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[180137_s_at]

Description T10B5.6 /REP_DB=WormBase Gene ID /WP=CE18236 /TR=O76406 /GB=AAC19225.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 89      6grp_high1_10_10 > 98      4grp_highest_8_8 > 05      4grp_high_8_8 > 30      6grp_highest_nonorm > 93      6grp_total > 98      4grp_total > 25      6grp_highest_sub > In      6grp_cgc > 69      6grp_high1_sub > In      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 234
1 2 3 4 range
A 281.3
(-3)
P 		327.2
(-1.6)
P 		350.7
(-0.8)
P 		473.1
(3)
P 		192
B 239.4
(-3)
P 		388.2
(1.1)
P 		366.9
(0.5)
P 		455.2
(3)
P 		216
C 324.3
(-3)
P 		376.3
(2.3)
P 		357.3
(0.3)
P 		383.5
(3)
P 		59
avr 281.7
(-3) 		363.9
(0.2) 		358.3
(-0) 		437.3
(3) 		156

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 462
1 2 3 4 5 6 range
A 262.1
(-2.9)
P 		251.2
(-3)
P 		300
(-2.3)
P 		298.1
(-2.4)
P 		604.3
(1.8)
P 		696.5
(3)
P 		445
B 234.1
(-3)
P 		250.3
(-2.8)
P 		245.6
(-2.8)
P 		311
(-2)
P 		502.5
(0.6)
P 		677.4
(3)
P 		443
C 256.5
(-3)
P 		260.9
(-2.9)
P 		286.1
(-2.3)
P 		295.5
(-2.1)
P 		517.1
(3)
P 		508.4
(2.8)
P 		261
avr 250.9
(-3) 		254.1
(-2.9) 		277.2
(-2.6) 		301.5
(-2.2) 		541.3
(1.6) 		627.4
(3) 		377
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 102.4
(3)
P 		71
(-2.7)
P 		91.7
(1.1)
P 		69.3
(-3)
P 		33
B 76.3
(-3)
P 		95.9
(2.7)
P 		85
(-0.5)
P 		97
(3)
P 		21
C 107.4
(1.3)
P 		108.2
(1.4)
P 		124.5
(3)
P 		64.7
(-3)
P 		60
avr 95.4
(1.7) 		91.7
(0.8) 		100.4
(3) 		77
(-3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 204
1 2 3 4 5 6 range
A 48.3
(-3)
P 		89.3
(-1.2)
P 		65.3
(-2.2)
P 		75.6
(-1.8)
P 		182.3
(3)
P 		169.6
(2.4)
P 		134
B 81.5
(-2.3)
P 		83.3
(-2.3)
P 		59.6
(-3)
P 		82.4
(-2.3)
P 		204.1
(1.5)
P 		252.6
(3)
P 		193
C 95.1
(-1.1)
P 		79.6
(-1.9)
P 		72.8
(-2.3)
P 		59.4
(-3)
P 		167.3
(2.8)
P 		170.2
(3)
P 		111
avr 75
(-2.6) 		84.1
(-2.2) 		65.9
(-3) 		72.5
(-2.7) 		184.6
(2.4) 		197.5
(3) 		132
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180137_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15824
Sequence Type Exemplar sequence
Representative Public ID T10B5.6 NCBI
Target Description T10B5.6 /REP_DB=WormBase Gene ID /WP=CE18236 /TR=O76406 /GB=AAC19225.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071119(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071119 NCBI Caenorhabditis elegans Kinetochore NuLl family member (knl-3) (knl-3) mRNA, complete cds. 11/11 None
SNAP00000014351 ENSEMBL cdna:SNAP chromosome:CEL140:V:1863046:1870189:1 11/11 None
GENEFINDER00000014358 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:1869042:1870189:1 11/11 None
T10B5.6 ENSEMBL cdna:known chromosome:CEL140:V:1869042:1870320:1 gene:T10B5.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1869041-1870189(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol knl-3
Ensembl T10B5.6
Entrez Gene 178669 Entrez gene
SwissProt O76406 EMBL-EBI
WormBase CE18236 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC19225 Kinetochore null protein 3 [Caenorhabditis elegans] ref|NP_503520.1| Kinetochore NuLl family member (knl-3) [Caenorhabditis elegans] 1.0E-160
blast AAC19225 Kinetochore null protein 3 [Caenorhabditis elegans] ref|NP_503520.1| Kinetochore NuLl family member (knl-3) [Caenorhabditis elegans] 1.0E-156
blast CAE74143 Hypothetical protein CBG21814 [Caenorhabditis briggsae] 9.0E-44
blast CAE74143 Hypothetical protein CBG21814 [Caenorhabditis briggsae] 1.0E-43
blast CAE74143 Hypothetical protein CBG21814 [Caenorhabditis briggsae] 5.0E-40
blast CAE74144 Hypothetical protein CBG21815 [Caenorhabditis briggsae] 8.0E-17
Sequence
Target Sequence 
>C. ELEGANS:180137_S_AT
gccaacgcctgggaagcgtctgttcttcaccagcaaagacactacgaacgaggatcgcaa
ggctgatgactcgacgcaactggagcttctgatggcaaagattccgcaggagatgtcggg
ttacatgaaaaagttggccaccgacatcaaagagatgagccaatacgatgcacagctggc
gcggaatatcgacgacatggaagcggttttcaaatcccgcgaaactaagctcaagtcagc
tattgaaatcgccaaggacagcttcatctcgtccgctctgcctcttcgcctcgagaccct
ggacatgactctgcgtaaagaagaatcgccaaatgatatcgttgcgagacttcacggaag
ttttctcgacaaaacccaggatgccggaaacgtgaatactgcacaaaaggagaaccagca
gctggaggatgagcgcgggatgctggaagaaaagctggagagtctcaacaagatcaagag
agagaaggatgaatttctggcgaaaatgcgaaaagaggaagaggaagcgctcgctcgccg
ggaggaaatcaagcagggcttcgagcgaatcgatgct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCAACGCCTGGGAAGCGTCTGTTC 353 277 426 Antisense
GCGTCTGTTCTTCACCAGCAAAGAC 107 293 441 Antisense
GATCGCAAGGCTGATGACTCGACGC 415 417 478 Antisense
GACTCGACGCAACTGGAGCTTCTGA 11 375 493 Antisense
TACGATGCACAGCTGGCGCGGAATA 237 647 589 Antisense
CGCCAAGGACAGCTTCATCTCGTCC 192 257 675 Antisense
CCCTGGACATGACTCTGCGTAAAGA 223 259 722 Antisense
GATATCGTTGCGAGACTTCACGGAA 652 425 760 Antisense
TTCTCGACAAAACCCAGGATGCCGG 424 669 788 Antisense
GAGGAAGAGGAAGCGCTCGCTCGCC 411 405 940 Antisense
GCAGGGCTTCGAGCGAATCGATGCT 563 311 978 Antisense
Sequence Source Affymetrix Proprietary Database