-EBI Zn-finger, Ran-binding 2.1E-6
Sequence
Target Sequence 
>C. ELEGANS:180147_AT
gacatcagcaccttcagtagtacttttaccagaacctacgcctaaaatatctgtcgctga
aacaatggttgttccgaaagcacttcccgcatctattgcaattccgtcaacttccacagc
ttcagtttcaactcatccaactgaaaaaccgaaagaagactcgatcctccaacatcttcc
agagcgaatcgttaaaaagttgaaccagccttcttccctcaactacgacttgctcaaggt
tcacaaaagaatgaccgggattgatccaatagttgctgaatccgaagccgtgttattgcc
gcaagactggaaccgacagaaagtcatgaaagaactgacagcttcgttcggaggaaatca
agctttaagcccgacaacatccagacaatcatcagaaactgactcgtcgtcttccgaagg
aaacagaagctttgaaacaccgctcaatgatgcttctatcttcaattcgaaaagctgcca
gattccggattatatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACATCAGCACCTTCAGTAGTACTT 209 371 726 Antisense
ACCTACGCCTAAAATATCTGTCGCT 50 87 759 Antisense
ACTTCCACAGCTTCAGTTTCAACTC 439 103 835 Antisense
TCCTCCAACATCTTCCAGAGCGAAT 569 593 890 Antisense
TCCCTCAACTACGACTTGCTCAAGG 442 597 940 Antisense
TGAATCCGAAGCCGTGTTATTGCCG 115 575 1002 Antisense
TATTGCCGCAAGACTGGAACCGACA 520 663 1019 Antisense
TTAAGCCCGACAACATCCAGACAAT 27 683 1090 Antisense
TCAGAAACTGACTCGTCGTCTTCCG 298 623 1117 Antisense
AACACCGCTCAATGATGCTTCTATC 25 137 1161 Antisense
AAGCTGCCAGATTCCGGATTATATG 497 149 1197 Antisense
Sequence Source Affymetrix Proprietary Database