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[180052_at]

Description C10B5.3 /REP_DB=WormBase Gene ID /WP=CE08054 /TR=O16319 /GB=AAC24249.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 30      6grp_high1_10_10 > 13      4grp_highest_8_8 > 40      4grp_high_8_8 > 43      6grp_highest_nonorm > 51      6grp_total > 12      4grp_total > 44      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 476
1 2 3 4 range
A 582.3
(3)
P 		405.7
(-1.3)
P 		430.3
(-0.7)
P 		334.7
(-3)
P 		248
B 570
(0.5)
P 		768.3
(3)
P 		444.7
(-1.1)
P 		292
(-3)
P 		476
C 520.3
(3)
P 		512.8
(2.8)
P 		347.1
(-2.3)
P 		323
(-3)
P 		197
avr 557.5
(2.9) 		562.3
(3) 		407.4
(-0.8) 		316.6
(-3) 		246

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 522
1 2 3 4 5 6 range
A 607.8
(0.5)
P 		827.1
(3)
P 		643.4
(0.9)
P 		465.8
(-1.1)
P 		468.7
(-1.1)
P 		304.7
(-3)
P 		522
B 795.8
(3)
P 		704.3
(1.7)
P 		711.4
(1.8)
P 		525.2
(-0.7)
P 		587.9
(0.1)
P 		361.4
(-3)
P 		434
C 759.5
(2.2)
P 		804.4
(3)
P 		498.4
(-2.1)
P 		578.4
(-0.8)
P 		560.3
(-1.1)
P 		446.6
(-3)
P 		358
avr 721
(2.2) 		778.6
(3) 		617.7
(0.6) 		523.1
(-0.8) 		539
(-0.5) 		370.9
(-3) 		408
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 163
1 2 3 4 range
A 212
(3)
P 		88.1
(-1.6)
P 		112.5
(-0.7)
P 		49
(-3)
P 		163
B 181.8
(2.6)
P 		189.8
(3)
P 		103
(-1.1)
P 		62.2
(-3)
P 		128
C 172.2
(3)
P 		147.4
(1.7)
P 		121
(0.4)
P 		54.5
(-3)
P 		118
avr 188.7
(3) 		141.8
(0.9) 		112.2
(-0.4) 		55.2
(-3) 		134

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 220
1 2 3 4 5 6 range
A 112
(-2)
P 		294.2
(3)
P 		140.1
(-1.2)
P 		118.1
(-1.8)
P 		141.4
(-1.2)
P 		74.2
(-3)
P 		220
B 276.9
(3)
P 		234.4
(1.2)
P 		172.7
(-1.4)
P 		139.1
(-2.8)
P 		238.8
(1.4)
P 		134.8
(-3)
P 		142
C 281.5
(3)
P 		245.5
(1.7)
P 		126.9
(-2.6)
P 		116.3
(-3)
P 		181.3
(-0.6)
P 		149.5
(-1.8)
P 		165
avr 223.5
(1.5) 		258
(3) 		146.6
(-1.8) 		124.5
(-2.8) 		187.2
(-0.1) 		119.5
(-3) 		139
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180052_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11820
Sequence Type Exemplar sequence
Representative Public ID C10B5.3 NCBI
Target Description C10B5.3 /REP_DB=WormBase Gene ID /WP=CE08054 /TR=O16319 /GB=AAC24249.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072813(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072813 NCBI Caenorhabditis elegans C10B5.3 (C10B5.3) mRNA, complete cds. 10/11 None
SNAP00000001563 ENSEMBL cdna:SNAP chromosome:CEL140:V:8583331:8587114:-1 9/11 None
GENEFINDER00000001565 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8586438:8590807:-1 10/11 None
C10B5.3 ENSEMBL cdna:known chromosome:CEL140:V:8586438:8587114:-1 gene:C10B5.3 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8586438-8587115(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C10B5.3
Entrez Gene 182491 Entrez gene
SwissProt O16319 EMBL-EBI
WormBase CE35228 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC24249 Hypothetical protein C10B5.3 [Caenorhabditis elegans] ref|NP_505214.2| C10B5.3 [Caenorhabditis elegans] 1.0E-52
blast AAC24249 Hypothetical protein C10B5.3 [Caenorhabditis elegans] ref|NP_505214.2| C10B5.3 [Caenorhabditis elegans] 4.0E-52
blast AAC24249 Hypothetical protein C10B5.3 [Caenorhabditis elegans] ref|NP_505214.2| C10B5.3 [Caenorhabditis elegans] 3.0E-49
blast CAE58361 Hypothetical protein CBG01486 [Caenorhabditis briggsae] 4.0E-46
blast CAE58361 Hypothetical protein CBG01486 [Caenorhabditis briggsae] 1.0E-45
blast CAE58361 Hypothetical protein CBG01486 [Caenorhabditis briggsae] 2.0E-43
Sequence
Target Sequence 
>C. ELEGANS:180052_AT
gacgcgtcggtgctcaaaagtgtgctcgaacagtttgtcgatgagatcggagaggaatcg
gttgatctgatcgaagatcgaccacgttttgcacatgctgtcgttgttgtcacggatgat
gctacaggtgaactgtgtcaagcatcttgcatcagacaacttgatgatgtcattctacat
gagacaagaatgactggagtactgtatttcatggacaaggccgtcatgggattactaaca
tctgaatggatggatgaggaaattcaacgtgctctccgtgagctcactgagtcacgtgaa
cttctagacactccgcgtaatagtgtctttgtagaagagaaacatgagaagcagaagact
gtaacaaccactacaaccagtacagagaccagtcgtaacaccagtagccgagttgagttt
ccatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACGCGTCGGTGCTCAAAAGTGTGC 566 379 22 Antisense
GAAGATCGACCACGTTTTGCACATG 145 339 94 Antisense
TTGCACATGCTGTCGTTGTTGTCAC 128 703 110 Antisense
GGTGAACTGTGTCAAGCATCTTGCA 658 499 148 Antisense
GACAAGGCCGTCATGGGATTACTAA 106 359 235 Antisense
GAGGAAATTCAACGTGCTCTCCGTG 486 403 277 Antisense
TGCTCTCCGTGAGCTCACTGAGTCA 128 581 291 Antisense
CACGTGAACTTCTAGACACTCCGCG 561 199 314 Antisense
CTCCGCGTAATAGTGTCTTTGTAGA 591 229 332 Antisense
AGACCAGTCGTAACACCAGTAGCCG 128 69 407 Antisense
CCAGTAGCCGAGTTGAGTTTCCATC 599 269 422 Antisense
Sequence Source Affymetrix Proprietary Database