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[180044_at]

Description T20H9.4 /REP_DB=WormBase Gene ID /WP=CE13862 /TR=O44890 /GB=AAB95053.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 28      4grp_total > 14      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 30
(2.2)
A 		1
(-3)
A 		3.4
(-2.6)
A 		34.5
(3)
A 		34
B 36.1
(3)
A 		21.6
(0.4)
A 		2.8
(-2.9)
A 		2
(-3)
A 		34
C 10.2
(-2.6)
A 		7.1
(-3)
A 		13.9
(-2.1)
A 		51.1
(3)
A 		44
avr 25.4
(2) 		9.9
(-2.1) 		6.7
(-3) 		29.2
(3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 28.9
(1.2)
A 		33.6
(3)
A 		25.6
(-0)
A 		19.4
(-2.4)
A 		32.3
(2.5)
A 		17.7
(-3)
A 		16
B 19.3
(3)
A 		3.8
(-2.3)
A 		15.1
(1.6)
A 		1.6
(-3)
A 		17.2
(2.3)
A 		11.1
(0.2)
A 		18
C 26.9
(3)
A 		12.8
(-0.3)
A 		1.6
(-2.9)
A 		14.2
(0)
A 		1.1
(-3)
A 		14.5
(0.1)
A 		26
avr 25
(3) 		16.7
(-0.7) 		14.1
(-1.9) 		11.7
(-3) 		16.9
(-0.7) 		14.4
(-1.8) 		13
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 10.9
(3)
A 		0.2
(-3)
A 		0.9
(-2.6)
A 		5.1
(-0.3)
A 		11
B 11.5
(3)
A 		5.3
(-0.4)
A 		0.7
(-2.8)
A 		0.4
(-3)
A 		11
C 3.4
(-1.7)
A 		2
(-3)
A 		4.8
(-0.5)
A 		8.6
(3)
A 		7
avr 8.6
(3) 		2.5
(-2.6) 		2.1
(-3) 		4.7
(-0.6) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12
1 2 3 4 5 6 range
A 5.3
(-2.2)
A 		11.9
(3)
A 		5.6
(-2)
A 		4.9
(-2.5)
A 		9.7
(1.3)
A 		4.3
(-3)
A 		8
B 6.7
(2.7)
A 		1.3
(-2.2)
A 		3.7
(0)
A 		0.4
(-3)
A 		7
(3)
A 		4.2
(0.5)
A 		7
C 10
(3)
A 		3.9
(-0.8)
A 		0.4
(-2.9)
A 		2.9
(-1.4)
A 		0.3
(-3)
A 		4.8
(-0.2)
A 		10
avr 7.3
(3) 		5.7
(0.9) 		3.2
(-2.3) 		2.7
(-3) 		5.7
(0.9) 		4.4
(-0.8) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180044_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8126
Sequence Type Exemplar sequence
Representative Public ID T20H9.4 NCBI
Target Description T20H9.4 /REP_DB=WormBase Gene ID /WP=CE13862 /TR=O44890 /GB=AAB95053.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065124(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065124 NCBI Caenorhabditis elegans T20H9.4 (T20H9.4) mRNA, complete cds. 11/11 None
SNAP00000029857 ENSEMBL cdna:SNAP chromosome:CEL140:III:2253912:2254770:1 10/11 None
GENEFINDER00000029859 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2253912:2256936:1 10/11 None
T20H9.4 ENSEMBL cdna:known chromosome:CEL140:III:2253437:2254770:1 gene:T20H9.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2253436-2254770(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T20H9.4
Entrez Gene 188667 Entrez gene
SwissProt O44890 EMBL-EBI
WormBase CE13862 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB95052 Hypothetical protein T20H9.3 [Caenorhabditis elegans] ref|NP_497526.1| T20H9.3 [Caenorhabditis elegans] 1.0E-157
blast AAB95053 Hypothetical protein T20H9.4 [Caenorhabditis elegans] ref|NP_497525.1| T20H9.4 [Caenorhabditis elegans] 1.0E-140
blast AAB95053 Hypothetical protein T20H9.4 [Caenorhabditis elegans] ref|NP_497525.1| T20H9.4 [Caenorhabditis elegans] 1.0E-132
blast AAB95053 Hypothetical protein T20H9.4 [Caenorhabditis elegans] ref|NP_497525.1| T20H9.4 [Caenorhabditis elegans] 1.0E-131
blast NP_503905 T28A11.21 [Caenorhabditis elegans] gb|AAC48129.2| Hypothetical protein T28A11.21 [Caenorhabditis elegans] 4.0E-48
blast CAB03489 Hypothetical protein ZC47.4 [Caenorhabditis elegans] ref|NP_497367.1| ZC47.4 [Caenorhabditis elegans] 8.0E-39
blast AAC68938 Hypothetical protein T12B5.11 [Caenorhabditis elegans] ref|NP_497302.1| T12B5.11 [Caenorhabditis elegans] 9.0E-35
blast CAB03489 Hypothetical protein ZC47.4 [Caenorhabditis elegans] ref|NP_497367.1| ZC47.4 [Caenorhabditis elegans] 5.0E-33
Protein Domains
Database ID Description E-Value
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 1.8E-37
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 2.3E-49
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 8.9E-58
Sequence
Target Sequence 
>C. ELEGANS:180044_AT
atggtgggacaactgtgcatcataatggacaaaaaaagagaatcgaaggagaacaatata
aagaaagatcgatcaaggatttggcagttatctttaagcatgcttctatcctagagctct
acaatttgggagataatgtaaaactactcgttgatattttgaaagactcatttattcacg
tggaatcaattaaactcaattgtatcaccatcgataagtgtcttgcaattctttctcgct
gcaagaccaaagtgctcaagaatattgatctgttcatagtttaccgatctgaacaattcg
aacggattactcatttggagcaatggaagaattcaagaaccttaaagtgctgggcagatc
atgtgatcagcgatccgttgccaatcgaacagttctttcattttgagcagtttgaaattg
aagaacatggtatttcaattgagaaagtgattagtattagagatgtaagttccatcgatc
cagacgaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGTGGGACAACTGTGCATCATAA 306 51 173 Antisense
GGCAGTTATCTTTAAGCATGCTTCT 222 537 255 Antisense
AGCATGCTTCTATCCTAGAGCTCTA 616 77 269 Antisense
TAAACTCAATTGTATCACCATCGAT 145 641 363 Antisense
TAAGTGTCTTGCAATTCTTTCTCGC 399 637 387 Antisense
AATTCTTTCTCGCTGCAAGACCAAA 648 175 399 Antisense
ATCTGTTCATAGTTTACCGATCTGA 143 35 440 Antisense
AATTCGAACGGATTACTCATTTGGA 583 175 467 Antisense
TGATCAGCGATCCGTTGCCAATCGA 343 565 536 Antisense
ATCGAACAGTTCTTTCATTTTGAGC 3 37 556 Antisense
GTAAGTTCCATCGATCCAGACGAAT 189 461 637 Antisense
Sequence Source Affymetrix Proprietary Database