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[180116_at]

Description F48C11.3 /REP_DB=WormBase Gene ID /WP=CE10752 /TR=Q93745 /GB=CAB02552.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 03      6grp_high1_10_10 > 10      4grp_highest_8_8 > 61      4grp_high_8_8 > 17      6grp_highest_nonorm > 23      6grp_total > 32      4grp_total > 30      6grp_highest_sub > De      6grp_cgc > 60      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2965
1 2 3 4 range
A 2891.7
(3)
P 		1592.1
(-1.1)
P 		1010.6
(-3)
P 		2649.3
(2.2)
P 		1881
B 3975.9
(3)
P 		1615.6
(-1.9)
P 		1077.6
(-3)
P 		2154.6
(-0.8)
P 		2898
C 3384.3
(3)
P 		1576.1
(-1.6)
P 		1037.8
(-3)
P 		2848.6
(1.6)
P 		2347
avr 3417.3
(3) 		1594.6
(-1.6) 		1042
(-3) 		2550.8
(0.8) 		2375

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1919
1 2 3 4 5 6 range
A 3033.4
(3)
P 		2568.6
(1.5)
P 		3022.2
(3)
P 		2629.1
(1.7)
P 		1847.3
(-0.7)
P 		1114.3
(-3)
P 		1919
B 2753.7
(3)
P 		2722.9
(2.9)
P 		2567.3
(2.2)
P 		2689.4
(2.7)
P 		2307.7
(1)
P 		1428.3
(-3)
P 		1325
C 2587.3
(1.4)
P 		2705.5
(1.9)
P 		2385.5
(0.6)
P 		3002.8
(3)
P 		1760.2
(-1.7)
P 		1431.9
(-3)
P 		1571
avr 2791.5
(3) 		2665.7
(2.5) 		2658.3
(2.5) 		2773.8
(2.9) 		1971.7
(-0.4) 		1324.8
(-3) 		1467
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1018
1 2 3 4 range
A 1052.9
(3)
P 		345.6
(-2.4)
P 		264.2
(-3)
P 		387.8
(-2.1)
P 		789
B 1267.9
(3)
P 		399
(-2.1)
P 		249.7
(-3)
P 		459.2
(-1.8)
P 		1018
C 1120.3
(3)
P 		453.1
(-2.3)
P 		361.7
(-3)
P 		480.3
(-2.1)
P 		759
avr 1147
(3) 		399.2
(-2.2) 		291.9
(-3) 		442.4
(-1.9) 		855

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 688
1 2 3 4 5 6 range
A 559.2
(-0.3)
P 		913.5
(3)
P 		658.2
(0.6)
P 		666.8
(0.7)
P 		557.4
(-0.3)
P 		271.3
(-3)
P 		642
B 958.2
(3)
P 		906.3
(2.3)
P 		623.4
(-1.7)
P 		712.3
(-0.5)
P 		937.6
(2.7)
P 		532.6
(-3)
P 		426
C 959
(3)
P 		825.5
(1.3)
P 		607.2
(-1.4)
P 		603.9
(-1.4)
P 		569.6
(-1.9)
P 		479.4
(-3)
P 		480
avr 825.5
(2.3) 		881.8
(3) 		629.6
(-0.3) 		661
(0.1) 		688.2
(0.4) 		427.8
(-3) 		454
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180116_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17412
Sequence Type Exemplar sequence
Representative Public ID F48C11.3 NCBI
Target Description F48C11.3 /REP_DB=WormBase Gene ID /WP=CE10752 /TR=Q93745 /GB=CAB02552.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077786(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077786 NCBI Caenorhabditis elegans Neuropeptide-Like Protein family member (nlp-3) (nlp-3) mRNA, complete cds. 11/11 None
SNAP00000030512 ENSEMBL cdna:SNAP chromosome:CEL140:X:13177992:13178337:-1 11/11 None
GENEFINDER00000030516 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:13177992:13178337:-1 11/11 None
F48C11.3 ENSEMBL cdna:known chromosome:CEL140:X:13177774:13178374:-1 gene:F48C11.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:13177991-13178337(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nlp-3
Ensembl F48C11.3
Entrez Gene 185978 Entrez gene
SwissProt Q93745 EMBL-EBI
WormBase CE10752 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB02552 Hypothetical protein F48C11.3 [Caenorhabditis elegans] ref|NP_510187.1| Neuropeptide-Like Protein family member (nlp-3) [Caenorhabditis elegans] 2.0E-45
blast CAE61394 Hypothetical protein CBG05250 [Caenorhabditis briggsae] 8.0E-37
blast AAC08343 unknown [Ostertagia ostertagi] 4.0E-14
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_510187.1 1 4-23
F48C11.3 1 4-23
SNAP00000030512 1 4-23
GENEFINDER00000030516 1 4-23
Sequence
Target Sequence 
>C. ELEGANS:180116_AT
aaaatcgtcgcttgcttggtcttactcgctctcagcgttatgtgtgtctactctgctccc
tatgaatttagagcaaaaagagcaatcaatccattcttggattctatgggaaaacgagct
gtcaacccgttccttgactcaattggcaaacgatctttccgtccagacatgatcactgag
gagaagagatattttgatagcttggccggtcaatctttgggaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAATCGTCGCTTGCTTGGTCTTAC 173 131 19 Antisense
TCGCTCTCAGCGTTATGTGTGTCTA 708 599 44 Antisense
AGCGTTATGTGTGTCTACTCTGCTC 68 83 52 Antisense
TCTACTCTGCTCCCTATGAATTTAG 431 613 65 Antisense
GAGCAATCAATCCATTCTTGGATTC 276 391 98 Antisense
TGGGAAAACGAGCTGTCAACCCGTT 589 551 125 Antisense
ACCCGTTCCTTGACTCAATTGGCAA 262 91 143 Antisense
ATTGGCAAACGATCTTTCCGTCCAG 153 3 160 Antisense
TTCCGTCCAGACATGATCACTGAGG 465 697 175 Antisense
GAGATATTTTGATAGCTTGGCCGGT 412 397 204 Antisense
GCTTGGCCGGTCAATCTTTGGGAAA 443 305 218 Antisense
Sequence Source Affymetrix Proprietary Database