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[180117_s_at]

Description F10G7.8 /REP_DB=WormBase Gene ID /WP=CE02632 /TR=Q19324 /GB=AAA81126.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 89      6grp_high1_10_10 > 98      4grp_highest_8_8 > 02      4grp_high_8_8 > 46      6grp_highest_nonorm > 78      6grp_total > 95      4grp_total > 49      6grp_highest_sub > In      6grp_cgc > 78      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1108
1 2 3 4 range
A 1429.2
(-3)
P 		2365.3
(3)
P 		2288.4
(2.5)
P 		1430.4
(-3)
P 		936
B 1331.3
(-3)
P 		2145.4
(2.5)
P 		2211.6
(3)
P 		1556.5
(-1.5)
P 		880
C 1613.3
(-2.6)
P 		1975.6
(-0.1)
P 		2439.3
(3)
P 		1556
(-3)
P 		883
avr 1457.9
(-3) 		2162.1
(1.9) 		2313.1
(3) 		1514.3
(-2.6) 		855

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1006
1 2 3 4 5 6 range
A 1474.9
(-1.2)
P 		1551.8
(-0.7)
P 		1196.3
(-3)
P 		1729.9
(0.4)
P 		1798.1
(0.9)
P 		2130.6
(3)
P 		934
B 1224.1
(-2.2)
P 		1344.4
(-1.2)
P 		1124.2
(-3)
P 		1572.7
(0.6)
P 		1878.4
(3)
P 		1604.2
(0.8)
P 		754
C 1532.5
(-0.9)
P 		1337.3
(-3)
P 		1832
(2.3)
P 		1803.4
(2)
P 		1886.5
(2.8)
P 		1901.6
(3)
P 		564
avr 1410.5
(-2.7) 		1411.2
(-2.7) 		1384.2
(-3) 		1702
(0.9) 		1854.3
(2.7) 		1878.8
(3) 		495
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 641
1 2 3 4 range
A 520.4
(1.8)
P 		513.4
(1.7)
P 		598.2
(3)
P 		209.4
(-3)
P 		389
B 424.5
(-0.2)
P 		529.9
(3)
P 		512.5
(2.5)
P 		331.7
(-3)
P 		198
C 534
(-0.2)
P 		568
(0.1)
P 		850.2
(3)
P 		262.4
(-3)
P 		588
avr 493
(0.5) 		537.1
(1.2) 		653.6
(3) 		267.8
(-3) 		386

