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[180081_s_at]

Description W05F2.7 /REP_DB=WormBase Gene ID /WP=CE14624 /TR=Q9TYU0 /GB=AAC78217.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 78      6grp_high1_10_10 > 87      4grp_highest_8_8 > 05      4grp_high_8_8 > 30      6grp_highest_nonorm > 93      6grp_total > 87      4grp_total > 33      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 184
1 2 3 4 range
A 237.7
(-3)
P 		377.7
(2.1)
P 		373.7
(1.9)
P 		403.7
(3)
P 		166
B 220.1
(-3)
P 		302.6
(-0.2)
P 		305.4
(-0.1)
P 		394.7
(3)
P 		175
C 250.7
(-3)
P 		381.8
(3)
P 		296.3
(-0.9)
P 		321.1
(0.2)
P 		131
avr 236.2
(-3) 		354
(2.2) 		325.1
(0.9) 		373.2
(3) 		137

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 543
1 2 3 4 5 6 range
A 195.5
(-3)
P 		217.8
(-2.7)
P 		207.5
(-2.8)
P 		313
(-1.4)
P 		386.7
(-0.4)
P 		633.8
(3)
P 		438
B 208.9
(-2.6)
P 		173.1
(-3)
P 		233.4
(-2.3)
P 		360.1
(-0.7)
P 		420.8
(0.1)
P 		658.9
(3)
P 		486
C 215.1
(-2.4)
P 		162.2
(-3)
P 		210.2
(-2.5)
P 		222.6
(-2.3)
P 		396.9
(-0.4)
P 		705.1
(3)
P 		543
avr 206.5
(-2.7) 		184.4
(-3) 		217
(-2.6) 		298.6
(-1.6) 		401.5
(-0.3) 		665.9
(3) 		482
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 56
1 2 3 4 range
A 86.5
(1.3)
P 		82
(0.6)
P 		97.7
(3)
P 		59.1
(-3)
P 		39
B 70.2
(-3)
P 		74.7
(-1.1)
P 		70.8
(-2.7)
P 		84.1
(3)
P 		14
C 83
(0.1)
P 		109.8
(3)
P 		103.3
(2.3)
P 		54.1
(-3)
P 		56
avr 79.9
(0.4) 		88.8
(2.6) 		90.6
(3) 		65.8
(-3) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 210
1 2 3 4 5 6 range
A 36
(-3)
P 		77.5
(-0.9)
P 		45.2
(-2.5)
P 		79.4
(-0.8)
P 		116.7
(1.1)
P 		154.3
(3)
P 		118
B 72.7
(-2.5)
P 		57.6
(-3)
P 		56.7
(-3)
P 		95.4
(-1.8)
P 		171
(0.6)
P 		245.7
(3)
P 		189
C 79.7
(-1.9)
P 		49.5
(-2.9)
P 		53.5
(-2.7)
P 		44.8
(-3)
P 		128.4
(-0.4)
P 		236
(3)
P 		191
avr 62.8
(-2.6) 		61.5
(-2.6) 		51.8
(-3) 		73.2
(-2.2) 		138.7
(0.3) 		212
(3) 		160
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180081_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.113
Sequence Type Exemplar sequence
Representative Public ID W05F2.7 NCBI
Target Description W05F2.7 /REP_DB=WormBase Gene ID /WP=CE14624 /TR=Q9TYU0 /GB=AAC78217.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058746(9), NM_066902(11), NM_066903(11), NM_001027482(11), NM_001027483(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058746 NCBI Caenorhabditis elegans W05F2.7 (W05F2.7) mRNA, complete cds. 9/11 A 
NM_066902 NCBI Caenorhabditis elegans H04D03.2b (H04D03.2) mRNA, complete cds. 11/11 A 
NM_066903 NCBI Caenorhabditis elegans H04D03.2a (H04D03.2) mRNA, complete cds. 11/11 A 
NM_001027482 NCBI Caenorhabditis elegans HP1 Like (heterochromatin protein) family member (hpl-2) (hpl-2) mRNA, complete cds. 11/11 None
NM_001027483 NCBI Caenorhabditis elegans HP1 Like (heterochromatin protein) family member (hpl-2) (hpl-2) mRNA, complete cds. 11/11 None
SNAP00000029372 ENSEMBL cdna:SNAP chromosome:CEL140:I:3362370:3363363:1 11/11 None
