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[180039_at]

Description Y54E5A.1 /REP_DB=WormBase Gene ID /WP=CE20312 /TR=Q9XWK1 /GB=CAA21659.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 10      6grp_high1_10_10 > 14      4grp_highest_8_8 > 30      4grp_high_8_8 > 05      6grp_highest_nonorm > 72      6grp_total > 03      4grp_total > 03      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 439
1 2 3 4 range
A 418.5
(-2)
P 		353.5
(-3)
P 		382.3
(-2.6)
P 		757.1
(3)
P 		404
B 416.3
(-1.5)
P 		318.5
(-3)
P 		351.8
(-2.5)
P 		699.4
(3)
P 		381
C 369
(-2.5)
P 		338.6
(-3)
P 		393.8
(-2.1)
P 		722.5
(3)
P 		384
avr 401.3
(-2) 		336.9
(-3) 		376
(-2.4) 		726.3
(3) 		389

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 194
1 2 3 4 5 6 range
A 490.1
(3)
P 		457.2
(0.6)
P 		406.9
(-3)
P 		416.3
(-2.3)
P 		450.2
(0.1)
P 		409.2
(-2.8)
P 		83
B 479.2
(3)
P 		479.9
(3)
P 		358.8
(-2.3)
P 		414.6
(0.2)
P 		437.5
(1.2)
P 		342.1
(-3)
P 		138
C 428
(-0.9)
P 		435.4
(-0.7)
P 		474.8
(0.8)
P 		536.4
(3)
P 		370.4
(-3)
P 		395.5
(-2.1)
P 		166
avr 465.8
(3) 		457.5
(2.4) 		413.5
(-0.8) 		455.8
(2.3) 		419.4
(-0.3) 		382.3
(-3) 		84
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 152.4
(3)
P 		76.7
(-3)
P 		99.9
(-1.2)
P 		110.8
(-0.3)
P 		76
B 132.7
(1.6)
P 		78.7
(-3)
P 		81.5
(-2.8)
P 		149
(3)
P 		70
C 122.1
(0.7)
P 		97.4
(-3)
P 		137.3
(3)
P 		121.8
(0.7)
P 		40
avr 135.7
(3) 		84.3
(-3) 		106.2
(-0.4) 		127.2
(2) 		51