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 503
1 2 3 4 5 6 range
A 271.9
(-2.8)
P 		551.9
(3)
P 		260.5
(-3)
P 		438.7
(0.7)
P 		542.6
(2.8)
P 		518.7
(2.3)
P 		291
B 425.9
(-1.1)
P 		447.5
(-0.9)
P 		273
(-3)
P 		416.6
(-1.2)
P 		763.2
(3)
P 		598.2
(1)
P 		490
C 568
(1.5)
P 		408.1
(-2)
P 		466.3
(-0.7)
P 		362.7
(-3)
P 		610.4
(2.4)
P 		636.6
(3)
P 		274
avr 421.9
(-1.3) 		469.2
(-0.3) 		333.3
(-3) 		406
(-1.6) 		638.7
(3) 		584.5
(1.9) 		305
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180117_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5864
Sequence Type Exemplar sequence
Representative Public ID F10G7.8 NCBI
Target Description F10G7.8 /REP_DB=WormBase Gene ID /WP=CE02632 /TR=Q19324 /GB=AAA81126.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062434(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062434 NCBI Caenorhabditis elegans proteasome Regulatory Particle, Non-ATPase-like family member (rpn-5) (rpn-5) mRNA, complete cds. 11/11 None
SNAP00000005193 ENSEMBL cdna:SNAP chromosome:CEL140:II:4696585:4698279:-1 11/11 None
GENEFINDER00000005207 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4696585:4698279:-1 11/11 None
F10G7.8.1 ENSEMBL cdna:known chromosome:CEL140:II:4696406:4698281:-1 gene:F10G7.8 11/11 None
F10G7.8.2 ENSEMBL cdna:known chromosome:CEL140:II:4696490:4698414:-1 gene:F10G7.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4696583-4698278(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol rpn-5
Ensembl F10G7.8
Entrez Gene 173807 Entrez gene
SwissProt Q19324 EMBL-EBI
WormBase CE02632 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:247257_AT 26S proteasome regulatory subunit, putative (RPN5) at
CANINE:1592527_AT similar to proteasome 26S non-ATPase subunit 12 isoform 2 cfa
CANINE_2:CFAAFFX.17815.1.S1_S_AT similar to proteasome 26S non-ATPase subunit 12 isoform 2 cfa
DROSOPHILA_2:1639091_AT dm
DROSGENOME1:152365_AT dm
CHICKEN:GGAAFFX.2313.2.S1_S_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 gga
HG-U95AV2:1192_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 hs
HC-G110:1192_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 hs
HG-U133A:202353_S_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 hs
HG-U133_PLUS_2:202353_S_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 hs
HG-U133A_2:202353_S_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 hs
HUGENEFL:AB003103_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 hs
U133_X3P:G4506220_3P_A_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 hs
U133_X3P:HS.4295.0.S1_3P_A_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 hs
HG-U133A_2:202352_S_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 hs
HG-FOCUS:202352_S_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 hs
HG-U133_PLUS_2:202352_S_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 hs
HG-U133A:202352_S_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 hs
MU11KSUBA:AA571353_G_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 mm
MU11KSUBA:AA571353_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 mm
MG-U74AV2:93971_F_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 mm
MOE430A:1448492_A_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 mm
MOUSE430A_2:1448492_A_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 mm
MOUSE430_2:1448492_A_AT proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 mm
RG-U34B:RC_AI044322_AT proteasome 26S non-ATPase subunit 12 rn
RAE230B:1392983_AT proteasome 26S non-ATPase subunit 12 rn
RAT230_2:1392983_AT proteasome 26S non-ATPase subunit 12 rn
YEAST_2:1775778_AT Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p Sc
Protein Similarities
Method ID Description E-Value
blast NP_494835 proteasome Regulatory Particle, Non-ATPase-like family member (rpn-5) [Caenorhabditis elegans] gb|AAA81126.1| Proteasome regulatory particle, non-atpase-like protein 5 [Caenorhabditis elegans] 0.0
blast CAE60648 Hypothetical protein CBG04294 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000717 EMBL-EBI Proteasome component region PCI 4.7E-23
Sequence
Target Sequence 
>C. ELEGANS:180117_S_AT
ccattgtctactgtcttttggctccgcacacaaacgagcaatgggatctcctcaaccgaa
ttgccattcaaagagaattggaaaccgttccagactacaagatcattctggatcttttca
tcaaccaagaactcatttctttcaaaggaaccattgtcgcaaagtacgagaagcttttaa
gacgtggaacaactagctcaccagacaccggaattttcgacaaatcaactgaaggagaga
agcggtggtctgatttgcaccttcgtgttggagagcacaacatgcgtatgattgccaagt
actatactcaaatcactttcgaacgtctcgctgagctcctcgacttcccagttgatgaaa
tggaatcatttgtgtgtaacctcattgttaccggtcagatcaccggagcaaaacttcatc
gcccttcccgtattgtcaatcttcgtttgaaaaaggcaaatgttgagcaattggatgtat
gggcgagcaacgtgcac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCATTGTCTACTGTCTTTTGGCTCC 189 271 896 Antisense
AACGAGCAATGGGATCTCCTCAACC 134 145 928 Antisense
TCTCCTCAACCGAATTGCCATTCAA 156 611 942 Antisense
GGAAACCGTTCCAGACTACAAGATC 648 517 975 Antisense
TAGCTCACCAGACACCGGAATTTTC 387 645 1089 Antisense
AGCGGTGGTCTGATTTGCACCTTCG 54 79 1136 Antisense
AAATCACTTTCGAACGTCTCGCTGA 331 117 1205 Antisense
TCCTCGACTTCCCAGTTGATGAAAT 536 593 1232 Antisense
CCTCATTGTTACCGGTCAGATCACC 32 267 1275 Antisense
CCTTCCCGTATTGTCAATCTTCGTT 30 267 1318 Antisense
GGATGTATGGGCGAGCAACGTGCAC 500 513 1368 Antisense
Sequence Source Affymetrix Proprietary Database