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[180106_at]

Description F29A7.4 /REP_DB=WormBase Gene ID /WP=CE09775 /TR=O16206 /GB=AAB65274.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 24      4grp_total > 47      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 171
1 2 3 4 range
A 148.1
(3)
A 		13.9
(-3)
A 		13.5
(-3)
A 		24.4
(-2.5)
A 		135
B 180.1
(3)
P 		15.3
(-2.9)
A 		11.5
(-3)
A 		11.1
(-3)
A 		169
C 182.5
(3)
A 		65.8
(-1.3)
A 		30.4
(-2.6)
A 		18.2
(-3)
A 		164
avr 170.2
(3) 		31.7
(-2.5) 		18.5
(-3) 		17.9
(-3) 		152

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 100
1 2 3 4 5 6 range
A 99.1
(3)
A 		95.6
(2.8)
A 		41.6
(-0.9)
A 		78
(1.6)
A 		9.7
(-3)
A 		14.8
(-2.7)
A 		89
B 106.4
(3)
P 		74.6
(1)
A 		97.4
(2.4)
A 		12
(-3)
A 		45.6
(-0.9)
A 		25.2
(-2.2)
A 		94
C 108
(3)
P 		58.1
(-0)
A 		54.1
(-0.2)
A 		60.3
(0.1)
A 		21.4
(-2.2)
A 		8.4
(-3)
A 		100
avr 104.5
(3) 		76.1
(1.1) 		64.4
(0.3) 		50.1
(-0.7) 		25.6
(-2.4) 		16.1
(-3) 		88
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 58
1 2 3 4 range
A 53.9
(3)
A 		3
(-3)
A 		3.5
(-2.9)
A 		3.6
(-2.9)
A 		51
B 57.4
(3)
P 		3.8
(-2.8)
A 		2.7
(-3)
A 		2.4
(-3)
A 		55
C 60.4
(3)
A 		18.9
(-1.3)
A 		10.6
(-2.2)
A 		3.1
(-3)
A 		57
avr 57.2
(3) 		8.6
(-2.4) 		5.6
(-2.7) 		3
(-3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 37
1 2 3 4 5 6 range
A 18.3
(-0)
A 		34
(3)
A 		9.1
(-1.8)
A 		19.8
(0.3)
A 		2.9
(-3)
A 		3.6
(-2.9)
A 		31
B 37
(3)
P 		24.8
(0.8)
A 		23.6
(0.6)
A 		3.2
(-3)
A 		18.5
(-0.3)
A 		9.4
(-1.9)
A 		34
C 40
(3)
P 		17.7
(-0.6)
A 		13.8
(-1.2)
A 		12.1
(-1.5)
A 		6.9
(-2.3)
A 		2.8
(-3)
A 		37
avr 31.8
(3) 		25.5
(1.6) 		15.5
(-0.7) 		11.7
(-1.6) 		9.4
(-2.1) 		5.3
(-3) 		27
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180106_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5048
Sequence Type Exemplar sequence
Representative Public ID F29A7.4 NCBI
Target Description F29A7.4 /REP_DB=WormBase Gene ID /WP=CE09775 /TR=O16206 /GB=AAB65274.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061947(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061947 NCBI Caenorhabditis elegans F29A7.4 (F29A7.4) mRNA, complete cds. 10/11 None
SNAP00000030191 ENSEMBL cdna:SNAP chromosome:CEL140:II:2748823:2751573:1 10/11 None
GENEFINDER00000030201 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2749898:2751573:1 10/11 None
F29A7.4 ENSEMBL cdna:known chromosome:CEL140:II:2748588:2751573:1 gene:F29A7.4 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2748775-2751572(+) 84.26 100.0
Public Domain and Genome References
Gene Title
Ensembl F29A7.4
Entrez Gene 185104 Entrez gene
SwissProt O16206 EMBL-EBI
WormBase CE36835 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB65274 Hypothetical protein F29A7.4 [Caenorhabditis elegans] ref|NP_494348.2| F29A7.4 [Caenorhabditis elegans] 1.0E-139
blast AAB65274 Hypothetical protein F29A7.4 [Caenorhabditis elegans] ref|NP_494348.2| F29A7.4 [Caenorhabditis elegans] 6.0E-88
blast AAB65274 Hypothetical protein F29A7.4 [Caenorhabditis elegans] ref|NP_494348.2| F29A7.4 [Caenorhabditis elegans] 5.0E-80
blast CAE72411 Hypothetical protein CBG19572 [Caenorhabditis briggsae] 5.0E-78
blast CAE72411 Hypothetical protein CBG19572 [Caenorhabditis briggsae] 1.0E-77
blast CAE72411 Hypothetical protein CBG19572 [Caenorhabditis briggsae] 1.0E-72
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494348.2 1 13-35
F29A7.4 1 13-35
Sequence
Target Sequence 
>C. ELEGANS:180106_AT
tggacggacaccgattcgacggagccctgcggaccctcaaatggagtgccgatcagacgt
actctgtgaaagtgaccacaaaaccggcaaccgaagtgacccgaatcaacgtgaacggca
acgatttggaggtgacaaaggagaagatcggcgttttttccggcaattggaacactgcag
gagcagaaatttcgaagcgaggaacccgacatccgctcatttttctggtgaattttgctc
cggagggacagatgaaggccgagtttcagtcgaaaatttattcgaagcttttggccaagt
ggctcagtgtgattttcgccccggctaccaatttcaagtccgggtcatttacagtgagga
ctgaggattttacttttcaaaaaaatttgcccgagtggctcagtgcgaatttcgccccgg
ctaccaatttcacgtccgggaacgactcccacgcagtttggggtcacaagatgaatagcg
tcgagttcaagtgcagcggcgacgacgatggatac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGACGGACACCGATTCGACGGAGC 602 523 275 Antisense
CCCTGCGGACCCTCAAATGGAGTGC 141 259 299 Antisense
GGAGTGCCGATCAGACGTACTCTGT 237 517 317 Antisense
GGCAACCGAAGTGACCCGAATCAAC 294 531 360 Antisense
GAAGATCGGCGTTTTTTCCGGCAAT 515 335 417 Antisense
TCGAAGCGAGGAACCCGACATCCGC 311 601 466 Antisense
ACATCCGCTCATTTTTCTGGTGAAT 42 107 483 Antisense
GAGTGGCTCAGTGCGAATTTCGCCC 141 401 667 Antisense
GACTCCCACGCAGTTTGGGGTCACA 388 371 718 Antisense
AGCGTCGAGTTCAAGTGCAGCGGCG 399 79 751 Antisense
GTGCAGCGGCGACGACGATGGATAC 214 481 765 Antisense
Sequence Source Affymetrix Proprietary Database