GENEFINDER00000029378 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3362370:3363363:1 11/11 None
SNAP00000026564 ENSEMBL cdna:SNAP chromosome:CEL140:III:10438073:10447423:-1 11/11 None
GENEFINDER00000026568 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:10438073:10438282:-1 11/11 None
SNAP00000018661 ENSEMBL cdna:SNAP chromosome:CEL140:III:4782473:4782697:1 11/11 None
GENEFINDER00000018677 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4782488:4782697:1 11/11 None
SNAP00000033491 ENSEMBL cdna:SNAP chromosome:CEL140:III:10752201:10755387:-1 11/11 None
GENEFINDER00000033495 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:10752201:10753387:-1 11/11 None
W05F2.7 ENSEMBL cdna:known chromosome:CEL140:I:3351446:3363363:1 gene:W05F2.7 9/11 A 
H04D03.2b ENSEMBL cdna:known chromosome:CEL140:III:10438073:10442287:-1 gene:H04D03.2 11/11 A 
H04D03.2a ENSEMBL cdna:known chromosome:CEL140:III:10438073:10442287:-1 gene:H04D03.2 11/11 A 
K01G5.2b ENSEMBL cdna:known chromosome:CEL140:III:10752140:10755392:-1 gene:K01G5.2 11/11 A 
K01G5.2c ENSEMBL cdna:known chromosome:CEL140:III:10752197:10755387:-1 gene:K01G5.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176506 3 2 NM_001027482 171755_x_at (9), 171757_x_at (10), 172917_x_at (10), 172918_x_at (11), 180081_s_at (11)
NM_001027483 171755_x_at (10), 171757_x_at (9), 172917_x_at (11), 172918_x_at (10), 180081_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3351445-3363363(+) 98.49 100.0
Public Domain and Genome References
Gene Title
Ensembl W05F2.7
H04D03.2
K01G5.2
Entrez Gene 189218 Entrez gene
SwissProt Q9TYU0 EMBL-EBI
WormBase CE32227 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_491147 W05F2.7 [Caenorhabditis elegans] gb|AAC78217.2| Hypothetical protein W05F2.7 [Caenorhabditis elegans] 0.0
blast CAB54258 Hypothetical protein H04D03.2b [Caenorhabditis elegans] ref|NP_499303.1| H04D03.2b [Caenorhabditis elegans] 0.0
blast CAA15957 Hypothetical protein H04D03.2a [Caenorhabditis elegans] ref|NP_499304.1| H04D03.2a [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR008251 EMBL-EBI Chromo shadow 2.1E-32
Pfam IPR000953 EMBL-EBI Chromo 2.6E-14
Sequence
Target Sequence 
>C. ELEGANS:180081_S_AT
ggccaatggagatctgctttccagcttcccacaggatttccttgaagactctggcgatgg
agaagttcacgaggcggccatcctctccgttttcgatgacaattcgaatagcccgccacc
gtgtccgatcgttgtcgacactgtaccagacgatgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCCAATGGAGATCTGCTTTCCAGC 258 545 3930 Antisense
AATGGAGATCTGCTTTCCAGCTTCC 340 163 3934 Antisense
GATTTCCTTGAAGACTCTGGCGATG 601 427 3964 Antisense
GACTCTGGCGATGGAGAAGTTCACG 597 373 3976 Antisense
TGGAGAAGTTCACGAGGCGGCCATC 532 549 3987 Antisense
CATCCTCTCCGTTTTCGATGACAAT 489 211 4008 Antisense
TCCTCTCCGTTTTCGATGACAATTC 521 593 4010 Antisense
CACCGTGTCCGATCGTTGTCGACAC 677 199 4046 Antisense
TCCGATCGTTGTCGACACTGTACCA 502 595 4053 Antisense
GATCGTTGTCGACACTGTACCAGAC 196 417 4056 Antisense
TGTCGACACTGTACCAGACGATGAT 658 557 4062 Antisense
Sequence Source Affymetrix Proprietary Database