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[180089_at]

Description C50F2.8 /REP_DB=WormBase Gene ID /WP=CE08914 /TR=P91182 /GB=AAB37801.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 41      6grp_high1_10_10 > 03      4grp_high_8_8 > 42      6grp_highest_nonorm > 80      6grp_total > 24      4grp_total > 45      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 314
1 2 3 4 range
A 377.4
(3)
P 		188.6
(-1.3)
P 		188.5
(-1.3)
P 		111.5
(-3)
P 		266
B 375.9
(3)
P 		337.3
(2)
P 		234.1
(-0.8)
P 		150.8
(-3)
P 		225
C 425.7
(3)
P 		342.4
(1.2)
P 		191.6
(-2)
P 		145.6
(-3)
A 		280
avr 393
(3) 		289.4
(0.6) 		204.7
(-1.4) 		136
(-3) 		257

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 279
1 2 3 4 5 6 range
A 278.8
(1.4)
P 		333.4
(3)
P 		185.1
(-1.4)
P 		209.7
(-0.7)
P 		130.6
(-3)
P 		139.7
(-2.7)
P 		203
B 385.3
(3)
P 		274.8
(0.5)
P 		216.2
(-0.8)
P 		199.9
(-1.2)
P 		121.4
(-3)
P 		145
(-2.5)
P 		264
C 400
(3)
P 		300.8
(0.8)
P 		274.1
(0.2)
P 		178.8
(-1.9)
P 		129.5
(-3)
P 		167.4
(-2.2)
P 		271
avr 354.7
(3) 		303
(1.6) 		225.1
(-0.4) 		196.1
(-1.2) 		127.2
(-3) 		150.7
(-2.4) 		228
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 125
1 2 3 4 range
A 137.4
(3)
P 		40.9
(-1.8)
P 		49.3
(-1.4)
P 		16.3
(-3)
P 		121
B 119.9
(3)
P 		83.3
(0.5)
P 		54.2
(-1.5)
P 		32.1
(-3)
P 		88
C 140.9
(3)
P 		98.4
(0.8)
P 		66.8
(-0.8)
P 		24.5
(-3)
A 		116
avr 132.7
(3) 		74.2
(-0.2) 		56.8
(-1.2) 		24.3
(-3) 		108

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 114
1 2 3 4 5 6 range
A 51.4
(-1.8)
P 		118.6
(3)
P 		40.3
(-2.6)
P 		53.2
(-1.6)
P 		39.4
(-2.6)
P 		34
(-3)
P 		85
B 134.1
(3)
P 		91.5
(-0)
P 		52.5
(-2.8)
P 		52.9
(-2.7)
P 		49.3
(-3)
P 		54.1
(-2.7)
P 		85
C 148.3
(3)
P 		91.8
(-0)
P 		69.8
(-1.2)
P 		35.9
(-3)
P 		41.9
(-2.7)
P 		56.1
(-1.9)
P 		112
avr 111.3
(3) 		100.6
(2.1) 		54.2
(-2.1) 		47.3
(-2.7) 		43.5
(-3) 		48.1
(-2.6) 		68
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180089_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.168
Sequence Type Exemplar sequence
Representative Public ID C50F2.8 NCBI
Target Description C50F2.8 /REP_DB=WormBase Gene ID /WP=CE08914 /TR=P91182 /GB=AAB37801.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058859(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058859 NCBI Caenorhabditis elegans C50F2.8 (C50F2.8) mRNA, complete cds. 11/11 None
C50F2.8 ENSEMBL cdna:known chromosome:CEL140:I:3901434:3905202:-1 gene:C50F2.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3901555-3905202(-) 93.56 100.0
Public Domain and Genome References
Gene Title
Ensembl C50F2.8
Entrez Gene 183676 Entrez gene
SwissProt P91182 EMBL-EBI
WormBase CE34276 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37801 Hypothetical protein C50F2.8 [Caenorhabditis elegans] ref|NP_491260.2| C50F2.8 [Caenorhabditis elegans] 0.0
blast CAE66695 Hypothetical protein CBG12035 [Caenorhabditis briggsae] 0.0
blast CAG11988 unnamed protein product [Tetraodon nigroviridis] 2.0E-40
Protein Domains
Database ID Description E-Value
Pfam IPR008144 EMBL-EBI Guanylate kinase 5.0E-18
Sequence
Target Sequence 
>C. ELEGANS:180089_AT
agtcgatgcttttgaggctggctccacattatttttcaacaattgttccattaacctcac
gagcccaacgacctggagaagtggagggacgtgaatatcaatttgtccgaaaagaagaaa
tttaccaaaagattcgtgaaggtggaatggttgagtggggggaattggatagtcaactct
atggaacatcggcagactccgttagaaatgaaatccgaagtggacgaatgtgtgtattgg
atgctgctcctcagagtgtaaattatttatataacgctgaattcatgccattcgtggttc
atgtcgtgcccccaccaattgaagagtttacacaacttgaggcgggaaaattttatttga
tttttgcaaagtttatcgagaaatttaaattttttttcaaattatggcgggaaagtcaac
attttattgtcaatttccgtcggactcaaggccaactccaatcaatttgtgacgaaagtg
caaagataggattggaatgtaaatcaaagattcatctaacacttgttaataggaatattg
atgttacattcaaacgattggttgactcgttggaaactcttcgctacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTCGATGCTTTTGAGGCTGGCTCC 307 59 911 Antisense
GAGGCTGGCTCCACATTATTTTTCA 706 405 924 Antisense
CAATTGTTCCATTAACCTCACGAGC 624 179 950 Antisense
ACGAGCCCAACGACCTGGAGAAGTG 330 93 969 Antisense
GGAACATCGGCAGACTCCGTTAGAA 196 529 1093 Antisense
GTATTGGATGCTGCTCCTCAGAGTG 216 453 1144 Antisense
ATAACGCTGAATTCATGCCATTCGT 520 25 1181 Antisense
CATGCCATTCGTGGTTCATGTCGTG 691 209 1194 Antisense
GTGCCCCCACCAATTGAAGAGTTTA 233 477 1216 Antisense
GACTCAAGGCCAACTCCAATCAATT 5 375 1353 Antisense
GACTCGTTGGAAACTCTTCGCTACG 591 373 1474 Antisense
Sequence Source Affymetrix Proprietary Database