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[180135_at]

Description F49C12.3 /REP_DB=WormBase Gene ID /WP=CE03363 /TR=Q20581 /GB=CAA92508.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 10      4grp_total > 19      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 85
1 2 3 4 range
A 3.2
(-3)
A 		32.3
(0)
A 		5.8
(-2.7)
A 		60.9
(3)
A 		58
B 25.4
(0.3)
A 		41.4
(3)
A 		5.9
(-3)
A 		17.4
(-1.1)
A 		36
C 26.3
(-1.6)
A 		6.8
(-3)
A 		26.1
(-1.6)
A 		88.1
(3)
P 		81
avr 18.3
(-2.2) 		26.8
(-1) 		12.6
(-3) 		55.5
(3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 291
1 2 3 4 5 6 range
A 19.9
(-3)
A 		293.9
(3)
P 		232.7
(1.7)
P 		170.5
(0.3)
P 		120.9
(-0.8)
P 		35.7
(-2.7)
A 		274
B 9
(-3)
A 		292.6
(3)
P 		213.1
(1.3)
P 		166.9
(0.3)
P 		135.3
(-0.3)
P 		83
(-1.4)
P 		284
C 3
(-3)
A 		259.4
(3)
P 		112.7
(-0.4)
P 		192.2
(1.4)
P 		149.2
(0.4)
P 		74.4
(-1.3)
P 		256
avr 10.6
(-3) 		282
(3) 		186.2
(0.9) 		176.5
(0.7) 		135.1
(-0.2) 		64.4
(-1.8) 		271
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 1.2
(-3)
A 		7
(1.5)
A 		1.5
(-2.8)
A 		8.9
(3)
A 		8
B 8.1
(1.6)
A 		10.2
(3)
A 		1.4
(-3)
A 		3.7
(-1.4)
A 		9
C 8.7
(0.1)
A 		2
(-3)
A 		9.1
(0.3)
A 		14.9
(3)
P 		13
avr 6
(-0.7) 		6.4
(-0.2) 		4
(-3) 		9.2
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 103
1 2 3 4 5 6 range
A 3.7
(-3)
A 		104.5
(3)
P 		50.7
(-0.2)
P 		43.2
(-0.6)
P 		36.5
(-1)
P 		8.7
(-2.7)
A 		101
B 3.1
(-3)
A 		97.4
(3)
P 		51.7
(0.1)
P 		44.2
(-0.4)
P 		55
(0.3)
P 		30.9
(-1.2)
P 		94
C 1.1
(-3)
A 		79.2
(3)
P 		28.7
(-0.9)
P 		38.7
(-0.1)
P 		48.3
(0.6)
P 		24.9
(-1.2)
P 		78
avr 2.6
(-3) 		93.7
(3) 		43.7
(-0.3) 		42
(-0.4) 		46.6
(-0.1) 		21.5
(-1.8) 		91
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180135_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9103
Sequence Type Exemplar sequence
Representative Public ID F49C12.3 NCBI
Target Description F49C12.3 /REP_DB=WormBase Gene ID /WP=CE03363 /TR=Q20581 /GB=CAA92508.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069224(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069224 NCBI Caenorhabditis elegans F49C12.3 (F49C12.3) mRNA, complete cds. 11/11 None
SNAP00000032691 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9300586:9301928:1 11/11 None
GENEFINDER00000032704 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9300404:9301928:1 11/11 None
F49C12.3 ENSEMBL cdna:known chromosome:CEL140:IV:9300073:9301737:1 gene:F49C12.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9300078-9301743(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F49C12.3
Entrez Gene 186026 Entrez gene
SwissProt Q20581 EMBL-EBI
WormBase CE03363 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92508 Hypothetical protein F49C12.3 [Caenorhabditis elegans] ref|NP_501625.1| F49C12.3 [Caenorhabditis elegans] 0.0
blast CAA92508 Hypothetical protein F49C12.3 [Caenorhabditis elegans] ref|NP_501625.1| F49C12.3 [Caenorhabditis elegans] 1.0E-175
blast CAA92508 Hypothetical protein F49C12.3 [Caenorhabditis elegans] ref|NP_501625.1| F49C12.3 [Caenorhabditis elegans] 1.0E-150
blast CAA92506 Hypothetical protein F49C12.1 [Caenorhabditis elegans] ref|NP_501623.1| F49C12.1 [Caenorhabditis elegans] 1.0E-142
blast CAA92506 Hypothetical protein F49C12.1 [Caenorhabditis elegans] ref|NP_501623.1| F49C12.1 [Caenorhabditis elegans] 1.0E-121
blast CAB07607 Hypothetical protein F28G4.4 [Caenorhabditis elegans] ref|NP_507111.2| F28G4.4 [Caenorhabditis elegans] 1.0E-109
blast CAB07607 Hypothetical protein F28G4.4 [Caenorhabditis elegans] ref|NP_507111.2| F28G4.4 [Caenorhabditis elegans] 1.0E-100
Protein Domains
Database ID Description E-Value
Pfam IPR005069 EMBL-EBI Protein of unknown function DUF271 1.0E-126
Pfam IPR005069 EMBL-EBI Protein of unknown function DUF271 1.0E-126
Pfam IPR005069 EMBL-EBI Protein of unknown function DUF271 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000032704 2 4-21,41-63
Sequence
Target Sequence 
>C. ELEGANS:180135_AT
attcttattgatttgacgccgttgaagaaatcaatagaagctgttcaagagaatcgtgaa
tatttaacatacggtttagtgttgatggttcgagcaagaatatgcgctcatttggcacaa
cgaggagtcaatttctggctcatgcaacaggatacaatatggactgaaaatttacaaatg
tcatatttagaagaaaagtacccagaagctcatttgatcttagatagaattggaaatgaa
atgccatttttctatcgaatgcaaatgtgggcctgtggagctacattttttgtgagagga
agtcctacaacttatcagtattttaaacaagttgaatcatatatgttcactgatcaaagt
cctgattcaacaattatgacttacctttgtggtcataatggatacaaatgcgagtttctg
tcctattcagttgcttcgtctagtaattattttgaaacattacgtgaatacgttcccgcg
ttgattcaacttgacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTCTTATTGATTTGACGCCGTTGA 199 11 454 Antisense
ATGCGCTCATTTGGCACAACGAGGA 365 41 555 Antisense
GAGGAGTCAATTTCTGGCTCATGCA 496 403 575 Antisense
GAAGAAAAGTACCCAGAAGCTCATT 374 339 643 Antisense
GAAATGCCATTTTTCTATCGAATGC 593 361 691 Antisense
TGCAAATGTGGGCCTGTGGAGCTAC 699 575 713 Antisense
ATATGTTCACTGATCAAAGTCCTGA 567 21 794 Antisense
ACAAATGCGAGTTTCTGTCCTATTC 595 111 857 Antisense
GTTTCTGTCCTATTCAGTTGCTTCG 287 451 867 Antisense
ACATTACGTGAATACGTTCCCGCGT 580 101 910 Antisense
GTTCCCGCGTTGATTCAACTTGACG 677 441 925 Antisense
Sequence Source Affymetrix Proprietary Database