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[180034_at]

Description T20H9.1 /REP_DB=WormBase Gene ID /WP=CE13856 /TR=O44892 /GB=AAB95050.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 09      4grp_total > 11      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 33
1 2 3 4 range
A 2.3
(-2.8)
A 		20.1
(0.4)
A 		1.2
(-3)
A 		34.1
(3)
A 		33
B 17.1
(0.7)
A 		4.5
(-3)
A 		9.8
(-1.4)
A 		25
(3)
A 		21
C 4.3
(-2.7)
A 		2.8
(-3)
A 		2.8
(-3)
A 		32.7
(3)
A 		30
avr 7.9
(-2.2) 		9.1
(-2) 		4.6
(-3) 		30.6
(3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 22.3
(2.9)
A 		2.6
(-3)
A 		14.7
(0.6)
A 		22.8
(3)
A 		6.3
(-1.9)
A 		22.4
(2.9)
A 		20
B 3.6
(-2.2)
A 		19
(3)
A 		2.9
(-2.4)
A 		2.9
(-2.4)
A 		2.7
(-2.5)
A 		1.2
(-3)
A 		18
C 6.3
(-0.2)
A 		3.7
(-1.6)
A 		1.6
(-2.7)
A 		5.1
(-0.8)
A 		12.1
(3)
A 		1.1
(-3)
A 		11
avr 10.7
(3) 		8.4
(-0.2) 		6.4
(-3) 		10.3
(2.4) 		7
(-2.2) 		8.2
(-0.5) 		4
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5
1 2 3 4 range
A 0.8
(-2.4)
A 		4.4
(2.2)
A 		0.3
(-3)
A 		5
(3)
A 		5
B 5.5
(3)
A 		1.1
(-3)
A 		2.3
(-1.4)
A 		5.3
(2.7)
A 		4
C 1.4
(-2.2)
A 		0.8
(-3)
A 		1
(-2.7)
A 		5.5
(3)
A 		5
avr 2.6
(-1) 		2.1
(-1.7) 		1.2
(-3) 		5.3
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6
1 2 3 4 5 6 range
A 4.1
(0.9)
A 		0.9
(-3)
A 		3.2
(-0.2)
A 		5.8
(3)
A 		1.9
(-1.8)
A 		5.5
(2.6)
A 		5
B 1.2
(-2.3)
A 		6.3
(3)
A 		0.7
(-2.8)
A 		0.8
(-2.7)
A 		1.1
(-2.4)
A 		0.5
(-3)
A 		6
C 2.3
(0.3)
A 		1.1
(-1.8)
A 		0.4
(-3)
A 		1
(-2)
A 		3.9
(3)
A 		0.4
(-3)
A 		4
avr 2.5
(1.7) 		2.8
(3) 		1.4
(-3) 		2.5
(1.7) 		2.3
(0.9) 		2.1
(0) 		1
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180034_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8188
Sequence Type Exemplar sequence
Representative Public ID T20H9.1 NCBI
Target Description T20H9.1 /REP_DB=WormBase Gene ID /WP=CE13856 /TR=O44892 /GB=AAB95050.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065122(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065122 NCBI Caenorhabditis elegans T20H9.1 (T20H9.1) mRNA, complete cds. 11/11 None
SNAP00000029855 ENSEMBL cdna:SNAP chromosome:CEL140:III:2249960:2251436:-1 11/11 None
GENEFINDER00000029863 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2249960:2251436:-1 11/11 None
T20H9.1 ENSEMBL cdna:known chromosome:CEL140:III:2249960:2251692:-1 gene:T20H9.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2249959-2251692(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T20H9.1
Entrez Gene 188664 Entrez gene
SwissProt O44892 EMBL-EBI
WormBase CE13856 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB95050 Hypothetical protein T20H9.1 [Caenorhabditis elegans] ref|NP_497523.1| T20H9.1 [Caenorhabditis elegans] 1.0E-176
blast AAB95050 Hypothetical protein T20H9.1 [Caenorhabditis elegans] ref|NP_497523.1| T20H9.1 [Caenorhabditis elegans] 1.0E-160
blast AAY55894 Hypothetical protein Y54F10BM.15 [Caenorhabditis elegans] ref|NP_001022910.1| Y54F10BM.15 [Caenorhabditis elegans] 9.0E-54
blast AAY55894 Hypothetical protein Y54F10BM.15 [Caenorhabditis elegans] ref|NP_001022910.1| Y54F10BM.15 [Caenorhabditis elegans] 2.0E-43
blast CAB03494 Hypothetical protein ZC47.6 [Caenorhabditis elegans] ref|NP_497363.1| ZC47.6 [Caenorhabditis elegans] 2.0E-43
Protein Domains
Database ID Description E-Value
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 1.8E-46
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 1.8E-46
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 1.4E-10
Sequence
Target Sequence 
>C. ELEGANS:180034_AT
ttattgaccaagctgcctgggctactcaatttatagacggcgccttgaaaggaaatgcat
gtgtgtttgtcgagaagctggaacttcacaagctcttgtttagtgagatccattcaatct
taccatattttgatgcacaatcactaagaaaagttcaattgtgggaaactgatgtatggg
gccaagacccgaacgcagaggagaatttcgaacggattacacagatggaacagtggcaaa
atgtaaaaacacttggtttccaaggcaccgcgtttaagttttcaattgagactctttttc
attttgaaagcctttctgttgaatttgattttttcacagaggaaaatgcaatcaaaatta
gagatgatctcatgaaaagagatacatttcgaagatgcgaggtctggtttaattctagtt
ggttctcaacggcacaaacatttgatccggacggatatcatggccatgaatatgacatag
attattcaaatgatgatggcgacgacttttcaattcgctgtaaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTATTGACCAAGCTGCCTGGGCTAC 354 671 356 Antisense
GGGCTACTCAATTTATAGACGGCGC 318 493 374 Antisense
GAAGCTGGAACTTCACAAGCTCTTG 573 343 429 Antisense
GTGAGATCCATTCAATCTTACCATA 110 483 458 Antisense
TGTATGGGGCCAAGACCCGAACGCA 565 557 528 Antisense
ACACTTGGTTTCCAAGGCACCGCGT 112 111 604 Antisense
GGCACCGCGTTTAAGTTTTCAATTG 248 535 619 Antisense
GCGAGGTCTGGTTTAATTCTAGTTG 432 285 752 Antisense
GTTGGTTCTCAACGGCACAAACATT 412 435 773 Antisense
GATCCGGACGGATATCATGGCCATG 273 415 799 Antisense
GACGACTTTTCAATTCGCTGTAAAC 225 379 856 Antisense
Sequence Source Affymetrix Proprietary Database