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[180091_at]

Description C32E8.3 /REP_DB=WormBase Gene ID /WP=CE08527 /TR=P91127 /GB=AAB42320.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 05      6grp_high1_10_10 > 50      4grp_highest_8_8 > 25      4grp_high_8_8 > 59      6grp_highest_nonorm > 45      6grp_total > 51      4grp_total > 60      6grp_highest_sub > 03_04      6grp_high1_sub > 03_04      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 922
1 2 3 4 range
A 1435.4
(3)
P 		1301.7
(1.9)
P 		1274.2
(1.7)
P 		696.1
(-3)
P 		739
B 1474.8
(1.8)
P 		1432.7
(1.5)
P 		1618.3
(3)
P 		886.2
(-3)
P 		732
C 1384.6
(1.6)
P 		1467.1
(2.1)
P 		1595.9
(3)
P 		697.3
(-3)
P 		899
avr 1431.6
(2.5) 		1400.5
(2.2) 		1496.1
(3) 		759.9
(-3) 		736

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1466
1 2 3 4 5 6 range
A 2058.8
(-0.1)
P 		2284.6
(1)
P 		2691.5
(3)
P 		2565.6
(2.4)
P 		1834
(-1.2)
P 		1479
(-3)
P 		1213
B 2699.4
(1.6)
P 		2375.3
(-0.3)
P 		2945.2
(3)
P 		2633.1
(1.2)
P 		2240.3
(-1)
P 		1900.5
(-3)
P 		1045
C 2256.8
(-0.2)
P 		2335
(0.6)
P 		2325.6
(0.5)
P 		2572.8
(3)
P 		1988.1
(-3)
P 		2006
(-2.8)
P 		585
avr 2338.3
(0.8) 		2331.6
(0.7) 		2654.1
(3) 		2590.5
(2.6) 		2020.8
(-1.4) 		1795.2
(-3) 		859
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 454
1 2 3 4 range
A 522.6
(3)
P 		282.6
(-0.4)
P 		333.1
(0.3)
P 		101.9
(-3)
P 		421
B 470.3
(3)
P 		353.8
(0.5)
P 		375
(1)
P 		188.9
(-3)
P 		281
C 458.3
(1.7)
P 		421.8
(1.2)
P 		556.2
(3)
P 		117.6
(-3)
P 		439
avr 483.7
(3) 		352.7
(0.7) 		421.4
(1.9) 		136.1
(-3) 		348

