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[180043_at]

Description F54C4.4 /REP_DB=WormBase Gene ID /WP=CE19893 /TR=Q9TZ91 /GB=AAC68777.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 26      6grp_high1_10_10 > 52      6grp_highest_nonorm > 90      6grp_total > 62      4grp_total > 10      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 147
1 2 3 4 range
A 97
(-1.7)
P 		107.4
(-1.2)
A 		69.4
(-3)
A 		197.2
(3)
P 		128
B 86.2
(-2)
A 		61
(-3)
A 		108.1
(-1.1)
P 		207.8
(3)
P 		147
C 83.4
(-3)
P 		127
(-0.7)
M 		103.3
(-1.9)
P 		195.9
(3)
P 		113
avr 88.9
(-3) 		98.5
(-2.5) 		93.6
(-2.7) 		200.3
(3) 		111

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 221
1 2 3 4 5 6 range
A 127.2
(-1.9)
P 		149.7
(-1.1)
P 		263.1
(3)
P 		120.5
(-2.1)
P 		147.6
(-1.1)
P 		95.8
(-3)
P 		167
B 134.6
(-3)
P 		230.6
(0.2)
P 		316.3
(3)
P 		204.5
(-0.7)
P 		211.9
(-0.4)
P 		168.4
(-1.9)
P 		182
C 111.3
(-2.4)
P 		174.8
(0.2)
P 		98.2
(-3)
P 		240
(3)
P 		161.5
(-0.3)
P 		203.8
(1.5)
P 		142
avr 124.4
(-3) 		185
(0.6) 		225.9
(3) 		188.3
(0.8) 		173.7
(-0.1) 		156
(-1.1) 		102
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29
1 2 3 4 range
A 35.3
(3)
P 		23.3
(-1.2)
A 		18.2
(-3)
A 		28.9
(0.8)
P 		17
B 27.5
(-0.5)
A 		15.1
(-3)
A 		25
(-1)
P 		44.3
(3)
P 		29
C 27.6
(-3)
P 		36.5
(3)
M 		36
(2.7)
P 		33
(0.6)
P 		9
avr 30.1
(-0.1) 		25
(-3) 		26.4
(-2.2) 		35.4
(3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 63
1 2 3 4 5 6 range
A 23.4
(-3)
P 		53.3
(2.3)
P 		57.3
(3)
P 		30.6
(-1.7)
P 		44.6
(0.8)
P 		23.3
(-3)
P 		34
B 46.8
(-3)
P 		76.7
(1.6)
P 		76.8
(1.6)
P 		54.2
(-1.9)
P 		86.1
(3)
P 		62.8
(-0.6)
P 		39
C 41.3
(-0.7)
P 		53.3
(0.9)
P 		25
(-3)
P 		48.3
(0.2)
P 		52.2
(0.8)
P 		68.2
(3)
P 		43
avr 37.2
(-3) 		61.1
(3) 		53
(1) 		44.4
(-1.2) 		61
(3) 		51.4
(0.6) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180043_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8107
Sequence Type Exemplar sequence
Representative Public ID F54C4.4 NCBI
Target Description F54C4.4 /REP_DB=WormBase Gene ID /WP=CE19893 /TR=Q9TZ91 /GB=AAC68777.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064736(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064736 NCBI Caenorhabditis elegans F54C4.4 (F54C4.4) mRNA, complete cds. 11/11 None
GENEFINDER00000020636 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:86779:87258:-1 11/11 None
F54C4.4 ENSEMBL cdna:known chromosome:CEL140:III:86779:87258:-1 gene:F54C4.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:86778-87258(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F54C4.4
Entrez Gene 186221 Entrez gene
SwissProt Q9TZ91 EMBL-EBI
WormBase CE19893 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC68777 Hypothetical protein F54C4.4 [Caenorhabditis elegans] ref|NP_497137.1| F54C4.4 [Caenorhabditis elegans] 3.0E-50
Sequence
Target Sequence 
>C. ELEGANS:180043_AT
cgaggagaccatgatggctgctgagcagagttcttcagggatttctgccaattcaaatag
caactgtagtgcatcgaaaaccgaaaaagcgtcgaaaaaaccgaaaaacgccattccaaa
agtcaaaaactctggaattcacaagaaacccgccaaatcggactttgacgagtttctgag
cagcatgaagagccgattatctcaagctaccagtatgaaaagcaataaagagcaactcaa
gatgttagaggagacgcagaagcttattttcgatagaatcacaaagtcagccgatgcgcg
ggatatgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGAGGAGACCATGATGGCTGCTGAG 478 249 33 Antisense
GGCTGCTGAGCAGAGTTCTTCAGGG 708 539 48 Antisense
GTTCTTCAGGGATTTCTGCCAATTC 422 443 62 Antisense
CAACTGTAGTGCATCGAAAACCGAA 594 145 93 Antisense
GGAATTCACAAGAAACCCGCCAAAT 489 533 166 Antisense
ACCCGCCAAATCGGACTTTGACGAG 22 91 180 Antisense
CTTTGACGAGTTTCTGAGCAGCATG 531 219 195 Antisense
GAGCAGCATGAAGAGCCGATTATCT 106 389 210 Antisense
GAGCCGATTATCTCAAGCTACCAGT 162 385 222 Antisense
GGAGACGCAGAAGCTTATTTTCGAT 159 519 282 Antisense
AAGTCAGCCGATGCGCGGGATATGG 296 157 316 Antisense
Sequence Source Affymetrix Proprietary Database