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[180103_at]

Description C32E8.1 /REP_DB=WormBase Gene ID /WP=CE08525 /TR=P91131 /GB=AAB42321.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 75      4grp_total > 19      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 63
1 2 3 4 range
A 8.5
(3)
A 		5.2
(0.2)
A 		1.5
(-3)
A 		6.8
(1.5)
A 		7
B 9.5
(0.1)
A 		2.8
(-3)
A 		12.2
(1.3)
A 		15.8
(3)
A 		13
C 3.4
(-3)
A 		40.3
(0.6)
A 		3.5
(-3)
A 		64.2
(3)
P 		61
avr 7.1
(-2.6) 		16.1
(-0.3) 		5.7
(-3) 		28.9
(3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 36
1 2 3 4 5 6 range
A 6.8
(-2.3)
A 		3.3
(-3)
A 		10.6
(-1.6)
A 		33.9
(3)
A 		3.9
(-2.9)
A 		33.5
(2.9)
A 		31
B 18.7
(1.2)
A 		0.9
(-3)
A 		4.7
(-2.1)
A 		1.8
(-2.8)
A 		6.6
(-1.7)
A 		26.4
(3)
A 		26
C 2.2
(-3)
A 		7.6
(-2.1)
A 		36.6
(3)
P 		34.3
(2.6)
A 		2.9
(-2.9)
A 		2.7
(-2.9)
A 		34
avr 9.2
(-1.4) 		3.9
(-3) 		17.3
(1.1) 		23.3
(3) 		4.5
(-2.8) 		20.9
(2.3) 		19
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 3.1
(3)
A 		1.1
(-1.4)
A 		0.4
(-3)
A 		1
(-1.7)
A 		3
B 3
(2.1)
A 		0.7
(-3)
A 		2.8
(1.7)
A 		3.4
(3)
A 		3
C 1.1
(-3)
A 		11.6
(3)
A 		1.2
(-2.9)
A 		10.8
(2.5)
P 		11
avr 2.4
(-1.5) 		4.5
(2) 		1.5
(-3) 		5.1
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 1.2
(-3)
A 		1.2
(-3)
A 		2.3
(-2.1)
A 		8.6
(3)
A 		1.2
(-3)
A 		8.2
(2.7)
A 		7
B 6.5
(0.9)
A 		0.3
(-3)
A 		1.1
(-2.5)
A 		0.5
(-2.9)
A 		2.7
(-1.5)
A 		9.8
(3)
A 		10
C 0.8
(-3)
A 		2.3
(-1.9)
A 		9.3
(3)
P 		6.9
(1.3)
A 		0.9
(-2.9)
A 		0.9
(-2.9)
A 		9
avr 2.8
(-1.2) 		1.3
(-3) 		4.2
(0.5) 		5.3
(1.8) 		1.6
(-2.6) 		6.3
(3) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180103_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.60
Sequence Type Exemplar sequence
Representative Public ID C32E8.1 NCBI
Target Description C32E8.1 /REP_DB=WormBase Gene ID /WP=CE08525 /TR=P91131 /GB=AAB42321.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058822(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058822 NCBI Caenorhabditis elegans C32E8.1 (C32E8.1) mRNA, complete cds. 11/11 None
SNAP00000040655 ENSEMBL cdna:SNAP chromosome:CEL140:I:3796168:3796686:1 11/11 None
GENEFINDER00000040666 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3796168:3796686:1 11/11 None
C32E8.1 ENSEMBL cdna:known chromosome:CEL140:I:3796168:3796686:1 gene:C32E8.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3796167-3796686(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C32E8.1
Entrez Gene 183123 Entrez gene
SwissProt P91131 EMBL-EBI
WormBase CE08525 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB42321 Hypothetical protein C32E8.1 [Caenorhabditis elegans] ref|NP_491223.1| C32E8.1 [Caenorhabditis elegans] 2.0E-86
blast CAE67264 Hypothetical protein CBG12711 [Caenorhabditis briggsae] 3.0E-15
blast CAB04460 Hypothetical protein F53F8.3 [Caenorhabditis elegans] ref|NP_507996.1| F53F8.3 [Caenorhabditis elegans] 4.0E-14
Protein Domains
Database ID Description E-Value
Pfam IPR001841 EMBL-EBI Zn-finger, RING 6.3E-6
Sequence
Target Sequence 
>C. ELEGANS:180103_AT
gtaggacacgatgcggtgccagtgaaaaaagttccttgggaagagcaacagttgatgaag
aaagtgaagcgtctgcaatcggagaacgaaaagttgaaggtgcagctggatgatatggtg
gattgttttgtggaaacgaaggaggctcttgagaaggaaaaacgtgtgacggacttcctg
aaaaaggcgatttccgatctcaaaactcttccgattgaatgtgagatttgtgcagtacaa
tatggggattctggaatgaccgtgcctcgtgttctgccgtgtggccacacaatttgcacc
gaatgcgctggaaatattatccaaaacaagatgatccgttgtccattcgataga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTAGGACACGATGCGGTGCCAGTGA 129 455 55 Antisense
AGTTCCTTGGGAAGAGCAACAGTTG 214 63 84 Antisense
AAGTGAAGCGTCTGCAATCGGAGAA 274 157 116 Antisense
GGTGCAGCTGGATGATATGGTGGAT 604 499 153 Antisense
GTGGAAACGAAGGAGGCTCTTGAGA 145 491 184 Antisense
GAAAAACGTGTGACGGACTTCCTGA 161 355 211 Antisense
GCGATTTCCGATCTCAAAACTCTTC 424 289 241 Antisense
AAAACTCTTCCGATTGAATGTGAGA 167 131 256 Antisense
TGGGGATTCTGGAATGACCGTGCCT 251 553 297 Antisense
ATTTGCACCGAATGCGCTGGAAATA 659 17 346 Antisense
GATGATCCGTTGTCCATTCGATAGA 94 411 384 Antisense
Sequence Source Affymetrix Proprietary Database