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[180077_at]

Description C32E8.6 /REP_DB=WormBase Gene ID /WP=CE27814 /TR=P91123 /GB=AAB42324.2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 48      4grp_total > 39      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 81
1 2 3 4 range
A 26.5
(1.8)
A 		29.5
(3)
A 		14
(-3)
A 		26.8
(2)
A 		16
B 61.3
(3)
A 		14.3
(-2.7)
A 		33.7
(-0.3)
A 		11.6
(-3)
A 		50
C 92.3
(3)
A 		14.5
(-3)
A 		15.3
(-2.9)
A 		64.3
(0.8)
A 		78
avr 60
(3) 		19.4
(-3) 		21
(-2.8) 		34.2
(-0.8) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 68.7
(3)
A 		44.8
(0.7)
A 		5.1
(-3)
A 		20.9
(-1.5)
A 		9.3
(-2.6)
A 		8.3
(-2.7)
A 		64
B 5.1
(-3)
A 		20.2
(-0.8)
A 		45.9
(3)
A 		10.7
(-2.2)
A 		8.6
(-2.5)
A 		44.9
(2.9)
A 		41
C 38.3
(-0.6)
A 		19.8
(-2.6)
A 		16.6
(-3)
A 		31
(-1.4)
A 		65.1
(2.5)
A 		69.8
(3)
A 		53
avr 37.4
(1.9) 		28.3
(-0.8) 		22.5
(-2.5) 		20.9
(-3) 		27.7
(-1) 		41
(3) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 28
1 2 3 4 range
A 9.7
(3)
A 		6.4
(-0.3)
A 		3.7
(-3)
A 		3.9
(-2.8)
A 		6
B 19.5
(3)
A 		3.5
(-2.6)
A 		7.8
(-1.1)
A 		2.5
(-3)
A 		17
C 30.6
(3)
A 		4.2
(-3)
A 		5.3
(-2.8)
A 		10.8
(-1.5)
A 		26
avr 19.9
(3) 		4.7
(-3) 		5.6
(-2.6) 		5.7
(-2.6) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 12.7
(1.7)
A 		15.9
(3)
A 		1.1
(-3)
A 		5.3
(-1.3)
A 		2.8
(-2.3)
A 		2
(-2.6)
A 		15
B 1.8
(-3)
A 		6.7
(-1)
A 		11.2
(0.8)
A 		2.8
(-2.6)
A 		3.5
(-2.3)
A 		16.7
(3)
A 		15
C 14.2
(0.1)
A 		6
(-2.4)
A 		4.2
(-3)
A 		6.2
(-2.4)
A 		21.1
(2.3)
A 		23.4
(3)
A 		19
avr 9.6
(0.1) 		9.5
(0.1) 		5.5
(-2.5) 		4.8
(-3) 		9.1
(-0.2) 		14
(3) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180077_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.96
Sequence Type Exemplar sequence
Representative Public ID C32E8.6 NCBI
Target Description C32E8.6 /REP_DB=WormBase Gene ID /WP=CE27814 /TR=P91123 /GB=AAB42324.2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_170845(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_170845 NCBI Caenorhabditis elegans C32E8.6a (C32E8.6) mRNA, complete cds. 11/11 None
SNAP00000040647 ENSEMBL cdna:SNAP chromosome:CEL140:I:3773112:3776646:1 11/11 None
GENEFINDER00000040669 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3773132:3777297:1 11/11 None
C32E8.6a ENSEMBL cdna:known chromosome:CEL140:I:3774713:3777470:1 gene:C32E8.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_170846 6/11 Cross Hyb Matching Probes None
C32E8.6b 6/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
171945 2 1 NM_170845 180077_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3774716-3776646(+) 100.0 100.0
Public Domain and Genome References
Ensembl C32E8.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB42324 Hypothetical protein C32E8.6a [Caenorhabditis elegans] ref|NP_740831.1| C32E8.6a [Caenorhabditis elegans] 0.0
blast CAE66661 Hypothetical protein CBG11998 [Caenorhabditis briggsae] 0.0
blast CAE66661 Hypothetical protein CBG11998 [Caenorhabditis briggsae] 1.0E-179
blast AAM15557 Hypothetical protein C32E8.6b [Caenorhabditis elegans] ref|NP_740832.1| C32E8.6b [Caenorhabditis elegans] 1.0E-169
blast CAE66661 Hypothetical protein CBG11998 [Caenorhabditis briggsae] 1.0E-158
Protein Families
Method ID Description E-Value
ec 4CL1_ARATH (Q42524) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1) 4.0E-90
ec 4CL1_ARATH (Q42524) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1) 1.0E-104
ec 4CL1_ARATH (Q42524) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1) 1.0E-109
Protein Domains
Database ID Description E-Value
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 1.2E-44
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 3.6E-40
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 4.0E-13
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 7.6E-43
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 3.6E-40
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 4.0E-13
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 3.6E-40
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 4.0E-13
Sequence
Target Sequence 
>C. ELEGANS:180077_AT
gtctcagtgttgtttctggttccacccgttctcaatttcttcgctaatcatccattagtg
tccagttacgacctttccgcattgaaaaccatatatgttggcgctgctgcaagtcctccg
gagaattttgcaaaggttgcggaaagattaccggagctcgagaatttgatacaattgtac
ggaacatctgaatgtggtgtactcctgtgcagtactggaaaaggaaaagcttctggaaag
actgttggatggccattccctttcgtcgagcttaagatcaatcctgaaaataatgagatt
tttgtgaaatctgccacagctatggaagaaggattcatggagactggcgacctgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCTCAGTGTTGTTTCTGGTTCCAC 567 469 727 Antisense
GTTCTCAATTTCTTCGCTAATCATC 68 443 754 Antisense
TTAGTGTCCAGTTACGACCTTTCCG 624 677 781 Antisense
ATATATGTTGGCGCTGCTGCAAGTC 78 19 817 Antisense
GCTGCAAGTCCTCCGGAGAATTTTG 23 295 832 Antisense
GAAAGATTACCGGAGCTCGAGAATT 483 359 868 Antisense
TGAATGTGGTGTACTCCTGTGCAGT 391 575 915 Antisense
AAGACTGTTGGATGGCCATTCCCTT 679 153 964 Antisense
CATTCCCTTTCGTCGAGCTTAAGAT 325 217 980 Antisense
GTGAAATCTGCCACAGCTATGGAAG 704 481 1030 Antisense
GGATTCATGGAGACTGGCGACCTGG 569 509 1057 Antisense
Sequence Source Affymetrix Proprietary Database