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[180028_at]

Description F22E5.9 /REP_DB=WormBase Gene ID /WP=CE09561 /TR=O16705 /GB=AAB66173.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 79      6grp_high1_10_10 > 96      4grp_highest_8_8 > 14      4grp_high_8_8 > 22      6grp_highest_nonorm > 92      6grp_total > 97      4grp_total > 17      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 324
1 2 3 4 range
A 114.1
(-3)
P 		231.2
(-0.8)
P 		186.4
(-1.7)
P 		438.1
(3)
P 		324
B 128.6
(-3)
P 		171.6
(-2.1)
P 		214.7
(-1.2)
P 		410
(3)
P 		281
C 169.9
(-3)
P 		197.9
(-2.3)
P 		203
(-2.1)
P 		394.1
(3)
P 		224
avr 137.5
(-3) 		200.2
(-1.6) 		201.4
(-1.6) 		414.1
(3) 		277

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 648
1 2 3 4 5 6 range
A 128.8
(-3)
P 		169.7
(-2.2)
P 		208.7
(-1.4)
P 		234.2
(-0.9)
P 		414.3
(2.6)
P 		432.5
(3)
P 		304
B 171.8
(-3)
P 		197.6
(-2.7)
P 		313.1
(-1.1)
P 		259.5
(-1.8)
P 		487.7
(1.3)
P 		614.4
(3)
P 		443
C 117.8
(-3)
P 		226.1
(-2)
P 		245.3
(-1.8)
P 		280.4
(-1.5)
P 		389.1
(-0.5)
P 		765.9
(3)
P 		648
avr 139.5
(-3) 		197.8
(-2.2) 		255.7
(-1.5) 		258
(-1.5) 		430.4
(0.8) 		604.3
(3) 		465
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 46
1 2 3 4 range
A 41.5
(-3)
P 		50.2
(-0.7)
P 		48.7
(-1.1)
P 		64.1
(3)
P 		23
B 41
(-3)
P 		42.4
(-2.8)
P 		49.7
(-1.9)
P 		87.4
(3)
P 		46
C 56.2
(-3)
P 		56.9
(-2.7)
P 		70.8
(3)
P 		66.5
(1.2)
P 		15
avr 46.2
(-3) 		49.8
(-2.2) 		56.4
(-0.7) 		72.7
(3) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 233
1 2 3 4 5 6 range
A 23.7
(-3)
P 		60.4
(-0.8)
P 		45.5
(-1.7)
P 		59.4
(-0.9)
P 		125
(3)
P 		105.3
(1.8)
P 		101
B 59.8
(-3)
P 		65.8
(-2.8)
P 		76
(-2.4)
P 		68.7
(-2.7)
P 		198.2
(1.9)
P 		229.1
(3)
P 		169
C 43.6
(-3)
P 		69
(-2.3)
P 		62.4
(-2.5)
P 		56.4
(-2.6)
P 		125.9
(-0.7)
P 		256.4
(3)
P 		213
avr 42.4
(-3) 		65.1
(-2.1) 		61.3
(-2.3) 		61.5
(-2.3) 		149.7
(1.2) 		196.9
(3) 		155
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180028_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5688
Sequence Type Exemplar sequence
Representative Public ID F22E5.9 NCBI
Target Description F22E5.9 /REP_DB=WormBase Gene ID /WP=CE09561 /TR=O16705 /GB=AAB66173.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061913(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061913 NCBI Caenorhabditis elegans F22E5.9 (F22E5.9) mRNA, complete cds. 11/11 None
GENEFINDER00000023633 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2638403:2638993:1 11/11 None
F22E5.9 ENSEMBL cdna:known chromosome:CEL140:II:2638403:2638993:1 gene:F22E5.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2638401-2638992(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F22E5.9
Entrez Gene 184837 Entrez gene
SwissProt O16705 EMBL-EBI
WormBase CE09561 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_494314 F22E5.9 [Caenorhabditis elegans] gb|AAB66173.1| Hypothetical protein F22E5.9 [Caenorhabditis elegans] 1.0E-72
blast NP_494466 Y27F2A.6 [Caenorhabditis elegans] gb|AAF60473.1| Hypothetical protein Y27F2A.6 [Caenorhabditis elegans] 2.0E-26
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494314.1 1 136-153
F22E5.9 1 136-153
GENEFINDER00000023633 1 136-153
Sequence
Target Sequence 
>C. ELEGANS:180028_AT
gtggcaaaaacagttccgagcgcagaagattcgatgaaaggcgtgaaaaatgcggcttcc
aaaatgcgcgccggtgtcgatgaaattgtggaaaaacgaaaattatacgcggaatctgag
actgcaaaactcaaaaaatcactgaaagatctatcgaaggaaaattccgatttaaaaatg
caaaacctgaatctttccgccgaaaattcagatttaaaatccagaggatggctgagcata
tttaaatcgccaaaaaccgagccagaatcccgaaaatcgtcgaaaaaatgggctctggcg
aagaagatgccacaaattttcacaatttttgcaaccgccacctaccttgccaaaaccgtc
agaaaagtcgtcttcatcactcct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGGCAAAAACAGTTCCGAGCGCAG 645 491 61 Antisense
AAATGCGGCTTCCAAAATGCGCGCC 314 119 108 Antisense
ATGCGCGCCGGTGTCGATGAAATTG 124 41 124 Antisense
ATTATACGCGGAATCTGAGACTGCA 89 13 162 Antisense
TGCAAAACCTGAATCTTTCCGCCGA 399 575 239 Antisense
GAGCCAGAATCCCGAAAATCGTCGA 264 383 319 Antisense
ATCGTCGAAAAAATGGGCTCTGGCG 156 35 336 Antisense
GAAGATGCCACAAATTTTCACAATT 338 337 363 Antisense
TTTCACAATTTTTGCAACCGCCACC 416 673 378 Antisense
TACCTTGCCAAAACCGTCAGAAAAG 113 637 403 Antisense
CAGAAAAGTCGTCTTCATCACTCCT 17 205 420 Antisense
Sequence Source Affymetrix Proprietary Database