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 91
1 2 3 4 5 6 range
A 90.4
(-2.9)
P 		162.6
(3)
P 		88.6
(-3)
P 		105.6
(-1.6)
P 		135.9
(0.8)
P 		99.6
(-2.1)
P 		74
B 166.8
(2.3)
P 		159.7
(1.8)
P 		87.1
(-3)
P 		109.8
(-1.5)
P 		177.8
(3)
P 		127.6
(-0.3)
P 		91
C 158.6
(3)
P 		132.9
(-0)
P 		120.8
(-1.5)
P 		107.9
(-3)
P 		119.9
(-1.6)
P 		132.4
(-0.1)
P 		51
avr 138.6
(1.5) 		151.7
(3) 		98.8
(-3) 		107.8
(-2) 		144.5
(2.2) 		119.9
(-0.6) 		53
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180039_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.816
Sequence Type Exemplar sequence
Representative Public ID Y54E5A.1 NCBI
Target Description Y54E5A.1 /REP_DB=WormBase Gene ID /WP=CE20312 /TR=Q9XWK1 /GB=CAA21659.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061148(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061148 NCBI Caenorhabditis elegans Y54E5A.1 (Y54E5A.1) mRNA, complete cds. 11/11 A 
Y54E5A.1 ENSEMBL cdna:known chromosome:CEL140:I:14696791:14699832:-1 gene:Y54E5A.1 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000041557 8/11 Cross Hyb Matching Probes None
SNAP00000041546 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14697878-14699763(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y54E5A.1
Entrez Gene 173327 Entrez gene
SwissProt Q9XWK1 EMBL-EBI
WormBase CE28791 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.27381.1.S1_AT similar to sphingolipid C4-hydroxylase/delta 4-desaturase cfa
CHICKEN:GGA.10747.1.S1_S_AT degenerative spermatocyte homolog 2, lipid desaturase (Drosophila) gga
CHICKEN:GGAAFFX.23495.1.S1_AT degenerative spermatocyte homolog 2, lipid desaturase (Drosophila) gga
U133_X3P:HS.159643.0.A1_3P_AT degenerative spermatocyte homolog 2, lipid desaturase (Drosophila) hs
HG-U133_PLUS_2:236496_AT degenerative spermatocyte homolog 2, lipid desaturase (Drosophila) hs
HG-U133B:236496_AT degenerative spermatocyte homolog 2, lipid desaturase (Drosophila) hs
HG-U95E:83944_AT degenerative spermatocyte homolog 2, lipid desaturase (Drosophila) hs
MOE430A:1424549_AT degenerative spermatocyte homolog 2 (Drosophila), lipid desaturase mm
MOUSE430A_2:1424549_AT degenerative spermatocyte homolog 2 (Drosophila), lipid desaturase mm
MOUSE430_2:1424549_AT degenerative spermatocyte homolog 2 (Drosophila), lipid desaturase mm
MU11KSUBA:AA683962_S_AT degenerative spermatocyte homolog 2 (Drosophila), lipid desaturase mm
MG-U74AV2:101384_AT degenerative spermatocyte homolog 2 (Drosophila), lipid desaturase mm
MU19KSUBB:TC31871_AT Degenerative spermatocyte homolog 2 (Drosophila), lipid desaturase (Degs2), mRNA mm
RG-U34A:RC_AI639085_AT similar to RIKEN cDNA 2210008A03 gene (predicted) rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAC70116 Hypothetical protein Y54E5A.1 [Caenorhabditis elegans] emb|CAA21659.2| Hypothetical protein Y54E5A.1 [Caenorhabditis elegans] ref|NP_493549.1| Y54E5A.1 [Caenorhabditis elegans] 0.0
blast CAE64141 Hypothetical protein CBG08757 [Caenorhabditis briggsae] 0.0
blast EAL34272 GA21524-PA [Drosophila pseudoobscura] 1.0E-104
Protein Domains
Database ID Description E-Value
Pfam IPR005804 EMBL-EBI Fatty acid desaturase 4.6E-50
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493549.1 3 66-88,160-177,198-220
Y54E5A.1 3 66-88,160-177,198-220
Sequence
Target Sequence 
>C. ELEGANS:180039_AT
aatttgccgattgctgtgccgatatcagtgtcttttaagaagtatcatgtggagcatcat
cgatatttgggagaagatggcctagatacagatgtccctacaacatttgaggccgagttc
ttcaccaccgcgccgcggaagcttctgtggcttgccttacaaccgtttttctatggtttc
aggccattgattatttataagaaagctcctactgatatggaaattttaaatgctattatt
cagatctcattcgacttattaatactgcacttttttggcgttaaatcactattctatcta
ctttttggaacaattatcagtatgggactccatccaagtgctgggcactttatctcggag
cactatgcttttaaggaggatcaggagacttttagttactatggtctctggaacctatgt
acctttaacgtaggctaccacgtcgaacaccacgacttcccatacatccccggacgagat
ctcccgaaattacgtgcaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATTTGCCGATTGCTGTGCCGATAT 311 175 349 Antisense
AGATGGCCTAGATACAGATGTCCCT 646 67 423 Antisense
AACATTTGAGGCCGAGTTCTTCACC 284 137 450 Antisense
CTTCTGTGGCTTGCCTTACAACCGT 144 219 490 Antisense
TAATACTGCACTTTTTTGGCGTTAA 545 633 608 Antisense
ATCACTATTCTATCTACTTTTTGGA 124 27 633 Antisense
AGTATGGGACTCCATCCAAGTGCTG 689 61 667 Antisense
GTGCTGGGCACTTTATCTCGGAGCA 32 479 686 Antisense
TGGTCTCTGGAACCTATGTACCTTT 597 547 750 Antisense
TAACGTAGGCTACCACGTCGAACAC 604 637 774 Antisense
GAGATCTCCCGAAATTACGTGCAAT 608 395 824 Antisense
Sequence Source Affymetrix Proprietary Database