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[180129_at]

Description T06A4.2 /REP_DB=WormBase Gene ID /WP=CE18211 /TR=Q9TZH3 /GB=AAC67472.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 05      6grp_cgc > 79      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 105
1 2 3 4 range
A 50
(-1.5)
A 		27.4
(-3)
A 		61.2
(-0.8)
A 		120.3
(3)
A 		93
B 34
(-1.3)
A 		69.7
(3)
A 		19.3
(-3)
A 		62.7
(2.2)
A 		50
C 77.1
(3)
A 		15.1
(-3)
A 		34.4
(-1.1)
A 		34.3
(-1.1)
A 		62
avr 53.7
(-0.2) 		37.4
(-3) 		38.3
(-2.8) 		72.4
(3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 155
1 2 3 4 5 6 range
A 93.8
(-0)
A 		46.1
(-1.9)
A 		18.1
(-3)
A 		24.8
(-2.7)
A 		138.5
(1.7)
A 		170.4
(3)
A 		152
B 24.7
(-3)
A 		43.3
(-1.7)
A 		58.2
(-0.6)
A 		45.7
(-1.5)
A 		105.6
(2.8)
A 		109.1
(3)
A 		84
C 15.1
(-3)
A 		50.5
(-1)
A 		79.6
(0.6)
A 		99.8
(1.7)
A 		123.8
(3)
A 		106
(2)
A 		109
avr 44.5
(-3) 		46.6
(-2.9) 		52
(-2.5) 		56.8
(-2.1) 		122.6
(2.6) 		128.5
(3) 		84
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 18.2
(3)
A 		5.9
(-3)
A 		16
(1.9)
A 		17.6
(2.7)
A 		12
B 10.8
(-0)
A 		17.2
(3)
A 		4.5
(-3)
A 		13.4
(1.2)
A 		13
C 25.5
(3)
A 		4.3
(-3)
A 		12
(-0.8)
A 		5.8
(-2.6)
A 		21
avr 18.2
(3) 		9.1
(-3) 		10.8
(-1.9) 		12.3
(-0.9) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 39
1 2 3 4 5 6 range
A 17.3
(-0.9)
A 		16.4
(-1)
A 		3.9
(-3)
A 		6.3
(-2.6)
A 		41.8
(3)
A 		41.5
(3)
A 		38
B 8.6
(-3)
A 		14.4
(-2)
A 		14.1
(-2)
A 		12.1
(-2.4)
A 		42.9
(3)
A 		40.7
(2.6)
A 		34
C 5.6
(-3)
A 		15.4
(-1.3)
A 		20.2
(-0.5)
A 		20.1
(-0.5)
A 		40.1
(3)
A 		35.5
(2.2)
A 		35
avr 10.5
(-3) 		15.4
(-2.1) 		12.7
(-2.6) 		12.8
(-2.6) 		41.6
(3) 		39.2
(2.5) 		31
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180129_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2
Sequence Type Exemplar sequence
Representative Public ID T06A4.2 NCBI
Target Description T06A4.2 /REP_DB=WormBase Gene ID /WP=CE18211 /TR=Q9TZH3 /GB=AAC67472.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058377(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058377 NCBI Caenorhabditis elegans T06A4.2 (T06A4.2) mRNA, complete cds. 11/11 None
SNAP00000004749 ENSEMBL cdna:SNAP chromosome:CEL140:I:759823:761838:-1 11/11 None
GENEFINDER00000004755 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:760951:762461:-1 10/11 None
T06A4.2 ENSEMBL cdna:known chromosome:CEL140:I:760919:761838:-1 gene:T06A4.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:760916-761838(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol mps-3
Ensembl T06A4.2
Entrez Gene 188157 Entrez gene
SwissProt Q9TZH3 EMBL-EBI
WormBase CE29464 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_490778 T06A4.2 [Caenorhabditis elegans] gb|AAQ96605.1| MiRP5 [Caenorhabditis elegans] gb|AAC67472.2| Hypothetical protein T06A4.2 [Caenorhabditis elegans] 2.0E-24
blast NP_490778 T06A4.2 [Caenorhabditis elegans] gb|AAQ96605.1| MiRP5 [Caenorhabditis elegans] gb|AAC67472.2| Hypothetical protein T06A4.2 [Caenorhabditis elegans] 8.0E-23
blast NP_490778 T06A4.2 [Caenorhabditis elegans] gb|AAQ96605.1| MiRP5 [Caenorhabditis elegans] gb|AAC67472.2| Hypothetical protein T06A4.2 [Caenorhabditis elegans] 4.0E-15
blast CAE61322 Hypothetical protein CBG05159 [Caenorhabditis briggsae] 3.0E-9
blast CAE61322 Hypothetical protein CBG05159 [Caenorhabditis briggsae] 5.0E-9
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_490778.1 1 23-45
T06A4.2 1 23-45
SNAP00000004749 1 23-45
GENEFINDER00000004755 1 67-89
Sequence
Target Sequence 
>C. ELEGANS:180129_AT
tctggctcatcatcgacgccatggaccaaagtagtgatattcataattattggcattctc
attgtagccttcatcgtgttcgccgccgcttccattgtgaaatatataaaaagcaagaag
caacaaaagcagccaaagaagaaaacaaaaaagagcacaaaatcgcggagcaagagtttg
aagagcaaacagtctgcagtaaccccaaaaaaaagagcatctccaattccacctcagcca
gctccgccatcatctcccaaaccaactgaagaatctgcgacaccatcctcctcatttgct
ccgtcaactgagccaacaccgacagttccagacaaggttgatgatggctccgaggacacg
gagcacaaagagcaagagcaacagactgttctgtgcacttatggca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTGGCTCATCATCGACGCCATGGA 603 605 49 Antisense
TAATTATTGGCATTCTCATTGTAGC 319 635 92 Antisense
TCATTGTAGCCTTCATCGTGTTCGC 187 619 107 Antisense
CCGCCGCTTCCATTGTGAAATATAT 245 263 131 Antisense
GAGCAAACAGTCTGCAGTAACCCCA 31 391 231 Antisense
CATCATCTCCCAAACCAACTGAAGA 347 213 296 Antisense
AACTGAAGAATCTGCGACACCATCC 217 143 312 Antisense
TTGCTCCGTCAACTGAGCCAACACC 36 701 344 Antisense
CAACACCGACAGTTCCAGACAAGGT 437 189 362 Antisense
AGGTTGATGATGGCTCCGAGGACAC 306 57 383 Antisense
CAGACTGTTCTGTGCACTTATGGCA 685 203 430 Antisense
Sequence Source Affymetrix Proprietary Database