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 579
1 2 3 4 5 6 range
A 379.5
(-2.7)
P 		812.5
(3)
P 		586.2
(-0)
P 		650.7
(0.9)
P 		553.4
(-0.4)
P 		360.1
(-3)
P 		452
B 939.3
(3)
P 		790.6
(-0.7)
P 		715.2
(-2.6)
P 		697.4
(-3)
P 		910.2
(2.3)
P 		708.7
(-2.7)
P 		242
C 836.5
(3)
P 		712.5
(0.7)
P 		591.9
(-1.6)
P 		517.4
(-3)
P 		643.3
(-0.6)
P 		671.5
(-0.1)
P 		319
avr 718.4
(1.3) 		771.9
(3) 		631.1
(-1.4) 		621.8
(-1.7) 		702.3
(0.8) 		580.1
(-3) 		192
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180091_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.198
Sequence Type Exemplar sequence
Representative Public ID C32E8.3 NCBI
Target Description C32E8.3 /REP_DB=WormBase Gene ID /WP=CE08527 /TR=P91127 /GB=AAB42320.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058818(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058818 NCBI Caenorhabditis elegans C32E8.3 (C32E8.3) mRNA, complete cds. 11/11 None
SNAP00000040651 ENSEMBL cdna:SNAP chromosome:CEL140:I:3786596:3787383:1 11/11 None
GENEFINDER00000040660 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3786596:3787383:1 11/11 None
C32E8.3 ENSEMBL cdna:known chromosome:CEL140:I:3786596:3787477:1 gene:C32E8.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3786595-3787383(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C32E8.3
Entrez Gene 171948 Entrez gene
SwissProt P91127 EMBL-EBI
WormBase CE08527 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1591684_AT similar to chromosome 14 open reading frame 8 cfa
CANINE:1590520_AT similar to chromosome 14 open reading frame 8 cfa
CANINE:1590412_AT similar to chromosome 14 open reading frame 8 cfa
CANINE_2:CFA.6708.1.A1_AT similar to chromosome 14 open reading frame 8 cfa
CANINE_2:CFA.7340.1.S1_S_AT similar to chromosome 14 open reading frame 8 cfa
CANINE_2:CFA.8120.1.A1_AT similar to chromosome 14 open reading frame 8 cfa
CHICKEN:GGA.16625.1.S1_AT similar to RIKEN cDNA 2700055K07 gga
HU35KSUBD:RC_AA448281_AT chromosome 14 open reading frame 8 hs
U133_X3P:HS.99168.0.A1_3P_S_AT chromosome 14 open reading frame 8 hs
HG-U133_PLUS_2:231140_AT chromosome 14 open reading frame 8 hs
HG-U133B:231140_AT chromosome 14 open reading frame 8 hs
HG-U95B:52882_AT chromosome 14 open reading frame 8 hs
U133_X3P:HS2.406966.1.S1_3P_AT Chromosome 14 open reading frame 8 hs
HG-U133_PLUS_2:1559785_AT Chromosome 14 open reading frame 8 hs
MG-U74BV2:110607_AT gene model 77, (NCBI) mm
Protein Similarities
Method ID Description E-Value
blast AAB42320 Hypothetical protein C32E8.3 [Caenorhabditis elegans] ref|NP_491219.1| C32E8.3 [Caenorhabditis elegans] sp|P91127|YBYK_CAEEL Hypothetical protein C32E8.3 in chromosome I 5.0E-94
blast CAE66664 Hypothetical protein CBG12002 [Caenorhabditis briggsae] 5.0E-86
blast 1PUL Chain , Solution Structure For The 21kda Caenorhabditis Elegans Protein Ce32e8.3. Northeast Structural Genomics Consortium Target Wr33 3.0E-59
Protein Domains
Database ID Description E-Value
Pfam IPR008907 EMBL-EBI P25-alpha 1.1E-110
Sequence
Target Sequence 
>C. ELEGANS:180091_AT
gctgctgctggattcaactgggatgatgctgatgtgaagaaaagatgggacgcgttcacc
aaattcggagctgctaccgccaccgaaatgactggaaagaacttcgacaagtggcttaag
gatgctggtgtcctcgacaacaaagccatcaccgggacaatgaccggaatcgctttcagc
aaagttactggacctaaaaagaaggccactttcgacgaaacgaagaaagtgctggcattt
gtcgccgaggatcgcgcgagacaaagtaagaagccgattcaggacgaactggacgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTGCTGCTGGATTCAACTGGGATG 337 297 19 Antisense
GATGGGACGCGTTCACCAAATTCGG 269 409 62 Antisense
CACCAAATTCGGAGCTGCTACCGCC 449 201 75 Antisense
TGCTACCGCCACCGAAATGACTGGA 191 555 90 Antisense
GGCTTAAGGATGCTGGTGTCCTCGA 509 523 131 Antisense
GTGTCCTCGACAACAAAGCCATCAC 557 485 146 Antisense
CAAAGCCATCACCGGGACAATGACC 279 191 159 Antisense
GACAATGACCGGAATCGCTTTCAGC 163 365 174 Antisense
GAAAGTGCTGGCATTTGTCGCCGAG 694 359 243 Antisense
TGTCGCCGAGGATCGCGCGAGACAA 622 557 258 Antisense
GCCGATTCAGGACGAACTGGACGCA 450 281 291 Antisense
Sequence Source Affymetrix Proprietary Database