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[180096_at]

Description T12B5.4 /REP_DB=WormBase Gene ID /WP=CE20062 /TR=Q9TZ23 /GB=AAC68930.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 30      4grp_total > 12      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 263
1 2 3 4 range
A 147.4
(-0.1)
A 		72.2
(-2.6)
A 		60.5
(-3)
A 		238.2
(3)
A 		178
B 94.7
(-0.9)
A 		112.8
(-0.1)
A 		44.4
(-3)
A 		188.3
(3)
A 		144
C 132.3
(-1.7)
P 		83.2
(-3)
A 		98.9
(-2.6)
A 		307.7
(3)
P 		225
avr 124.8
(-1.1) 		89.4
(-2.3) 		67.9
(-3) 		244.7
(3) 		177

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 167
1 2 3 4 5 6 range
A 64.3
(-2.3)
A 		115.6
(0.2)
A 		153.4
(2.1)
P 		171.4
(3)
P 		51.3
(-2.9)
A 		50.1
(-3)
A 		121
B 80.1
(-1.8)
A 		150.8
(0.7)
A 		214
(3)
P 		117.4
(-0.5)
A 		103.9
(-1)
A 		46.9
(-3)
A 		167
C 81.4
(-2.9)
A 		170.4
(1.1)
A 		145.5
(-0)
A 		213.1
(3)
P 		78.3
(-3)
A 		81.9
(-2.8)
A 		135
avr 75.3
(-2.2) 		145.6
(1.6) 		171
(3) 		167.3
(2.8) 		77.8
(-2) 		59.6
(-3) 		111
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 43
1 2 3 4 range
A 53.7
(3)
A 		15.7
(-3)
A 		15.8
(-3)
A 		34.9
(0)
A 		38
B 30.2
(1)
A 		27.8
(0.5)
A 		10.3
(-3)
A 		40.1
(3)
A 		30
C 43.8
(1.3)
P 		23.9
(-3)
A 		34.5
(-0.7)
A 		51.9
(3)
P 		28
avr 42.6
(3) 		22.5
(-2.4) 		20.2
(-3) 		42.3
(2.9) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 11.9
(-3)
A 		41.1
(2.5)
A 		33.4
(1.1)
P 		43.5
(3)
P 		15.5
(-2.3)
A 		12.2
(-2.9)
A 		32
B 27.9
(-1.2)
A 		50.2
(2.7)
A 		52
(3)
P 		31.1
(-0.6)
A 		42.2
(1.3)
A 		17.5
(-3)
A 		35
C 30.2
(-1.9)
A 		52
(3)
A 		37
(-0.4)
A 		42.9
(1)
P 		25.3
(-3)
A 		27.4
(-2.5)
A 		27
avr 23.3
(-2.1) 		47.8
(3) 		40.8
(1.5) 		39.2
(1.2) 		27.7
(-1.2) 		19
(-3) 		29
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180096_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8109
Sequence Type Exemplar sequence
Representative Public ID T12B5.4 NCBI
Target Description T12B5.4 /REP_DB=WormBase Gene ID /WP=CE20062 /TR=Q9TZ23 /GB=AAC68930.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064902(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064902 NCBI Caenorhabditis elegans T12B5.4 (T12B5.4) mRNA, complete cds. 11/11 None
SNAP00000007702 ENSEMBL cdna:SNAP chromosome:CEL140:III:950869:952042:1 11/11 None
GENEFINDER00000007709 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:950869:952605:1 11/11 None
T12B5.4 ENSEMBL cdna:known chromosome:CEL140:III:950869:952042:1 gene:T12B5.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:950868-952042(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T12B5.4
Entrez Gene 188443 Entrez gene
SwissProt Q9TZ23 EMBL-EBI
WormBase CE20062 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC68930 Hypothetical protein T12B5.4 [Caenorhabditis elegans] ref|NP_497303.1| T12B5.4 [Caenorhabditis elegans] 1.0E-159
blast CAB03494 Hypothetical protein ZC47.6 [Caenorhabditis elegans] ref|NP_497363.1| ZC47.6 [Caenorhabditis elegans] 1.0E-56
blast AAC68929 Hypothetical protein T12B5.3 [Caenorhabditis elegans] ref|NP_497304.1| T12B5.3 [Caenorhabditis elegans] 1.0E-54
Protein Domains
Database ID Description E-Value
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 3.5E-52
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 1.4E-12
Sequence
Target Sequence 
>C. ELEGANS:180096_AT
atggaccaaacctcacaattgtgcttcagaaacaaaagaaaagagcttctcaacagcata
acctccgttacagacattttgaaaaataaagaaaatctctgcgtgaaggaaatattactt
aacacattttcatttgatgatgccttgcttattcttccgtgtttcaatgctcaagtcctg
gaaagtatcgagttatgggaagctgctccgattgaccattgtgaacggctcaccggcttg
gaccagtggaaaaatgcgaaacgtttccgaactcacgattctcggttccagaatgaaaaa
ttgcaaattggacagttgtttcattttgaaaagtttcaagtcgaatttgataatttctca
attctagacgttacgaaagtcaaagaggatctcttgaaaagaagcgaattcaaagaatgc
tctctcataattggtaggttcgattcaacgccgttcaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGACCAAACCTCACAATTGTGCT 452 51 319 Antisense
AGAGCTTCTCAACAGCATAACCTCC 59 71 360 Antisense
ATAACCTCCGTTACAGACATTTTGA 104 25 376 Antisense
TGATGATGCCTTGCTTATTCTTCCG 577 565 453 Antisense
CGTGTTTCAATGCTCAAGTCCTGGA 665 243 476 Antisense
GCTGCTCCGATTGACCATTGTGAAC 401 297 520 Antisense
TGACCATTGTGAACGGCTCACCGGC 135 571 531 Antisense
CTCACCGGCTTGGACCAGTGGAAAA 653 227 547 Antisense
ATGCGAAACGTTTCCGAACTCACGA 493 41 572 Antisense
CGAACTCACGATTCTCGGTTCCAGA 427 251 586 Antisense
TAGGTTCGATTCAACGCCGTTCAAA 177 651 753 Antisense
Sequence Source Affymetrix Proprietary Database